Genetic diversity inNarcissus poëticusL. andN. radiiflorusSalisb. (Amaryllidaceae) in two different populations: AFLP and karyological studies.

Abstract

The two taxa, Narcissus poëticus and N. radiiflorus, although apparently quite morphologically distinct, grow together and there has been some debate as to whether they should be considered as a single or two separate species. In order to throw some light on this debate, a survey of amplified fragment length polymorphism (AFLP) was conducted to examine genetic diversity/similarity among 120 individuals of N. poëticus and N. radiiflorus obtained from two populations coming from sites separated by more than 100 km. In addition, a karyological study of the two taxa was carried out to determine chromosome number and morphology, the number of nucleoli and banding pattern with the fluorochromes chromomycin A 3 and DAPI. Using two AFLP primer pairs, a total of 103 clearly scorable fragments were amplified; of which 91 (88%) were polymorphic (78% if polymorphism is described as the most common band not present in more than 95% of individuals sampled). Cluster analysis and principal coordinates analysis of the calculated similarity matrix revealed there to be only a low level of genetic diversity between the entities constituting the populations and species. The genetic distance between the individual belonging to the different populations of the same species was slightly greater than that between species. Comparative chromosome analysis showed no differences in the two taxa at the cytological level.

Genetic variation within three populations of Phycella australis (Phil.) Ravenna from Biobío Region, Chile, evaluated using ISSR markers

“…The polymorphism percentage detected in P. australis is within the range previously founded for other Amaryllidaceae species such as Narcissus poeticus L. and N. radiiflorus Salisb. (88%, Tucci et al, 2004) and in L. longituba (65.96%, Deng et al, 2006b), despite of the fact that this studies have been performed with others types of molecular markers (AFLP and RAPD, respectively). Hamrick and Godt (1996) agree that plants with different reproductive methods, seed dispersion mechanisms, geographic distribution, and life forms tend to maintain different levels of genetic variability within populations.…”

Effect of radiation on regeneration of Chinese narcissus and analysis of genetic variation with AFLP and RAPD markers

“…AFLP is considered to be more effective than RAPD in discrimination genetic diversity (Powell et al 1996), and detection genetic variation induced by chemical and physical mutagens (Patzak 2003;Saker et al 2006). Several DNA marker techniques have been used to investigate genetic relationships among accessions in narcissus (Tucci et al 2004). …”

Genetic Diversity Analysis Based on SSR Markers in Daffodils (Narcissus)

“…Similarly because of its long generation time the relatively high genetic variation of most populations of Narcissus could reflect the genetic diversity of formally much larger population, this could explain the weak relation between genetic variability and current population size. Jaccard's similarity data a UPGMA based dendrogram was established showing molecular diversity in daffodils, same procedure was also utilized in daffodils by Tucci et al, (2004) and Nunez et al, (2003). High proportion of polymorphic loci and mean number of allele per locus occurring within population suggest that these have not experienced severe or long lasting population bottlenecks causing loss of genetic diversity.…”