Genetic diversity in monophasic (1,4,[5],12:i:- and 1,4,[5],12:-:1,2) and in non-motile (1,4,[5],12:-:-) variants of Salmonella enterica S. Typhimurium

Bugarel, Marie; Granier, Sophie A.; Bonin, Emilie; Vignaud, Marie-Léone; Roussel, Sophie; Fach, Patrick; Brisabois, Anne

doi:10.1016/j.foodres.2011.06.057

Cited by 24 publications

(10 citation statements)

References 21 publications

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“…This particular group of strains was also found in one of our previous studies (Bugarel et al. ). Our investigation revealed for the first time the presence of the hin gene in the “inconsistent” S .1,4,[5],12:i:‐ group of strains.…”

Section: Discussionsupporting

confidence: 86%

“…; Bugarel et al. ) as they are characterized by an inconsistent serological detection of the second flagellar phase. These findings could suggest that such strains may have deletions or mutation in the fljB gene out of the primer‐binding site of the fljB marker that modifies protein structure inhibiting serological detection.…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, absence of the phase‐2 protein is not due to the absence of the encoding gene, but should be related to dysfunction in gene expression, in protein translocation, in flagellar filament assembly (Bugarel et al. ).…”

Section: Discussionmentioning

confidence: 99%

“…; Bugarel et al. ). Due to the increasing prevalence of monophasic S .1,4,[5],12:i:‐ isolates, particularly in the food chain, the European Food Safety Authority (EFSA) recently recommended the confirmation of the serological identification of monophasic S .1,4,[5],12:i:‐ strains using a polymerase chain reaction (PCR) protocol based on the detection of the fliA‐B intergenic region and the fljB gene.…”

Section: Introductionmentioning

confidence: 97%

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Molecular identification in monophasic and nonmotile variants of Salmonella enterica serovar Typhimurium

et al. 2012

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Variant strains of Salmonella enterica serovar Typhimurium, lacking one or both flagellar phases have been widely reported. The monophasic S.1,4,[5],12:i:- variant has emerged worldwide in the past few years and has become one of the most frequently encountered in many countries. In contrast, monophasic S.1,4,[5],12:-:1,2 and nonmotile S.1,4,[5],12:-:- strains are rarely described. This study investigated seven molecular markers to identify and delineate monophasic S.1,4,[5],12:i:- (n = 90), S.1,4,[5],12:-:1,2 (n = 25), nonmotile S.1,4,[5],12:-:- (n = 17) strains, and some serovar Typhimurium strains (n = 124) collected through the French Salmonella network between 2001 and 2010. Three markers were commonly detected in serovar Typhimurium and in all variant strains: STM2757, mdh and fliA-B. Monophasic S.1,4,[5],12:i:- were genotypically confirmed by the absence of the fljB, fljA, and hin genes. Nevertheless, 13 (14.5%) of them were positive for these last three genes, revealing monophasic strains named “inconsistent” as previously described. All nonmotile 1,4,[5],12:-:- strains had the fliC, fljA, fljB, and hin genes and the fliC gene was detected in 88% of monophasic S.1,4,[5],12:-:1,2 strains. The combination of the seven markers detection enables to recognize eight different genotypes within the S.1,4,[5],12:i:- collection, among which the Spanish and the U.S. clones previously described could be distinguished and assigned to a genotype. Based on this molecular approach, 71% of the French S.1,4,[5],12:i:- collection belonged to the Spanish clone, whereas only 2% were assigned to the U.S. clone. This study highlights the usefulness of these molecular markers and genotypes for identifying lineages, especially among the epidemiologically important monophasic S.1,4,[5],12:i:- variant.

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Section: Discussionsupporting

confidence: 86%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 97%

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Molecular identification in monophasic and nonmotile variants of Salmonella enterica serovar Typhimurium

et al. 2012

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“…These two consecutive outbreaks appeared in a context of emergence of monophasic variants of Salmonella Typhimurium all over Europe in humans, animals and food products [1,2]. Surveillance data from the French Agency for Food, Environmental and Occupational Health and Safety (Anses), showed that this 4, [5],12:i:variant had been identified in multiple animal and food samples including pork and beef [3]. While this serotype was rarely identified before the mid-1990s, it is now among the most reported Salmonella serotype in the European Union [2,4-7, and personal communication, European Centre for Disease prevention and Control, 17 Jan 2011].…”

Section: Outbreak Descriptionmentioning

confidence: 99%

Nationwide outbreak of Salmonella enterica serotype 4,[5],12:i:- infection associated with consumption of dried pork sausage, France, November to December 2011

et al. 2012

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An outbreak of the monophasic variant of Salmonella enterica serotype 4,[5],12:i:- occurred in November and December 2011 in France. Epidemiological investigation and food investigation with the help of supermarket loyalty cards suggested dried pork sausage from one producer as the most likely source of the outbreak. Despite the absence of positive food samples, control measures including withdrawal and recall were implemented.

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BAC TRAT 2A® biological modulator performed well in the control of Salmonella sp. in production environment and digestive tract of broilers

Kaefer

Santos²,

Tadielo³

et al. 2022

Biological Control

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Genetic diversity in monophasic (1,4,[5],12:i:- and 1,4,[5],12:-:1,2) and in non-motile (1,4,[5],12:-:-) variants of Salmonella enterica S. Typhimurium

Cited by 24 publications

References 21 publications

Molecular identification in monophasic and nonmotile variants of Salmonella enterica serovar Typhimurium

Molecular identification in monophasic and nonmotile variants of Salmonella enterica serovar Typhimurium

Nationwide outbreak of Salmonella enterica serotype 4,[5],12:i:- infection associated with consumption of dried pork sausage, France, November to December 2011

BAC TRAT 2A® biological modulator performed well in the control of Salmonella sp. in production environment and digestive tract of broilers

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