Genetic Diversity in Cowpea [Vigna unguiculata (L.) Walp.] Accessions using Protein Profiling

Sharma, Divya Bhanu

doi:10.18782/2320-7051.2768

Cited by 15 publications

(20 citation statements)

References 7 publications

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“…Among the biochemical markers, SDS-PAGE shows better validity and ease for ascertaining genetic diversity of crop species and consequently it is employed in a wide range of crops for diversity analysis 10 . The SDS-PAGE profile of seed storage proteins has been used by several researchers for the assessment of genetic variability in cowpea 11 . However, the use of SDS-PAGE for the evaluation of genetic variability in the mutagenized populations of cowpea is very meagre.…”

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confidence: 99%

Characterization of Induced High Yielding Cowpea Mutant Lines Using Physiological, Biochemical and Molecular Markers

Raina

Laskar

Tantray

et al. 2020

Sci Rep

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cowpea, Vigna unguiculata (L.) Walp. is an important grain legume grown in the dry agro-ecologies of the tropics with considerably low yield due to lack of improved varieties, aggravated by prevalent narrow genetic base. thus, induced mutagenesis was employed using sodium azide and gamma rays to increase genetic variability in cowpea genotypes that resulted in isolation of eleven high yielding mutant lines at the M 4 generation from the genetic background of cowpea varieties Gomati VU-89 and Pusa-578. In order to analyze the induced genetic divergence among the mutant lines and parent genotypes, biochemical and molecular characterization was carried out with sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), simple sequence repeat (SSR) and cAAt box derived polymorphism (cBDp) markers. Activity of nitrate reductase (nR) and content of chlorophyll, carotenoid, protein and mineral were found to be significantly high in the selected mutant lines compared to their respective parent genotypes. SDS-PAGE profile of seed proteins generated 54 and 28 polymorphic bands and a total polymorphism of 62.06 and 41.17% in Gomati VU-89 and Pusa-578, respectively. SSR primers amplified a total of 16 and 24 polymorphic bands with an average polymorphism of 20.69 and 50.74% in Gomati VU-89 and Pusa-578, respectively. CBDP markers, used for the first time in mutagenized population, generated 175 bands with 77 bands being polymorphic in Gomati VU-89 and 121 bands with 59 bands being polymorphic in Pusa-578. Physiological, biochemical and molecular profiling of the selected promising mutants lines showed that Gomati VU-89-G and Pusa-578-C are genetically most diverged high yielding genotypes with significant increase in protein and micronutrient content, therefore, could be recommended for further research considerations. thus, the favorable combination of genes induced in the novel cowpea mutants selected through the present study are valuable to correspond farmers requirements for new improved cultivars (direct or hybrids).Pulses are the chief components of the agricultural system, effectively boosting food and nutrition, revenue and environment across the globe and hence assumed ideal for acquiring food security in the developing world including India. Even though India is the leading producer of pulses with 26% of the total world production harvested at about 35% of the total world area, yet a prominent yield gap of 18% exists and one of the leading importer nation 1 . Cowpea [Vigna unguiculata (L.) Walp.] is a vital leguminous crop growing in the semi-arid tropics of Asia, Africa, Southern Europe, Southern United States, and Central and South America 2 . Cowpea leaves, green pods, and grains are excellent dietary source of protein, thus used as food and feed for healthy growth of both humans and livestock 3 . Cowpea is reportedly considered to be first originated and domesticated in Southern Africa, and later spread to east and West Africa and Asia 4 . Worldwide about 6.5 million metric tons of cowpea are produc...

