Genetic diversity of Acinetobacter spp. adapted to heavy metal polluted environments

Šipošová, Nikola Š; Liptáková, Veronika; Kvasnová, Simona; Kosorínová, Petra; Pristaš, Peter

doi:10.1515/nbec-2017-0006

Cited by 13 publications

(13 citation statements)

References 8 publications

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“…The genomic DNAs of the P. aeruginosa clinical isolates were extracted by alkaline lysis method using Sodium Dodecyl Sulfate (SDS) and NaOH. 12 First, 3-5 colonies of the overnight grown bacteria were suspended in 60 μL of an alkaline solvent (0.5 gr SDS and 0.4 gr NaOH dissolved in 200 μL distilled water), then warmed at 95°C for 10 minutes. Next, centrifuged at 13000 rpm for 5 min, and 180 μL distilled water added to the mixture.…”

Section: Genomic Dna Extractionmentioning

confidence: 99%

Distribution and Molecular Characterization of Resistance Gene Cassettes Containing Class 1 Integrons in Multi-Drug Resistant (MDR) Clinical Isolates of Pseudomonas aeruginosa

Ahmadian¹,

Haghshenas²,

Mirzaei³

et al. 2020

IDR

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The integrons, as the mobile exogenous elements, play a prominent role in the spreading of antimicrobial resistance genes from Pseudomonas aeruginosa clinical isolates to other bacteria. This study aimed to investigate the frequency of class 1 integrons andresistance gene cassettes carrying by them in clinical isolates as well as multidrug resistant P. aeruginosa. Materials and Methods: A total of 100 clinical isolates of P. aeruginosa were collected from 5 hospitals in Mazandaran province, north Iran. The antibiotic susceptibility pattern of the isolates was evaluated using the disk agar diffusion method. Genomic DNAs were extracted and then the presence of class 1 integrons was detected by the PCR test. All PCR products of the positive isolates were sequenced for the detection of resistance gene cassettes by the Sanger method. Results: Forty-one percent of the clinical isolates were multi-drug resistant. Also, 42% of the isolates were contained class 1 integron, and 61.9% of the integron positive isolates were detected as MDR. We detected 10 different gene cassettes sizing from 0.6 to 3.5 kb in the present study. The sequencing analysis of the internal variable regions of the class 1 integrons showed that the 0.75 kb gene cassette (aadB) was the most frequent resistance gene (54.76%) among all clinical isolates, as well as the MDR isolates. Other resistance genes detected in this study were included: aadA6-orfD (35.71%), aacA4-bla OXA-10 (21.42%), aadB-aacA4-bla OXA-10 (19.04%), bla OXA-10-aacA4-VIM1 (11.9%), aacA4-cat B10 (7.14%), aacA5-aadA1-cmlA5 (7.14%), bla OXA31-aadA2 (4.76%), and aac(3)-Ic-aac A5-cmlA5 (4.76%). To the best of our knowledge, bla OXA-10-aacA4-VIM1 cassette array is detected for the first time in this study. Conclusion: The treatment of infections caused by P. aeruginosa in this region of Iran is a major problem due to the high prevalence of class 1 integrons. It seems that the high prescription of beta-lactams and aminoglycosides for the treatment of these infections may be replaced by other combination therapy stewardships.

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Section: Genomic Dna Extractionmentioning

confidence: 99%

Distribution and Molecular Characterization of Resistance Gene Cassettes Containing Class 1 Integrons in Multi-Drug Resistant (MDR) Clinical Isolates of Pseudomonas aeruginosa

Ahmadian¹,

Haghshenas²,

Mirzaei³

et al. 2020

IDR

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“…(isolated from soil and drainage water samples collected from three areas in Slovakia that had been industrially polluted with heavy metals) is high and what is more in different species various numbers of genetic orthologs could be observed. These authors suggested that the presence of plasmids is one of the sources of genetic diversity and that frequently, heavy metal resistance in acinetobacters is plasmid encoded [57]. Other researchers also showed multiple plasmids (plasmidome) encoding resistance to Hg, Cr, Co/ Zn/Cd, Ni, and As in Acinetobcter strains and suggested that these plasmidomes can provide resistance to heavy metals in both environmental and clinical habitats [58][59][60].…”

Section: Plos Onementioning

confidence: 98%

“…In our studies, bacterial representatives belonging to Acinetobacter genus seemed to be in seventh position according to their abundance (0.07-4.9%). Šipošová et al [57] showed that genetic variability in Acinetobacter sp. (isolated from soil and drainage water samples collected from three areas in Slovakia that had been industrially polluted with heavy metals) is high and what is more in different species various numbers of genetic orthologs could be observed.…”

