Genetic diversity of Austrian pine (Pinus nigra Arnold) populations in Serbia revealed by RAPD

Abstract

The ability of random amplified polymorphic DNA (RAPD) to distinguish among populations of Austrian pine from Serbia was evaluated. Ten arbitrary 10mer primers employed in the analysis produced 113 fragments of which 100 were polymorphic (88.5%). All populations had specific RAPD phenotypes. Certain rare and genotype specific bands were identified which could be effectively used to distinguish the populations. Polymorphism in RAPD markers among P. nigra population was high and sufficient to distinguish each of the populations. The results obtained suggest that RAPD markers are valuable for the genetic diversity estimation in Pinus nigra and for the study of the divergence among populations

Uniform drought and warming responses in Pinus nigra provenances despite specific overall performances

“…P. nigra is known to exhibit a relatively high genetic diversity (Jagielska et al, 2007;Soto et al, 2010), with highest intra-provenance diversity in Serbian provenances (ssp. nigra ARNOLD) (Lucic et al, 2010) and lowest in Corsican provenances (ssp. laricio MAIRE), most probably due to pleistocene geographic isolation (Aguinagalde et al, 1997).…”

Genetic patterns in <i>Pinus nigra</i> from the central Balkans inferred from plastid and mitochondrial data

“…However, those from the Balkans and Turkey, which are larger and more abundant, have occasionally been studied from various aspects (e.g. Liber et al 2003;Lučić et al 2010;Gülsoy et al 2014) but rarely from a population genetics aspect (e.g. Naydenov et al 2006Naydenov et al , 2015Šarac et al 2015).…”

Portuguese Pinus nigra J.F. Arnold populations: genetic diversity, structure and relationships inferred by SSR markers

“…The hypothetic infraspecific taxonomic classification that we propose for the Portuguese planted P. nigra populations is partially supported by a phenotypic characterisation realised in the past century, by Louro (1982). This author considered that the Portuguese P. nigra populations existent at that time belong to the subspecies laricio, salzmanni and nigra.…”

“…Knowledge of genetic diversity can give insights about the adaptive potential and/or the evolutionary history of a species, being particularly crucial in trees with a long life cycle (Hamrick 2004;Giovannelli et al 2017). The gathering of genetic diversity and structure information of forestry populations is helpful for the development of suited and effective strategies of conservation, management, germplasm use and genetic breeding (Frankham et al 2002;Hamrick 2004;Lučić et al 2010). Different molecular marker systems have been performed in various pine species, including P. nigra, to assess the genetic diversity, provenances, genetic structure and relationships, and/or to discriminate closely related taxa (Vendramin et al 1996;Liber et al 2003;Jiménez et al 2005;Naydenov et al 2006;Afzal-Rafii and Dodd 2007;Soto et al 2010;Rubio-Moraga et al 2012;Liu et al 2012;Šarac et al 2015;Bonavita et al 2016;Cipriano et al 2016;Giovannelli et al 2017;Dias et al 2019).…”