Genetic diversity of Bradyrhizobium japonicum field population revealed by RAPD fingerprinting

Abstract

S. SIKORA, S. REDŽ EPOVIĆ , I. PEJIĆ A N D V . K O ZU MP L IK . 1997. RAPD fingerprinting was used for strain identification and the assessment of genetic diversity within a field population of Bradyrhizobium japonicum. Total genomic DNAs from 13 field isolates and two inoculant strains were amplified using six different 10-mer primers. Different and informative band patterns were obtained for all strains analysed. Cluster analysis unexpectedly revealed that none of the field isolates was identical to inoculant strains which were regularly used for soybean inoculation. Among field isolates two highly divergent groups were determined. The results indicate that RAPD is a very discriminative and efficient method for differentiating and studying genetic diversity of B. japonicum strains.

Characterization of native Bacillus thuringiensis strains by PCR-RAPD based fingerprinting

“…Seventy B. thuringiensis strains subjected to genetic diversity determination using RAPD as a marker. Of these 10 random decamer primers were utilized and were further used to detect the genetic diversity and differentiation of these strains according to the source of origin (Tables 1 and 2) [7,19]. Our results clearly differentiated B. thuringiensis isolates based on their location of origin.…”

“…The RAPD analysis is considered to be a fast and simple method. Once the primers revealing the polymorphism were identifi ed and PCR conditions optimized, slight differences in primer sequences caused signifi cantly different RAPD patterns and enabled the easy discrimination among the strains [7]. In comparison to other molecular typing methods, RAPD is faster, reproducible and less labor-intensive, and eliminates the need for pure DNA.…”

“…RAPD analysis is considered an important molecular biology technique, which is used for the identification of indigenous B. thuringiensis isolates. In comparison to other molecular typing methods, RAPD is faster, less labor-intensive and eliminates the need for pure DNA; only a small amount of template DNA is required for amplifi cation reaction [7,19]. Seventy B. thuringiensis strains subjected to genetic diversity determination using RAPD as a marker.…”

“…Native B. thuringiensis isolates, retrieved from different locations (Table 1), were subjected to randomly amplifi ed polymorphic DNA (RAPD) marker-based analysis for characterization of their genetic diversity. Recently, various techniques that rely on different nucleic acid pattern and discriminate at genetic level have been developed to gain information about the genetic diversity and genetic relationship between different organisms [5][6][7]. The RAPD markerbased analysis was found to be an easy, quick and reliable technique to assess the diversity of different types of organisms [8,9] and this technology was successfully applied to characterize the genetic diversity in various B. thuringiensis isolates [10].…”

Molecular characterization of diazotrophic bacteria isolated from rhizosphere of wheat cropping system from central plain region of Punjab

“…Now-a-days a variety of molecular based methods are used for characterization and identification of bacteria. These includes amplified ribosomal DNA restriction analysis (ARDRA) , random amplified polymorphic DNA (RAPD) (Sikora et al, 1997), repetitive extragenic palindromic elements (REP) (Naik et al, 2008) and 16-23S intergenic spacer . Keeping all these points in view, the objective of the present study was to study the diazotrophic diversity in rhizospheric soil of wheat-based cropping systems of Punjab to ascertain soil health and fertility.…”