Genetic Diversity in Punica granatum Revealed by Nuclear rRNA, Internal Transcribed Spacer and RAPD Polymorphism

Singh, Sunil Kumar; Meghwal, P.R.; Pathak, Rakesh; Gautam, Ragini; Kumar, Suresh

doi:10.1007/s40009-013-0120-8

Cited by 16 publications

(3 citation statements)

References 35 publications

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“…Moreover, as very little information is available on the genomics of this fruit species, more studies are needed to exploit the great potential of genetic tools. In recent years, an increasing number of works have been focusing on molecular characterization and genetic diversity assessment of Punica granatum by using different classes of markers such as AFLP (Amplified Fragment Polymorphism, Moslemi et al, 2010;Yuan et al, 2007), RFLP (Restriction Fragment Length Polymorphism, Melgarejo et al, 2009), RAPD (Random Amplified Polymorphic DNA, Singh et al, 2013;Sarkosh et al, 2009Sarkosh et al, , 2006 and SSR (Simple Sequence Repeats, Lihua et al, 2013;Parvaresh et al, 2012;Gadaleta et al, 2007;Kazemi Alamuti et al, 2012;Jbir et al, 2012;Hasnaoui et al, 2012Hasnaoui et al, , 2010Pirseyedi et al, 2010;Currò et al, 2010;Ebrahimi et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Characterization of pomegranate (Punica granatum L.) genotypes collected in Puglia region, Southeastern Italy

Ferrara

Giancaspro

Mazzeo

et al. 2014

Scientia Horticulturae

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Section: Introductionmentioning

confidence: 99%

Characterization of pomegranate (Punica granatum L.) genotypes collected in Puglia region, Southeastern Italy

Ferrara

Giancaspro

Mazzeo

et al. 2014

Scientia Horticulturae

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“…The ITS include the ITS1, 5.8S and ITS2 regions of plants. The rapid evolution rate, high variability and bi-parental inheritance make them suitable for genetic diversity analysis at the species levels [13][14][15]. ITS regions have been proven to be particularly effective for the study of interspecific and intraspecific variation in multiple species [14][15][16][17][18][19][20], although ITS sequences have often shown incomplete concerted evolution and this might affect the construction of cluster trees [21,22].…”

Section: Introductionmentioning

confidence: 99%

Potential biological mechanisms underlying the endangered status of Glehnia littoralis revealed by nrDNA ITS and RAPD analyses

Zhang

et al. 2020

Biotechnology & Biotechnological Equipment

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Certain biological mechanisms of Glehnia littoralis, an endangered medicinal plant in China, may be underlying its endangered status and this has attracted extensive attention from researchers. In this study, nuclear ribosomal DNA internal transcribed spacer (nrDNA ITS) sequences and random amplified polymorphic DNA (RAPD) were investigated to determine the genetic diversity of G. littoralis. A total of 291 samples from 11 G. littoralis populations were collected from the east coast of China. The high genetic diversity and large genetic differentiation within populations were reconfirmed by both ITS (Ht ¼ 0.576, Hs ¼ 0.512, G st ¼ 0.1526) and RAPD analyses (Ht ¼ 0.51, Hs ¼ 0.35, G st ¼ 0.31). The phylogenetic trees showed consistent topologies based on ITS and RAPD; all populations were divided into two clades. Mantel tests (ITS: r ¼ 0.3296, p ¼ 0.067; RAPD: r ¼ 0.036, p ¼ 0.418) showed no significant positive correlation between the geographical distance and genetic distance for G. littoralis populations. These results showed that G. littoralis has great evolutionary potential and its status as endangered might be related to special dispersal and germination mechanisms of its seeds, as well as habitat destruction. In conclusion, we suggest that the conservation strategy for G. littoralis should be based on the protection of all the wild populations to the extent possible.

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“…In recent years, different classes of dominant molecular markers such as RAPD (random amplified polymorphic DNA, Sarkosh et al 2009, Singh et al 2013, AFLP (amplified fragment polymorphism, Moslemi et al 2010, Ajal et al 2015, ISSR (inter simple sequence repeats, Narzary et al 2010, Talebi et al 2011, Hasnaoui et al 2012, Noormohammadi et al 2012) and DAMD (directed amplification of minisatellite DNA, Ranade et al 2009) have been widely applied for molecular characterization and genetic diversity assessment of P. granatum in different regions. Among those, ISSR genotyping has been identified as an efficient and reliable tool for investigating DNA polymorphism and assessing the genetic diversity of pomegranates (Narzary et al 2010, Noormohammadi et al 2012, Ajal et al 2014) and, thus, was selected for the current study.…”

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confidence: 99%

Molecular diversity and genetic relationships among Sri Lankan pomegranate Punica granatum landraces assessed with inter simple sequence repeat (ISSR) regions

Attanayake

Kumari²,

Weerakkody

et al. 2017

Nordic Journal of Botany

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Pomegranate Punica granatum was first introduced to Sri Lanka, possibly through ancient trade routes, thousands of years ago. However, there is no information about the diversity of the pomegranate germplasm in the country, which is important both for breeding new varieties and for conservation efforts. We used inter‐simple sequence repeat (ISSR) regions to investigate the genetic diversity and population structure of pomegranate on the island of Sri Lanka. Hundred and twenty accessions representing seven populations from all pomegranate growing regions of the country were analyzed using 20 ISSR primers. A total of 107 loci were amplified with an average polymorphism information content of 0.3. While the average inter‐population genetic distance was 0.141, it was 0.149 between populations, indicating moderate genetic diversity both within and among populations. Analysis of molecular variance and Nei's genetic diversity revealed higher genetic variation within populations than among populations, and low genetic differentiation (GST) in pair‐wise comparison of populations also suggested limited population differentiation. A considerable level of among‐population gene flow (Nm) was indicated, irrespective of geographical structure and distances. The results of cluster analysis was also in agreement with above analysis and suggest human mediated gene flow and migration patterns. Cluster analysis revealed two main population clusters with several sub‐clusters. While these clusters did not show any correlation with geography, all red peeled accessions clustered into a small sub‐cluster. The results indicate that analysis of ISSR variability is sufficiently informative and powerful to assess the genetic diversity of P. granatum landraces in Sri Lanka.

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Genetic Diversity in Punica granatum Revealed by Nuclear rRNA, Internal Transcribed Spacer and RAPD Polymorphism

Cited by 16 publications

References 35 publications

Characterization of pomegranate (Punica granatum L.) genotypes collected in Puglia region, Southeastern Italy

Characterization of pomegranate (Punica granatum L.) genotypes collected in Puglia region, Southeastern Italy

Potential biological mechanisms underlying the endangered status of Glehnia littoralis revealed by nrDNA ITS and RAPD analyses

Molecular diversity and genetic relationships among Sri Lankan pomegranate Punica granatum landraces assessed with inter simple sequence repeat (ISSR) regions

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