Genetic Diversity in Hexaploid and Tetraploid Wheat Genotypes Using Microsatellite Markers

Uddin, MM; Boerner, A.

doi:10.3329/ptcb.v18i1.3267

Cited by 20 publications

(13 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The PIC values recorded in this study are significantly higher than the PIC values reported in the other studies of Roder et al (1995) and Plaschke et al (1995). Uddin and Boerner (2008) also found similar observations of high PIC. The markers showed an average relatively high PICvalues which confirm that SSR markers used in this Fig.…”

Section: Molecular Screening For Drought Tolerance Wheat Genotypessupporting

confidence: 81%

Screening for drought tolerance in wheat genotypes by morphological and SSR markers

Woo

2020

J. Crop Sci. Biotechnol.

View full text Add to dashboard Cite

Drought stress has become a significant abiotic stress for wheat in Bangladesh. This study aimed to explore drought tolerance in Bangladeshi wheat based on morpho-physiological and molecular traits. Ten wheat genotypes selected based on the previous research were grown in pots under control (100% field capacity) and drought stress (30% field capacity), applied from third leaf stage till maturity. Drought stress affected the yield contributing characters and significantly reduced grain yield. Drought susceptibility index and drought tolerance efficiency were used as criteria for selection. Sonalika, Sourav, and BARI gom-28 were least affected and emerged as drought tolerant. Durum, Pavon-76, BARI gom-25, and BARI gom-33 were moderately tolerant, while BARI gom-24, BINA gom-1, and BARI gom-20 were susceptible as they were severely affected. Thirteen SSR markers linked to drought tolerance were used to characterize 26 wheat genotypes. A total of 73 alleles were detected with an average of 5.62 alleles per locus. Polymorphism information content (PIC) and Nei's (Proc Natl Acad Sci USA 70:3321-3323; 1973) gene diversity were 0.69 and 0.73, respectively. The coefficient of genetic similarity matrix for all 325 pairs of genotypes ranged from 0.15 to 1.00 with an average of 0.57. Similarity indices-based cluster analysis separated 26 genotypes into different groups. Two tolerant genotypes, Sourav and Sonalika, and two moderately tolerant genotypes, Durum and Pavon-76, as identified by morphological study were grouped in cluster 1. These four genotypes, therefore, passed the screening for drought tolerance in molecular and morphological studies and are promising for cultivation in the drought-prone areas of Bangladesh.

show abstract

Section: Molecular Screening For Drought Tolerance Wheat Genotypessupporting

confidence: 81%

Screening for drought tolerance in wheat genotypes by morphological and SSR markers

Woo

2020

J. Crop Sci. Biotechnol.

View full text Add to dashboard Cite

show abstract

“…The model-based population structure analysis is one of the choicest tools for the genetic categorization of any plant species including the perennial fruit tree species [ 86 ], which could be successfully utilized in conservation and optimization of the collected germplasm [ 87 ]. In the recent past, model-based population structure analysis was used for genetic differentiation of the guava germplasm.…”

Section: Discussionmentioning

confidence: 99%

Development of novel g-SSR markers in guava (Psidium guajava L.) cv. Allahabad Safeda and their application in genetic diversity, population structure and cross species transferability studies

Kumar

Singh

et al. 2020

PLoS ONE

View full text Add to dashboard Cite

Dearth of genomic resources particularly, microsatellite markers in nutritionally and commercially important fruit crop, guava necessitate the development of the novel genomic SSR markers through the library enrichment techniques. Three types of 3’ -biotinylated oligonucleotide probes [(CT) 14 , (GT) 12 , and (AAC) 8 ] were used to develop microsatellite enriched libraries. A total of 153 transformed colonies were screened of which 111 positive colonies were subjected for Sanger sequencing. The clones having more than five motif repeats were selected for primer designing and a total of 38 novel genomic simple sequence repeats could be identified. The g-SSRs had the motif groups ranging from monomer to pentamer out of which dimer group occurred the most (89.47%). Out of 38 g-SSRs markers developed, 26 were found polymorphic, which showed substantial genetic diversity among the guava genotypes including wild species. The average number of alleles per locus, major allele frequency, gene diversity, expected heterozygosity and polymorphic information content of 26 SSRs were 3.46, 0.56, 0.53, 0.29 and 0.46, respectively. The rate of cross-species transferability of the developed g-SSR loci varied from 38.46 to 80.77% among the studied wild Psidium species. Generation of N-J tree based on 26 SSRs grouped the 40 guava genotypes into six clades with two out-groups, the wild guava species showed genetic distinctness from cultivated genotypes. Furthermore, population structure analysis grouped the guava genotypes into three genetic groups, which were partly supported by PCoA and N-J tree. Further, AMOVA and PCoA deciphered high genetic diversity among the present set of guava genotypes including wild species. Thus, the developed novel g-SSRs were found efficient and informative for diversity and population structure analyses of the guava genotypes. These developed novel g-SSR loci would add to the new genomic resource in guava, which may be utilized in genomic-assisted guava breeding.

show abstract

“…Genetic diversity analysis of wheat landraces might permit to precise their classification and confirm their origin. The estimated genetic diversity of wheat landraces has great importance for optimal utilization of germplasm for plant breeding and other activities [9]. So, it is necessary to study the genetic diversity in wheat landraces in order to broaden the genetic variation of available germplasm for future breeding and conservation programs [10].…”

Section: Discussionmentioning

confidence: 99%

Molecular Assessment of Genetic Diversity among Egyptian Landraces of Wheat (Triticum aestivum L.) Using Microsatellite Markers

Al-Naggar

El-Shafi

El-Shal³

et al. 2020

AJBGMB

View full text Add to dashboard Cite

To increase the genetic progress in wheat (Triticum aestivum L.) yield, breeders search for germplasm of high genetic diversity, one of them is the landraces. The present study aimed at evaluating genetic diversity of 20 Egyptian wheat landraces and two cultivars using microsatellite markers (SSRs). Ten SSR markers amplified a total of 27 alleles in the set of 22 wheat accessions, of which 23 alleles (85.2%) were polymorphic. The majority of the markers showed high polymorphism information content (PIC) values (0.67-0.94), indicating the diverse nature of the wheat accessions and/or highly informative SSR markers used in this study. The genotyping data of the SSR markers were used to assess genetic variation in the wheat accessions by dendrogram. The highest genetic distance was found between G21 (Sakha 64; an Egyptian cultivar) and the landrace accession No. 9120 (G11). These two genotypes could be used as parents in a hybridization program followed by selection in the segregating generations, to identify some transgressive segregates of higher grain yield than both parents. The clustering assigned the wheat genotypes into four groups based on SSR markers. The results showed that the studied SSR markers, provided sufficient polymorphism and reproducible fingerprinting profiles for evaluating genetic diversity of wheat landraces. The analyzed wheat landraces showed a good level of genetic diversity at the molecular level. Molecular variation evaluated in this study of wheat landraces can be useful in traditional and molecular breeding programs.

show abstract

Genetic Diversity in Hexaploid and Tetraploid Wheat Genotypes Using Microsatellite Markers

Cited by 20 publications

References 24 publications

Screening for drought tolerance in wheat genotypes by morphological and SSR markers

Screening for drought tolerance in wheat genotypes by morphological and SSR markers

Development of novel g-SSR markers in guava (Psidium guajava L.) cv. Allahabad Safeda and their application in genetic diversity, population structure and cross species transferability studies

Molecular Assessment of Genetic Diversity among Egyptian Landraces of Wheat (Triticum aestivum L.) Using Microsatellite Markers

Contact Info

Product

Resources

About