Genetic diversity in <i>Fusarium oxysporum</i> f. sp. <i>melonis</i>*

Veloso, Manuela; Melo, Eduardo Madruga; Jorge-Silva, Maria L.; Bravo, M. A.

doi:10.1111/j.1365-2338.2000.tb00878.x

Cited by 4 publications

(3 citation statements)

References 7 publications

(6 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Race 1,2 overcomes these two resistance genes and is further divided into pathotype 1,2y, which induces leaf yellowing symptoms before the death of the plants, and 1,2w, which causes wilting and death without prior yellowing symptoms (Bouhot 1981). More recently, race 1,2 has spread through the world (Veloso et al 2000;Perchepied and Pitrat 2004;Herman and Perl-Treves 2007). The spread of this race has become a problem for melon cultivation, and the development of genotypes with resistance to multiple races of the pathogen represents a major objective in melon breeding programs.…”

Section: Species Races and Vegetative Compatibility Groupsmentioning

confidence: 99%

Breeding melon for resistance to Fusarium wilt: recent developments

et al. 2013

View full text Add to dashboard Cite

Melon Fusarium wilt (MFW), caused by Fusarium oxysporum f. sp. melonis (Fom), is one of the most destructive diseases of melon (Cucumis melo L.). The development and deployment of resistant cultivars is generally considered to be the best approach to control MFW. Based on the host resistance genes associated with variants of this pathogen, Fom isolates were classified into four physiological races designated 0, 1, 2, and 1,2. Two dominant resistance genes, Fom-1 and Fom-2, control resistance to races 0 and 2, and 0 and 1, respectively. Fom isolates classified as race 1,2 are able to induce disease in melon lines carrying the above resistance genes. Many sources of resistance to Fom races 0, 1, and 2 have been reported. Partial resistance to race 1,2 controlled by polygenic recessive genes was only detected in a few Far Eastern melon accessions, except for the breeding line BIZ where complete resistance was described. Identification of DNA markers tightly linked to genes conferring resistance to Fom has immediate application in MFW resistance breeding programs. The Fom-2 gene has been cloned, and it encodes a protein with a nucleotide binding site (NBS) and leucine-rich repeats domain (LRR). Based on the sequence of this domain, some molecular markers linked to this gene were developed. Several DNA markers linked to Fom-1 have also been described. However, the usefulness of these markers was variety-dependent. Therefore, their combined use would be very useful in marker assisted selection for introducing resistance to Fom races 0 and 2 in melon. Recently, these markers were used for the positional cloning of this gene, which encoded a protein with a NBS–LRR domains that shows similarity to the toll and interleukin-1 receptores (TIR). Regarding Fom race 1,2, nine QTL were detected on five linkage groups by composite interval mapping. In this paper we review the current knowledge of MFW disease, and focus on genetic resistance to Fom and marker-assisted selection for resistance.

show abstract

Section: Species Races and Vegetative Compatibility Groupsmentioning

confidence: 99%

Breeding melon for resistance to Fusarium wilt: recent developments

et al. 2013

View full text Add to dashboard Cite

show abstract

“…Previous attempts to evaluate FOM genetic variability by RAPD were carried out on a very small number of isolates by Veloso et al. () and by Riveros et al. ().…”

Section: Introductionmentioning

confidence: 99%

“…radicis-cucumerinum (Vakalounakis et al 2005), and to generate a race-specific SCAR markers for FOM race 2 (Luongo et al 2012). Previous attempts to evaluate FOM genetic variability by RAPD were carried out on a very small number of isolates by Veloso et al (2000) and by Riveros et al (2001). Moreover, genes containing conserved sequences, including at least one intron, such as translation elongation factor (TEF-1a), btubulin and calmodulin have been considered useful markers for species-level phylogenetics in fungi (Geiser et al 2004).…”

Section: Introductionmentioning

confidence: 99%

Genetic Diversity and Pathogenicity of Fusarium oxysporum f.sp. melonis Races from Different Areas of Italy

Luongo

Ferrarini

Haegi

et al. 2014

Journal of Phytopathology

View full text Add to dashboard Cite

Fusarium wilt caused by Fusarium oxysporum f.sp. melonis (FOM) is a devastating disease of melon worldwide. Pathogenicity tests performed with F. oxysporum isolates obtained from Italian melon‐growing areas allowed to identify thirty‐four FOM isolates and the presence of all four races. The aims of this work were to examine genetic relatedness among FOM isolates by race determination and to perform phylogenetic analyses of identified FOM races including also other formae speciales of F. oxysporum of cucurbits. Results showed that FOM race 1,2 was the most numerous with a total of eighteen isolates, while six and nine isolates were identified as race 0 and 1, respectively, and just one isolate was assigned to race 2. Phylogenetic analysis was performed by random amplified polymorphic DNA (RAPD) profiling and by translation elongation factor‐1α (TEF‐1α) sequencing. The analysis of RAPD profiles separated FOM races into two distinct clades. Clade 1, which included races 0, 1 and 1,2, was further divided into ‘subclade a’ which grouped almost all race 1,2 isolates, and into ‘subclade b’ which included race 0 and 1 isolates. Clade 2 comprised only race 2 isolates. The phylogenetic analysis based on TEF‐1α separated FOM from the other formae speciales of F. oxysporum. Also with TEF‐1α analysis, FOM races 0, 1 and 1,2 isolates grouped in one single clade clearly separated from FOM race 2 isolates which grouped closer to F. oxysporum f.sp. cucumerinum. RAPD technique was more effective than TEF‐1α in differentiating FOM race 1,2 isolates from those belonging to the closely related races 0 and 1. Both phylogenetic analyses supported the close relationship between the three different FOM races which might imply the derivation from one another and the different origin of FOM race 2.

show abstract