Genetic Diversity in<i>Passiflora</i>Species Assessed by Morphological and ITS Sequence Analysis

Ramaiya, Shiamala Devi; Bujang, Japar Sidik; Zakaria, Muta Harah

doi:10.1155/2014/598313

Cited by 23 publications

(25 citation statements)

References 43 publications

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“…However, intra specific variation was not observed in P. edulis which was characterized by the three unique chromosomal types, A, B and C. The resemblance in the number and type of chromosomes in varieties of P. edulis revealed their close relationships. In accordance with the current study, high levels of genetic similarity and divergence was noticed in the accessions of P. edulis and P.foetida respectively [23]. RAPD analysis revealed low level of intraspecific variations in P.edulis [9].…”

Section: Discussionsupporting

confidence: 87%

Genetic diversity in two species of Passiflora L. (Passifloraceae) by karyotype and protein profiling

Beena

Beevy

2015

Nucleus

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Karyotype and SDS-PAGE analysis in three accessions each of Passiflora edulis; P. edulis Sims. f. flaviocarpa O.Deg -yellow passion fruit, P.edulis f. edulis -purple passion fruit, and P.edulis 'Panama Red', and in three varieties of the wild species, P. foetida, P.foetida var. foetida, P.foetida var. hispida, and P. foetida var. gossippifolia, were carried out to study the genetic diversity in the species. Analysis of root tip cells revealed the presence of 2n=18 in P.edulis, 2n=16 in P.foetida var. foetida, and 2n=20 in P.foetida var. hispida and P.foetida var. gossippifolia. The somatic chromosome number of two varieties viz, P.foetida var. hispida, and P. foetida var. gossippifolia are reported for the first time. Karyotype analysis and SDS-PAGE protein profiling suggest variations at the intra-as well as interspecific levels in Passiflora. Low TF% i.e., 40 % observed in varieties of P. edulis point to the evolved nature of the cultivated taxa. Unique protein bands in P. foetida (65.66KDa) and P.edulis (17.50KDa) support their interspecific variation. Dendrogram based on UPGMA analysis emphasize the relationships among the taxa.

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Section: Discussionsupporting

confidence: 87%

Genetic diversity in two species of Passiflora L. (Passifloraceae) by karyotype and protein profiling

Beena

Beevy

2015

Nucleus

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“…Both the molecular phylogeny and the morphological characters place this species in a position highly divergent from the other species (Yockteng & Nadot, 2004; Ramaiya et al . 2014). In addition, interspecific crosses, which can be used to infer genomic similarity between species, are not successful among P. foetida and any other species of the subgenus Passiflora with n = 9 (Conceição et al .…”

Section: Discussionmentioning

confidence: 99%

Origin of the cultivated passion fruit Passiflora edulis f. flavicarpa and genomic relationships among species of the subgenera Decaloba and Passiflora

Silva

Souza

2020

Plant Biol J

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Passiflora edulis f. flavicarpa is the most economically important species in the genus Passiflora. However, the origin of this yellow form of passion fruit remains unclear, being suggested as a hybrid (P. edulis f. edulis × P. ligularis) or wild mutant. Here, the origin and genomic relationships of P. edulis f. flavicarpa with some related species in the genus Passiflora (subgenera Decaloba and Passiflora) were investigated using genomic in situ hybridization (GISH). Genomic DNA of 18 species was used as probe, which was hybridized onto chromosomes of P. edulis f. flavicarpa. Of all genomic DNA probes tested, none allowed us to identify a specific chromosome set in P. edulis f. flavicarpa. Conversely, probes from the subgenus Passiflora, P. edulis f. edulis, P. alata, P. cincinnata, P. coccinea, P. nitida and P. vitifolia, produced intense and uniform hybridizations on all chromosomes of P. edulis f. flavicarpa. Moreover, probes from P. ligularis, P. foetida and P. sublanceolata produced more intense hybridizations in the terminal region of four chromosomes, corresponding to the DNAr 45S locus, and also dispersed, less intense, hybridization across all chromosomes. Probes from the subgenus Decaloba, P. biflora, P. capsularis, P. cervii, P. coriacea, P. micropetala, P. morifolia, P. rubra and P. suberosa, produced hybridizations restricted to the DNAr 45S sites. The hybrid origin of P. edulis f. flavicarpa could not be supported based on the GISH results, and it is suggested that this species is conspecific with P. edulis f. edulis, because the probe with DNA of this form hybridized strongly throughout the target genome. The other putative parent species, P. ligularis, showed only a distant relationship with the target genome. The results also suggest that species of the subgenus Passiflora share many repetitive sequences and that the relationship between subgenera Decaloba and Passiflora is very distant.

