Genetic Diversity in the Highbush Blueberry Evaluated with Microsatellite Markers

Boches, Peter; Bassil, Nahla V.; Rowland, Lisa J.

doi:10.21273/jashs.131.5.674

Cited by 49 publications

(32 citation statements)

References 60 publications

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“…Microsatellite markers selected for this study were found to be a reliable technique to differentiate the types, cultivars, clones of Vaccinium spp. (Boches et al, 2006;Cesonien_ e et al, 2013;Gajdo sov a et al, 2006), and used to develop phylogenetic relationships among lowbush blueberry clones collected from different provinces of Canada (Debnath, 2014). In this study, polymorphic banding pattern at nine out of 20 SSR loci detected for 'QB 9C' compared with 'Fundy' confirmed the diversification between the wild clone and named cultivar studied, and confirmed the utility of using EST-SSR markers to control the clonal fidelity of micropropagated blueberry plants.…”

Section: Parameterssupporting

confidence: 71%

Propagation Methods Affect Fruit Morphology and Antioxidant Properties but Maintain Clonal Fidelity in Lowbush Blueberry

Goyali¹,

Igamberdiev²,

Debnath³

2015

horts

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Section: Parameterssupporting

confidence: 71%

Propagation Methods Affect Fruit Morphology and Antioxidant Properties but Maintain Clonal Fidelity in Lowbush Blueberry

Goyali¹,

Igamberdiev²,

Debnath³

2015

horts

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“…5). Boches et al (2006a) found that wild V. corymbosum accessions were genetically more diverse than cultivated NHB as assessed by SSR polymorphism. It is likely that this difference was caused in part by a founding event, i.e., when breeders choose only a few genotypes for all subsequent crop improvement (Hyten et al, 2006).…”

Section: Resultsmentioning

confidence: 98%

Impact of Wide Hybridization on Highbush Blueberry Breeding

Brevis¹,

Bassil²,

Ballington³

et al. 2008

J. Amer. Soc. Hort. Sci.

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The use of interspecific hybridization in blueberry (Vaccinium L. section Cyanococcus Gray) breeding has resulted in the incorporation of novel traits from wild germplasm and the expansion of the geographic limits of highbush blueberry (V. ×corymbosum L.) production. The objectives of this study were: 1) to estimate the impact of wide hybridization on inbreeding, heterozygosity, and coancestry of the cultivated tetraploid highbush blueberry; 2) to establish the usefulness of microsatellite markers in assessing genetic relationships among southern highbush blueberry [SHB (V. ×corymbosum)] cultivars; and 3) to report on the expected genetic contribution of wild Vaccinium clones to SHB cultivars. Pedigrees of 107 highbush blueberry cultivars were used to calculate tetrasomic inbreeding coefficients (F), pedigree-based genetic distances, and expected genetic contributions of wild clones. Genotyping data from 21 single-locus microsatellite markers screened across 68 genotypes were used to calculate heterozygosity and proportion of shared alleles distances (Dsa). The results indicated that the effects of wide hybridization on genetic diversity of cultivated blueberry were lower than previously thought. First, SHB cultivars exhibited similar levels of molecular relatedness as historical northern highbush blueberry [NHB (V. ×corymbosum)] cultivars (median Dsa between pairs of cultivars equals 0.19 in both cultivar groups), despite the broader genetic base and larger pedigree distances in the former cultivar group. Second, levels of heterozygosity in modern NHB cultivars were not statistically different from those of SHB (χ2 = 4.0; df = 3; P > 0.05), despite the considerably higher levels of inbreeding in the former cultivar group (median F equal to 0.0035 and 0.0013, respectively). Furthermore, pedigree-based genetic distances were significantly correlated with Dsa (r = 0.57, P ≤ 0.0001), indicating that microsatellite markers are reliable tools in most cases to assess the genetic relationships among SHB cultivars. Finally, seven Vaccinium species constitute the current genetic base of cultivated SHB. In this article, we report on the expected genetic contributions of V. angustifolium Ait., V. constablaei Gray, V. corymbosum, V. darrowii Camp, V. elliottii Chapman, V. tenellum Ait., and V. virgatum Ait. (syn. V. ashei Reade) clones to 38 SHB cultivars.

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“…This could also be due to the difference in the genetic background of the two berry types. Differences may include varying pectin content, native enzymes, sugar and water content, skin thickness, as well as the effect pectinase enzymes have on the intercellular structures of the berries (Boches et al, 2006;Gerard and Roberts, 2004;Pedrolli et al, 2009;Silva et al, 2005).…”

Section: Resultsmentioning

confidence: 99%

Not-from-concentrate Blueberry Juice Extraction Utilizing Frozen Fruit, Heated Mash, and Enzyme Processes

Stein-Chisholm

Finley

Losso

et al. 2017

hortte

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Juice production is a multibillion dollar industry and an economical way to use fruit past seasonal harvests. To evaluate how production steps influence not-from-concentrate (NFC) blueberry (Vaccinium sp.) juice recovery, bench top and pilot scale experiments were performed. In bench-top, southern highbush (SHB) blueberry (Vaccinium darrowii × Vaccinium corymbosum) and rabbiteye blueberry (RAB) (Vaccinium ashei) were pressed at varying temperatures. Press treatments included ambient temperature, frozen then thawed, and frozen then heated berries. In addition, two commercial pectinase enzymes were evaluated. Three batches were pressed and average juice recovery was calculated. The highest average free juice recovery (68.8% ± 1.1%) was attained by heating frozen berries and treating with enzyme. Comparing berry species pressed, SHB blueberries produced significantly more juice than RABs. There were no significant differences between enzymes used between berry species. Using this preliminary data, the optimum juice recovery method was then transferred to pilot scale processing. RABs were heated and treated with enzyme then pressed. Free juice recovery from the pilot scale was 74.0% ± 1.0%. Total juice recovery was calculated to be 87% ± 0.6%. With this information, further refinement of juice processes could increase juice production output for small-scale producers and expand local outlets for growers to market their crops as well as create new opportunities for growth in the fresh juice market segment.

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Genetic Diversity in the Highbush Blueberry Evaluated with Microsatellite Markers

Cited by 49 publications

References 60 publications

Propagation Methods Affect Fruit Morphology and Antioxidant Properties but Maintain Clonal Fidelity in Lowbush Blueberry

Propagation Methods Affect Fruit Morphology and Antioxidant Properties but Maintain Clonal Fidelity in Lowbush Blueberry

Impact of Wide Hybridization on Highbush Blueberry Breeding

Not-from-concentrate Blueberry Juice Extraction Utilizing Frozen Fruit, Heated Mash, and Enzyme Processes

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