Genetic diversity in<i>Vicia faba</i>L. populations cultivated in Tunisia revealed by simple sequence repeat analysis

Yahia, Yassine; Hannachi, Hédia; Monforte, Antonio J.; Cockram, James; Loumerem, Mohamed; Zarouri, Belkacem; Ferchichi, Ali

doi:10.1017/s1479262114000021

Cited by 8 publications

(7 citation statements)

References 38 publications

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“…In addition, the ICARDA derived lines named with Flip10 and Flip03 prefixes were explicitly separated in this respect. The mean Nei's (1973) gene diversity (0.40) in the faba bean genotypes was similar to that obtained by Yahia et al [30], who reported a mean value of 0.43, using 16 Tunisian faba bean local populations and 16 polymorphic SSR markers. In the present study, mean effective number of alleles (1.67) was lower than that (2.25) obtained by Yahia et al [30] and relatively similar to Wang et al [31], who studied genetic diversity and relatedness of 802 faba bean genotypes using 11 intersimple sequence repeat (ISSR) primers and found a mean value of 1.3888.…”

Section: Resultssupporting

confidence: 85%

Genetic diversity in some faba bean (Vicia faba L.) genotypes assessed by simple sequence repeats

Tufan

Erdoğan

2016

Biotechnology & Biotechnological Equipment

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The success of the breeding programmes depends on the dimension of genetic variability. Simple sequence repeats (SSR) have been extensively used in the studies including genetic characterization of many plant species. The objective of this study was to investigate the genetic variation in 22 faba bean genotypes, 18 of which were originated from International Center for Agricultural Research in the Dry Areas (ICARDA) and 4 of which were cultivated genotypes in Turkey, using SSR markers. Of 41 SSR markers used, 25 produced bands. As a result of SSR amplification, a total of 39 bands, 25 of which were polymorphic and 14 of which were monomorphic, were obtained. The mean gene diversity and polymorphism information content values were 0.27 and 0.24, respectively. The faba bean genotypes cultivated in Turkey had greater genetic diversity than those that originated from ICARDA. The faba bean genotypes FLIP10-and FLIP03-were successfully separated, using the un-weighted pair group method with arithmetic average dendrogram constructed via Jaccard similarity coefficients. These results were further supported by factor analysis substantially. The results indicated that there is sufficient genetic diversity among the tested faba bean genotypes (especially cultivated in Turkey) and could be used in faba bean breeding programmes.

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Section: Resultssupporting

confidence: 85%

Genetic diversity in some faba bean (Vicia faba L.) genotypes assessed by simple sequence repeats

Tufan

Erdoğan

2016

Biotechnology & Biotechnological Equipment

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“…These remarks were also observed for TL6 (local) and TL15 (Romania). This result concurs with those found by Lazrek et al (2009) and Yahia et al (2014), who used molecular markers with plant species (Medicago truncatula and Vicia faba L., respectively) and showed the presence of divergence between genetic clusters and geographical origins for the different cultivars studied. The group C included two introduced accessions (TL12 and TL14).…”

Section: Discussionsupporting

confidence: 92%

Agro-Morphological Variation and Genetic Diversity Assessment of Tunisian Sunflower (Helianthus Annuus L.) Accessions Using Microsatellite Markers

2022

THE JAPS

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Sunflower (Helianthus annuus L.) is considered as one of the most important oilseed crops in the world. In Tunisia, sunflower is cultivated in the Northern region of the country using local populations which are genetically not well studied. This study was conducted to evaluate the genetic variation of 33 accessions (26 local and 7 introduced) using 23 agro-morphological traits and 15 simple sequence repeats (SSR) markers. The variance analysis of the agromorphological traits showed a significant variation among sunflower accessions. The relationship among the sunflower accessions was also performed by using the principal component analysis; the two first axes explained 54.4% of the total variability and showed that the accessions spread into five groups. The groups (G3 and G4) could be used to improve sunflower varieties with high performance especially for the diameter of the head and the weight of 1000 seeds characters. Ten among the 15 SSR primers used revealed clear polymorphic bands and were able to amplify 29 alleles with an average of 2.9 alleles per locus. The percentage of total polymorphism, the polymorphism information content (PIC) and the dissimilarity coefficient values varied from 50 to 100% with an average of 91%, from 0.35 to 0.75 with an average of 0.50, and from 0.00 to 0.81 with an average of 0.41, respectively. Cluster analysis of the SSR markers grouped accessions into 3 distinct groups. A significant correlation was observed among SSR markers and morphological traits. These results found in this study may be helpful for improvement of sunflower breeding programs.

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“…This is in agreement with our SSR results. Also in Tunisia, Yahia, Hannachi [ 53 ] found a correlation between SSR alleles and seed size, fitting the “ major , minor , equina” classification. Likewise, Terzopoulos and Bebeli (8)] could see a separation of minor types from other faba bean types in a PCA plot based on the allele frequency data for four ISSR markers.…”

Section: Resultsmentioning

confidence: 90%

Genetic Diversity and Population Structure in Vicia faba L. Landraces and Wild Related Species Assessed by Nuclear SSRs

et al. 2016

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Faba bean (Vicia faba L.) is a facultative cross-pollinating legume crop with a great importance for food and feed due to its high protein content as well as the important role in soil fertility and nitrogen fixation. In this work we evaluated genetic diversity and population structure of faba bean accessions from the Western Mediterranean basin and wild related species. For that purpose we screened 53 V. faba, 2 V. johannis and 7 V. narbonensis accessions from Portugal, Spain and Morocco with 28 faba bean Single Sequence Repeats (SSR). SSR genotyping showed that the number of alleles detected per locus for the polymorphic markers ranged between 2 and 10, with Polymorphic Information Content (PIC) values between 0.662 and 0.071, and heterozygosity (HO) between 0–0.467. Heterozygosity and inbreeding coefficient levels indicate a higher level of inbreeding in wild related species than in cultivated Vicia. The analysis of molecular variance (AMOVA) showed a superior genetic diversity within accessions than between accessions even from distant regions. These results are in accordance to population structure analysis showing that individuals from the same accession can be genetically more similar to individuals from far away accessions, than from individuals from the same accession. In all three levels of analysis (whole panel of cultivated and wild accessions, cultivated faba bean accessions and Portuguese accessions) no population structure was observed based on geography or climatic factors. Differences between V. narbonensis and V. johannis are undetectable although these wild taxa are clearly distinct from V. faba accessions. Thus, a limited gene flow occurred between cultivated accessions and wild relatives. Contrastingly, the lack of population structure seems to indicate a high degree of gene flow between V. faba accessions, possibly explained by the partially allogamous habit in association with frequent seed exchange/introduction.

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Genetic diversity inVicia fabaL. populations cultivated in Tunisia revealed by simple sequence repeat analysis

Cited by 8 publications

References 38 publications

Genetic diversity in some faba bean (Vicia faba L.) genotypes assessed by simple sequence repeats

Genetic diversity in some faba bean (Vicia faba L.) genotypes assessed by simple sequence repeats

Agro-Morphological Variation and Genetic Diversity Assessment of Tunisian Sunflower (Helianthus Annuus L.) Accessions Using Microsatellite Markers

Genetic Diversity and Population Structure in Vicia faba L. Landraces and Wild Related Species Assessed by Nuclear SSRs

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