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“…Most passion fruit species are native to Central and South America. The primary biodiversity areas are located in Colombia and Brazil (Ramaiya et al, 2014).…”

Section: Notas Científicasmentioning

confidence: 99%

Pyoverdine use for the control of passion fruit bacterial blight

Ribeiro

Souza²,

Schurt³

et al. 2017

Pesq. agropec. bras.

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-The objective of this work was to evaluate the effect of a bacterial filtrate containing pyoverdine on the population dynamics of Xanthomonas axonopodis pv. passiflorae, and the severity of passion fruit bacterial blight. The treatments were: King's B medium solution; King's B medium solution with 2 μmol L -1 Fe 2+ supplementation; and King's B medium filtrate containing two concentrations of the siderophore pyoverdine produced by Pseudomonas sp. The filtrate containing pyoverdine at Abs 363 = 0.231 (highest concentration) reduces the number of cells of X. axonopodis pv. passiflorae, and at Abs 363 = 0.115 and Abs = 0.231 significantly reduces the severity of bacterial blight.Index terms: Passiflora edulis, Pseudomonas, Xanthomonas axonopodis pv. passiflorae, phylloplane, siderophore. Passion fruit, Passiflora edulis, is native to Brazil and is widely cultivated for therapeutic use, cosmetic products and, particularly, for juice production. Most passion fruit species are native to Central and South America. The primary biodiversity areas are located in Colombia and Brazil (Ramaiya et al., 2014). Uso de pioverdina no controle da mancha bacteriana do maracujazeiroOne of the most important passion fruit diseases is bacterial blight, which is caused by Xanthomonas axonopodis pv. passiflorae, and represents severe economic losses to farmers (Meletti, 2011).Genetic improvement is one of the most promising methods for controlling the disease in commercial crops (Nakatani et al., 2009;Meletti, 2011); however, it is not efficient to prevent bacterial development (Munhoz et al., 2011).Therefore, biological control should be investigated. Iron is an essential micronutrient for both plants and microorganisms, including bacteria. In ironlimited environments, some bacteria can overcome iron restriction using siderophores, which are ironchelating compounds. Pyoverdines are water-soluble siderophores produced by fluorescent Pseudomonas spp. with a high affinity for iron (Expert & O'Brian, 2012), which limits iron availability to pathogens. Halfeld-Vieira et al. (2015) observed the potential of pyoverdine-producing phylloplane bacteria in disease management. However, the effect of these compounds on the population density of X. axonopodis pv. passiflorae on the phylloplane, and how this potential alteration could affect disease severity remains unknown.The objective of this work was to evaluate the effect of a bacterial filtrate, containing pyoverdine, on the populational dynamics of X. axonopodis pv. The in vivo experiment was carried out using the antagonist isolate Pseudomonas sp. 29RR, which was used in a previous study to control passion fruit bacterial blight. This antagonist strain produces pyoverdine that inhibits the Xap-SP growth by restricting iron availability in the culture medium, instead of restricting it by antibiosis (Halfeld-Vieira et al., 2015).First, the 29RR isolate was cultured in test tubes with King's B medium for 48 hours at 27°C (King et al., 1954). Three treatments (T) were performed: T1, Kin...

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Genetic Diversity inPassifloraSpecies Assessed by Morphological and ITS Sequence Analysis

Cited by 23 publications

References 43 publications

Genetic diversity in two species of Passiflora L. (Passifloraceae) by karyotype and protein profiling

Genetic diversity in two species of Passiflora L. (Passifloraceae) by karyotype and protein profiling

Origin of the cultivated passion fruit Passiflora edulis f. flavicarpa and genomic relationships among species of the subgenera Decaloba and Passiflora

Pyoverdine use for the control of passion fruit bacterial blight

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