Genetic diversity of bats coronaviruses in the Atlantic Forest hotspot biome, Brazil

Góes, Luiz Gustavo Bentim; Campos, Angélica Cristine de Almeida; Carvalho, Catherine Teixeira de; Ambar, Guilherme; Queiroz, Luzia Helena; Cruz‐Neto, Ariovaldo P.; Munir, Muhammad; Durigon, Edison Luiz

doi:10.1016/j.meegid.2016.07.034

Cited by 33 publications

(35 citation statements)

References 19 publications

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“…The nested PCR assay with degenerate consensus primers was used to many studies. This assay has been used for the identification and differentiation of CoV in the four genera d e s c r i b e d t o d a t e ( a l p h a -, b e t a -, g a m m a -, a n d deltacoronavirus) [17,19,20]. Similarly to our report, most of avian CoV diversity studies detected the same coronavirus genera [2,17,21,22], with the exception of a report of betacoronavirus detection in distinct birds species from a rehabilitation center in Brazil [8].…”

Section: Discussionmentioning

confidence: 99%

Divergent coronaviruses detected in wild birds in Brazil, including a central park in São Paulo

et al. 2019

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Coronaviruses are single-stranded positive-sense RNA viruses associated with important avian diseases. Their relatively high rates of mutation and recombination frequencies allow them to adapt to new hosts and ecological niches. Although Brazil has 18% of global avian species diversity, studies regarding the presence of avian viral diseases in wild birds in South America are scarce. In this study, we performed a retrospective analysis of the presence of CoVs in 746 wild birds. Oropharyngeal and cloacal swabs were obtained and placed together in vials containing VTM transport medium collected in different regions of Brazil between 2006 and 2013. Screening for viral nucleic acid was performed using conventional RT-PCR and pancoronavirus nested PCR. Positive samples were characterized by partial sequencing of the RNA-dependent RNA polymerase (RdRp) gene, and ensuing phylogenetic analysis was performed to investigate the association between virus epidemiology and bird migration routes. Coronavirus RNA were detected and sequenced from six samples, in which three were related to gammacoronaviruses group and the other three to deltacoronavirus group. Our study documents the presence of CoVs related to avian gamma-and deltacoronaviruses circulating in both urban-and poultry-farm regions of Brazil, implicating wild birds as potential carriers of CoVs which may represent a risk to poultry farms and public health in Brazil.

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Section: Discussionmentioning

confidence: 99%

Divergent coronaviruses detected in wild birds in Brazil, including a central park in São Paulo

et al. 2019

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“…Numerous viruses have been reported to infect bats, and these animals are being studied as potential reservoir for several zoonotic diseases, including Coronaviruses [3,9,10]. The majority of Bat Coronavirus (BtCoV) have been identified in bats from Asia, Africa, and Europe [11][12][13][14][15][16][17][18][19]; however, CoV has also been reported in bats from South American countries including Brazil, Trinidad, Costa Rica, Ecuador, Panama, and Mexico [20][21][22][23][24][25].…”

Section: Introductionmentioning

confidence: 99%

Alphacoronavirus Detection in Lungs, Liver, and Intestines of Bats from Brazil

et al. 2019

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Bats are flying mammals distributed worldwide known to host several types of Coronavirus (CoV). Since they were reported as the probable source of spillover of highly pathogenic CoV into the human population, investigating the circulation of this virus in bats around the world became of great importance. We analyzed samples from 103 bats from two distinct regions in Brazil. Coronavirus from the Alphacoronavirus genus was detected in 12 animals, 11 from São José do Rio Preto-SP region and 1 from Barreiras-BA region, resulting in a prevalence of 17.18% and 2.56% respectively. The virus was detected not only in intestines but also in lungs and liver. Phylogenetic analysis based on nsP12 genomic region suggests that the sequences group according to host family and sampling location. Studies on the circulation of these viruses in bats remain important to understand the ecology and evolutionary relationship of these pathogens.

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“…In Brazil, adenoviruses, anelloviruses, circoviruses, coronaviruses, polyomaviruses, parvoviruses and rhabdoviruses have already been detected in different bat species [16,20,[27][28][29][30]. However, previous studies were performed using traditional genome amplification methods.…”

Section: Introductionmentioning

confidence: 99%

Detection of adenovirus, papillomavirus and parvovirus in Brazilian bats of the species Artibeus lituratus and Sturnira lilium

et al. 2019

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Bats play a significant role in maintaining their ecosystems through pollination, dispersal of seeds, and control of insect populations, but they are also known to host many microorganisms and have been described as natural reservoirs for viruses with zoonotic potential. The diversity of viruses in these animals remains largely unknown, however, because studies are limited by species, location, virus target, or sample type. Therefore, the aim of this study was to detect fragments of viral genomes in bat samples. We performed high-throughput sequencing analysis and specific PCR and RT-PCR on pools of anal and oropharyngeal swabs from Artibeus lituratus and Sturnira lilium collected in southern Brazil. As a result, a member of the family Adenoviridae related to human adenovirus C was detected in anal swabs from S. lilium. In addition, we detected a papillomavirus in an anal swab from A. lituratus. Our analyses also allowed the detection of adenoviruses and parvoviruses in oropharyngeal swabs collected from A. lituratus. These results increase our knowledge about viral diversity and illustrate the importance of conducting virus surveillance in bats.

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Genetic diversity of bats coronaviruses in the Atlantic Forest hotspot biome, Brazil

Cited by 33 publications

References 19 publications

Divergent coronaviruses detected in wild birds in Brazil, including a central park in São Paulo

Divergent coronaviruses detected in wild birds in Brazil, including a central park in São Paulo

Alphacoronavirus Detection in Lungs, Liver, and Intestines of Bats from Brazil

Detection of adenovirus, papillomavirus and parvovirus in Brazilian bats of the species Artibeus lituratus and Sturnira lilium

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