Genetic diversity in European winter triticale determined with SSR markers and coancestry coefficient

Abstract

Knowledge of the genetic diversity of a species is important for the choice of crossing parents in line and hybrid breeding. Our objective was to investigate European winter triticale using simple sequence repeat (SSR) markers and the coancestry coefficient ( f) with regard to genetic diversity and grouping of germplasm. Three to five primer pairs for each of the 42 chromosomes were selected to analyse 128 European winter triticale varieties and breeding lines. SSR analysis resulted in the identification of 657 alleles with an average of 6.8 alleles per primer pair. The average polymorphism information content (PIC) for polymorphic markers was 0.54. Correlation between f and genetic similarity (GS) estimates based on Rogers' Distance was low (r(fxGS(ABDR))=0.33). The analysis of molecular variance (AMOVA) revealed that 84.7% of the total variation was found within breeding companies, and 15.3% among them. In conclusion, SSR markers from wheat and rye provide a powerful tool for assessing genetic diversity in triticale. Even though no distinct groups within the European winter triticale pool could be detected by principal co-ordinate analysis, this study provides basic information about the genetic relationships for breeding purposes.

Identification of hybrids between triticale and Aegilops juvenalis (Thell.) Eig and determination of genetic similarity with ISSRs

“…On the other hand, Sarla et al (2005) with ISSRs and Li et al (2000) with SSRs determined PIC values similar to ours: 0.50 for a group of rice cultivars and 0.57 and 0.51 for Avena species and cultivars, respectively. Among winter triticale cultivars and breeding lines, the average PIC calculated on the basis of polymorphic SSR markers was also similar to the one we obtained in our study (0.54) (Tams et al, 2004). In Brazilian triticale, PIC of SSRs was calculated at 0.36, indicating low variability in the set of 54 genotypes studied (Da Costa et al, 2007).…”

Genetic diversity of Brazilian triticales evaluated with genomic wheat microsatellites

“…Molecular markers such as microsatellites or SSRs (Simple Sequence Repeats) constitute an important tool for studies on genetic diversity, population structure, genetic mapping and crop breeding due to their abundance, codominance, level of polymorphism, reliability and easiness to assay (Röder et al, 1995(Röder et al, , 1998 Microsatellites of wheat or rye have been efficiently employed in analysis of the genome of triticale (Kuleung et al, 2004(Kuleung et al, , 2006Tams et al, 2004) and the estimates revealed on the genetic diversity of triticales from distinct continents indicate that the genetic base of this cereal must be extended (Darvey, 1986, Nascimento Junior et al, 2004. Analyzing the genome of five triticales from Russia, Mexico and the United States, using 176 microsatellites from wheat (148) and rye (28), Kuleung et al (2004) identified polymorphism in 31% of the used markers.…”

“…Analyzing the genome of five triticales from Russia, Mexico and the United States, using 176 microsatellites from wheat (148) and rye (28), Kuleung et al (2004) identified polymorphism in 31% of the used markers. Tams et al (2004) determined polymorphism information content (PIC) of 0.54, indicative of moderate variability, in the evaluation of 128 triticale cultivars from five continents using three to five microsatellites per chromosome. The same value was also found by Kuleung et al (2006), when 80 hexaploid triticales were analysed, employing 43 wheat and 14 rye microsatellites.…”

Evaluasi 18 primer SSR untuk pengembangan sidikjari DNA tanaman karet (Hevea brasiliensis Muell. Arg.) Evaluation of 18 SSR primers to develop DNA fingerprint of rubber tree (Hevea brasiliensis Muell. Arg.)

“…Salah satu penanda molekuler berbasis PCR yang sangat popular saat ini adalah Simple sequence repeat (SSR). Penanda ini telah digunakan untuk mempelajari keragaman genetik pada berbagai tanaman, seperti gandum (Liu et al, 2007;Hao et al, 2006), barley (Brantestam et al, 2007), cole (Hasan et al, 2006), jowar (Marco et al, 2007), winter triticale (Tams et al, 2004), dan kopi (Aggarwal et al, 2007). Selain relatif sederhana dan murah, teknik ini juga lebih reproduksibel, bersifat kodominan dan multi alelik, melingkupi daerah genom yang luas, serta pada umumnya bersifat polimorfik (Saha et al, 2005;Gouvêa et al, 2010).…”