Section: Plos Onementioning

confidence: 99%

Azolla filiculoides L. as a source of metal-tolerant microorganisms

et al. 2020

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The metal hyperaccumulator Azolla filiculoides is accompanied by a microbiome potentially supporting plant during exposition to heavy metals. We hypothesized that the microbiome exposition to selected heavy metals will reveal metal tolerant strains. We used Next Generation Sequencing technique to identify possible metal tolerant strains isolated from the metaltreated plant (Pb, Cd, Cr(VI), Ni, Au, Ag). The main dominants were Cyanobacteria and Proteobacteria constituting together more than 97% of all reads. Metal treatment led to changes in the composition of the microbiome and showed significantly higher richness in the Pb-, Cd-and Cr-treated plant in comparison with other (95-105 versus 36-44). In these treatments the share of subdominant Actinobacteria (0.4-0.8%), Firmicutes (0.5-0.9%) and Bacteroidetes (0.2-0.9%) were higher than in non-treated plant (respectively: 0.02, 0.2 and 0.001%) and Ni-, Au-and Ag-treatments (respectively: <0.4%, <0.2% and up to 0.2%). The exception was Au-treatment displaying the abundance 1.86% of Bacteroidetes. In addition, possible metal tolerant genera, namely: Acinetobacter,

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“…Genes. The alkaline lysis method based on sodium dodecyl sulfate (SDS) (Sigma) and NaOH (Sigma) was used for DNA extraction [16]. Briefly, we solved 0.5 g SDS and 0.4 g NaOH in 200 ml distilled water and suspended the bacterial colony in 20 μl of this extraction buffer.…”

Section: Dna Extraction and Amplification Of Ame-encodingmentioning

confidence: 99%

Role of Aminoglycoside-Modifying Enzymes (AMEs) in Resistance to Aminoglycosides among Clinical Isolates of Pseudomonas aeruginosa in the North of Iran

Ahmadian

Bazgir

Ahanjan

et al. 2021

BioMed Research International

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In recent years, the prevalence of resistance to aminoglycosides among clinical isolates of Pseudomonas aeruginosa is increasing. The aim of this study was to investigate the role of aminoglycoside-modifying enzymes (AMEs) in resistance to aminoglycosides in clinical isolates of P. aeruginosa. The clinical isolates were collected from different hospitals. Disk agar diffusion test was used to determine the antimicrobial resistance pattern of the clinical isolates, and the minimum inhibitory concentration of aminoglycosides was detected by microbroth dilution method. The PCR was performed for discovery of aminoglycoside-modifying enzyme-encoding genes. Among 100 screened isolates, 43 (43%) isolates were resistant to at least one tested aminoglycosides. However, 13 (13%) isolates were resistant to all tested aminoglycosides and 37 isolates were detected as multidrug resistant (MDR). The resistance rates of P. aeruginosa isolates against tested antibiotics were as follows: ciprofloxacin (41%), piperacillin-tazobactam (12%), cefepime (32%), piperacillin (26%), and imipenem (31%). However, according to the MIC method, 13%, 32%, 33%, and 37% of the isolates were resistant to amikacin, gentamicin, tobramycin, and netilmicin, respectively. The PCR results showed that AAC(6 ′ )-Ib was the most commonly (26/43, 60.4%) identified AME-encoding gene followed by AAC(6 ′ )-IIa (41.86%), APH(3 ′ )-IIb (34.8%), ANT(3 ″ )-Ia (18.6), ANT(2 ″ )-Ia (13.95%), and APH(3 ″ )-Ib (2.32%). However, APH(3 ′ )-Ib was not found in any of the studied isolates. The high prevalence of AME-encoding genes among aminoglycoside-resistant P. aeruginosa isolates in this area indicated the important role of AMEs in resistance to these antibiotics similar to most studies worldwide. Due to the transmission possibility of these genes between the Gram-negative bacteria, we need to control the prescription of aminoglycosides in hospitals.

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Genetic diversity of Acinetobacter spp. adapted to heavy metal polluted environments

Cited by 13 publications

References 8 publications

<p>Distribution and Molecular Characterization of Resistance Gene Cassettes Containing Class 1 Integrons in Multi-Drug Resistant (MDR) Clinical Isolates of <em>Pseudomonas aeruginosa</em></p>

<p>Distribution and Molecular Characterization of Resistance Gene Cassettes Containing Class 1 Integrons in Multi-Drug Resistant (MDR) Clinical Isolates of <em>Pseudomonas aeruginosa</em></p>

Azolla filiculoides L. as a source of metal-tolerant microorganisms

Role of Aminoglycoside-Modifying Enzymes (AMEs) in Resistance to Aminoglycosides among Clinical Isolates of Pseudomonas aeruginosa in the North of Iran

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