Genetic diversity of bitter gourd (Momordica charantia L.) genotypes revealed by RAPD markers and agronomic traits

Dey, S. S.; Singh, Ashok Kumar; Chandel, D.; Behera, Tusar Kanti

doi:10.1016/j.scienta.2006.03.006

Cited by 86 publications

(74 citation statements)

References 16 publications

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“…The fruits are eaten while still green and unripe. They have been used for generations by indigenous populations in Africa, India, and Latin America for food and folk medicine (Khan and Anderson, 2003;Dey et al, 2006;Lako et al, 2007;Abo et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Antioxidative properties of bitter gourd (Momordica charantia) and zucchini<br>(Cucurbita pepo)

Hamissou

Smith

Carter

et al. 2013

Emir. J. Food Agric

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The enzymatic and non-enzymatic antioxidative capacities of bitter gourd (Momordica charantia) and zucchini (Cucurbita pepo) were investigated in water extracts and chemical buffer extracts. Bitter gourd and zucchini fruits were purchased from a farmer's market and homogenized separately in water and in a native enzyme extraction buffer. Total phenolic compounds, free radical DPPH scavenging activity, SOD activity and β-glucosidase activity were assayed in the extracts. The average total phenolic compounds recorded in bitter gourd were 13.28 GAE/g fresh weights while in zucchini, the average was 8.67GAE/g fresh weight. This study also found that bitter gourd was 82.05% as effective as ascorbic acid in inhibiting the free radical DPPH while zucchini was 12.19% as effective. The results indicated that bitter gourd was significantly higher in antioxidant content and in β-glucosidase activities than zucchini (P<0.05). On the other hand, significantly higher SOD activities were recorded in zucchini than in bitter gourd extracts.

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Section: Introductionmentioning

confidence: 99%

Antioxidative properties of bitter gourd (Momordica charantia) and zucchini<br>(Cucurbita pepo)

Hamissou

Smith

Carter

et al. 2013

Emir. J. Food Agric

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“…Depending on the strategy adopted, all these can in turn make RAPD a useful technique in plant cultivar and variety identification to varying degrees as shown by recent studies using the manual cultivar identification diagram (MCID) strategy in different crops Zhao et al, 2011;Wang et al, 2011;Li et al, 2011). RAPD markers have also been successfully applied in determining genetic relationships in vegetables such as Capsicum (Bhadragoudar and Patil, 2011), broccoli (Lu et al, 2009), radish (Liu et al, 2008), and curcurbits (Dey et al, 2006;Sureja et al, 2006;Verma et al, 2007), among other crops.…”

Section: Introductionmentioning

confidence: 99%

A novel and efficient strategy for practical identification of tomato (Solanum lycopersicon) varieties using modified RAPD fingerprints

Korir

Li²,

Leng³

et al. 2013

Genet. Mol. Res.

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ABSTRACT.Tomato breeding and variety development have led to the generation of a large number of varieties in many countries worldwide. This has created a growing and urgent need for an improved strategy for genotyping and identification since the traditional methods based on phenotype are growing unreliable. DNA markers could provide distinct benefits in tomato variety identification; however, DNA fingerprint analyses have not made DNA marker data readily usable for identification of varieties in tomato and other crops. A manual cultivar and/or variety identification diagram (MCID) strategy has been developed and has been found to make DNA markers more usable for the identification of genotyped plant individuals. We adopted this strategy, using modified RAPD markers to identify 42 tomato varieties from different geographical origins and seed merchants. All of the varieties were clearly separated and individually identified by reproducible fingerprints of only 6 RAPD primers. The tomato MCID that is generated is usable for the identification of any two or more tomato varieties. In addition, fewer primers can be used to make a distinction between varieties using this approach, since the selected fingerprints from each primer are used after they have been generated. The information in this 1817 ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 12 (2): 1816-1828 (2013 An efficient strategy for identification of tomato varieties first version of the tomato MCID can be enriched through identification and incorporation of more varieties and adaptation to other molecular markers in order to provide a more comprehensive tomato variety identification service for the horticultural industry.

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“…Bitter melon (Memoridica charantia L.) is also known as bitter gourd and is grown in tropical and subtropical climates of India, Pakistan, China and other South Asian countries as vegetable for food and medicinal purposes [1][2][3]. It has a bitter taste but is still considered a culinary delicacy in Indian-subcontinent for its special taste and high nutritive value, and therefore, it is generally sold as a premium priced vegetable [1][2][3][4].…”

Section: Introductionmentioning

confidence: 99%

“…It has a bitter taste but is still considered a culinary delicacy in Indian-subcontinent for its special taste and high nutritive value, and therefore, it is generally sold as a premium priced vegetable [1][2][3][4].…”

Section: Introductionmentioning

confidence: 99%

“…For example, molecular techniques have been developed to identify different cultivars and then in vitro cultivation techniques were developed for mass propagation of selected cultivars and to use these methods for genetic transformation [1,17,18]. Grafting techniques have been developed to produce cultivars tolerant to flooding, and hormonal roles have been tested for manipulating budding and flowering in bitter melon [19][20][21].…”

Section: Introductionmentioning

confidence: 99%

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The effect of leaf presence on the rooting of stem cutting of bitter melon and on changes in polyamine levels

Malik¹,

Pérez²,

Kunta³

2012

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The study was conducted to investigate the optimal hormone treatment for rooting in bitter melon and the effect of defoliation on rooting and polyamine levels. Commercial preparation (diluted 1:10 and 1:20) gave extensive rooting within five days after treatment. The presence of leaf with the stem cutting was necessary for optimal rooting as defoliation drastically reduced rooting even when the rooting time was extended from 5 to 10 days. The presence of leaf also promoted the levels of putrescine three days after the hormone treatment just at the time of root emergence. Cuttings that did not have leaf did not show an increase in putrescine levels and did not produce roots. Spermidine levels also increased in the cuttings that rooted but there was no change in the levels of spermine in the cuttings that rooted. It appears that putrescine and spermidine play a role in regulating rooting in bitter melon cuttings. The vegetative propagation conditions described show that it is a practically viable technique for small growers to produce large number of plantlets at the farm for selection and cultivation.

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Genetic diversity of bitter gourd (Momordica charantia L.) genotypes revealed by RAPD markers and agronomic traits

Cited by 86 publications

References 16 publications

Antioxidative properties of bitter gourd (Momordica charantia) and zucchini<br>(Cucurbita pepo)

Antioxidative properties of bitter gourd (Momordica charantia) and zucchini<br>(Cucurbita pepo)

A novel and efficient strategy for practical identification of tomato (Solanum lycopersicon) varieties using modified RAPD fingerprints

The effect of leaf presence on the rooting of stem cutting of bitter melon and on changes in polyamine levels

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