Genetic diversity in populations of Uncinula necator: comparison of RFLP- and PCR-based approaches

Stummer, Belinda E.; Zanker, T; Scott, Eileen S.; Whisson, D.L.

doi:10.1017/s0953756299001070

Cited by 33 publications

(35 citation statements)

References 26 publications

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“…Differentiation of genetic markers between subpopulations might be expected to be weak because Santa Cristina and Fornace are separated by only 60 km. Previous studies of E. necator in Europe found that rapid amplification of DNA ends haplotypes were more similar within than between vineyards (7)(8)(9)15), although no such correlations were found in Australia with restriction fragment length polymorphism and ISSR markers (20). In the present study, the overwintering mode is confounded with location, which makes it impossible to determine if genetic differentiation is associated with biological differences in overwintering or with geographic location.…”

Section: Discussioncontrasting

confidence: 66%

“…To address this question, we sampled from subpopulations where we knew the sources of primary inoculum (4) and we sampled these subpopulations at the same time, early in the epidemic. In previous studies (7,9,15,20), isolates recovered from flag shoots (and some leaves [see Miazzi et al {15}]) early in the epidemic were genetically distinct from isolates collected later from foliage and fruit clusters. We did not find that the two subpopulations, which represent distinct overwintering modes and sources of primary inoculum, were genetically distinct.…”

Section: Discussionmentioning

confidence: 91%

“…Uncinula necator), have described two genetically distinct groups, or biotypes, that correspond to the type of primary inoculum or to the time of sampling during an epidemic (7,9,15,20). Group I (sensu Délye) isolates were recovered early in the season from flag shoots, i.e., symptomatic shoots that result from mycelium overwintering in buds that were infected during the previous season (17,18).…”

mentioning

confidence: 99%

“…Thus, the two groups may not correspond strictly to different reproductive and overwintering modes. Although the two groups of E. necator appear to be genetically differentiated, there are reports that these groups are interfertile under laboratory conditions and can produce viable ascospores (5,6,15,20). Mating between groups in the field would eventually homogenize their genetic differences.…”

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confidence: 99%

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Genetic Similarity of Flag Shoot and Ascospore Subpopulations of Erysiphe necator in Italy

Cortesi

Mazzoleni

Pizzatti

et al. 2005

Appl Environ Microbiol

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The overwintering mode of the grape powdery mildew fungus, Erysiphe necator (syn. Uncinula necator), as mycelium in dormant buds (resulting in symptoms known as flag shoots) or as ascospores in cleistothecia, affects the temporal dynamics of epidemics early in the growing season. We tested whether distinct genetic groups (I and III) identified previously in E. necator correlate to overwintering modes in two vineyards in Tuscany, Italy, to determine whether diagnostic genetic markers could be used to predict overwintering. Samples from one vineyard were collected from flag shoots; the other vineyard, 60 km away, had no flag shoots, and mildew colonies were assumed to be derived from ascospores. Genetic markers putatively diagnostic for groups I and III showed that both types were common in the flag shoot subpopulation. Both genetic types were found in the ascospore population, although group III was dominant. We did not find strong genetic differentiation between the two subpopulations based on inter-simple sequence repeat markers. Although there was significant (P < 0.001) genetic differentiation between these subpopulations in 1997 and when 1997 and 1998 subpopulations were pooled ( ‫؍‬ 0.214 and 0.150, respectively), no differentiation was evident between vineyards in 1998 ( ‫؍‬ 0.138, P ‫؍‬ 0.872). Moreover, we did not observe distinct lineages corresponding to overwintering modes, as observed in previous studies. We could not determine if differentiation resulted from biological differences or restricted gene flow between the two vineyards. Our samples were taken from both subpopulations early in the epidemic, while previous studies confounded overwintering mode and sampling time. These results do not support a strong correlation between overwintering and genetic groups, highlighting the need to base population biology studies on sound biological and epidemiological knowledge.

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Section: Discussioncontrasting

confidence: 66%

Section: Discussionmentioning

confidence: 91%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Genetic Similarity of Flag Shoot and Ascospore Subpopulations of Erysiphe necator in Italy

Cortesi

Mazzoleni

Pizzatti

et al. 2005

Appl Environ Microbiol

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“…(Bączkiewicz et al 2008), as well as in studies investigating the population genetics of Uncinula necator (Schwein.) Burrill (Stummer et al 2000), Chamaedaphne calyculata (L.) Moench ). ISSR (Inter Simple Sequence Repeat) markers (Zietkiewicz et al 1994) are the second category of markers applied in this study.…”

Section: Introductionmentioning

confidence: 99%

Molecular studies did not support the distinctiveness of Malva alcea and M. excisa (Malvaceae) in Central and Eastern Europe

et al. 2012

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Relics of Malva alcea are found in Central and Western Europe. A similar taxon, M. excisa, is native to the eastern parts of Europe. According to selected sources, the geographical range boundary of the above taxa intersects Poland. Taxonomic research relying on key morphological features (the depth of corolla petal incisions and the type of hairs covering the stem) did not clearly validate the distinctness of those species. Genetic variation between Malva alcea and M. excisa was analyzed using ISSR and ISJ markers. The performed analysis did not reveal statistically significant differences at the level of genetic diversity between M. alcea and M. excisa populations. The obtained genetic identity values (I = 0.985) do not support the identification of eastern populations as a distinct biological species of M. excisa. The applied DNA markers did not reveal species-specific bands supporting molecular identification of those taxa. The obtained genetic identity values were verified by neighbor-joining grouping which showed that M. alcea and M. excisa did not form corresponding clusters, thus pointing to an absence of significant differences between the analyzed taxa. Differences between the above species were not revealed by an analysis of the sequences of chloroplast regions trnHpsbA and rpoC1, either.

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Assessment of fungicide resistance and pathogen diversity in Erysiphe necator using quantitative real‐time PCR assays

Dufour

Fontaine²,

Montarry

et al. 2010

Pest Management Science

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Genetic diversity in populations of Uncinula necator: comparison of RFLP- and PCR-based approaches

Cited by 33 publications

References 26 publications

Genetic Similarity of Flag Shoot and Ascospore Subpopulations of Erysiphe necator in Italy

Genetic Similarity of Flag Shoot and Ascospore Subpopulations of Erysiphe necator in Italy

Molecular studies did not support the distinctiveness of Malva alcea and M. excisa (Malvaceae) in Central and Eastern Europe

Assessment of fungicide resistance and pathogen diversity in Erysiphe necator using quantitative real‐time PCR assays

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