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mentioning

confidence: 99%

Characterization of Induced High Yielding Cowpea Mutant Lines Using Physiological, Biochemical and Molecular Markers

Raina

Laskar

Tantray

et al. 2020

Sci Rep

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“…The parents / genotypes used in the study were different released varieties, therefore, it was thought justified to determine their differences among themselves on the basis of the profile of their seed storage proteins. Characterization of genotypes based on seed storage protein/subunits is well documented in different groups of crops such as blackgram (Ghafoor and Ahmad, 2005;Ghafoor et al, 2002), Capsicum annuum L. (Anu and Peter, 2003), Solanum (Menella et al, 1999), Vignaspp (Rao et al, 1992;Sharma, 2012;Chaoudhary, 2013) and Wheat (Siddiqui and Naz, 2009), including mustard (Rabbani et al, 2001;Geetha and Balamurugan, 2011;Mukhlesure and Hirata, 2004;Parashar, 2014).…”

Section: Resultsmentioning

confidence: 99%

Assessment of Genetic Diversity for Storage Seed Protein in a Set of Released Varieties and their Diallel Set of Crosses in Indian Mustard [Brassica juncea (L.) Czern. & Coss.] Using SDS PAGE

Jangid¹,

Krishna²,

Parashar³

et al. 2017

Int.J.Curr.Microbiol.App.Sci

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“…Diversity existing in germplasms of crops collected from diverse growing regions need to be properly characterized and evaluated to improve strategies for conservation and utilization towards cultivar development (Parashar et al, 2015;Sharma and Krishna, 2017). Several strategies have been adopted for germplasm characterization including the use of morphological markers (Kancherla and Bhalla, 2003), seed storage protein markers and DNA molecular markers (Rahman and Hirata, 2004).…”

Section: Introductionmentioning

confidence: 99%

“…Unlike morphological markers (Siddiqui and Naz, 2009), the banding patterns of protein markers are stable (Iqbal et al, 2005;Nasar et al, 2006;Iqbal et al, 2014). Due to these benefits, protein markers have been widely applied in the analysis of genetic diversity within and between accessions, in studying plant domestication in relation to genetic resource conservation and breeding, and in establishing genome relationships (Kakaei and Kahrizi, 2011;Hameed et al, 2012;Sharma and Krishna, 2017). Seed storage protein profiling has been used in investigating diversity among selected varieties of Brassica napus (Nasar et al, 2006;Choudhary et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

“…The use of sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) in studying plant proteins was found to be simple and inexpensive; hence, it has applications in the improvement of the crop of interest through breeding (Zada et al, 2013;Sharma and Krishna, 2017). However, so far, only few studies were made regarding the diversity of storage proteins in the garden cress germplasm.…”

Section: Introductionmentioning

confidence: 99%

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Genetic diversity of seed storage protein in the Ethiopian garden cress (Lepidium sativum L.)

Tadesse¹,

Mekbib²,

Wakjira³

et al. 2018

Afr. J. Biotechnol.

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The Ethiopian garden cress (Lepidium sativum L.) is an important crop extensively used as food and medicine. In this study, total seed storage proteins of 112 garden cress genotypes collected from diverse growing regions in Ethiopia were investigated to assess patterns of genetic diversity and relationships. Using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), a total of 1774 stable protein bands were identified through discontinuous electrophoresis. Of these, 1597 bands were polymorphic. A maximum of 20 protein sub-units in the range of 15 to 75 kDa were observed per genotype. The similarity coefficient among these genotypes ranged from 0.25 to 1.00 with an average genetic dissimilarity of 0.2754. On the basis of Ward Euclidian distance, the genotypes were grouped into five major clusters, the largest one comprised of 62 genotypes (Cluster III) followed by 26 genotypes in Cluster I. Cluster IV and V contained a total of 14 genotypes that were the most distantly related to other groups, and thus can be potentially used as parents for exploitation of heterotic effects in hybrid breeding programs. Our findings using SDS-PAGE profiles revealed no obvious association between geographic region of origin and germplasm clustering. However, the polymorphism and cluster analysis indicated that garden cress genotypes differed greatly in the composition of seed proteins. This shows that protein profiling could be used as a rapid and reliable method for genetic diversity studies. In order to fully explore the protein based genetic diversity in garden cress germplasm, techniques such as 2-D gel electrophoresis are recommended in future studies.

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Genetic Diversity in Cowpea [Vigna unguiculata (L.) Walp.] Accessions using Protein Profiling

Cited by 15 publications

References 7 publications

Characterization of Induced High Yielding Cowpea Mutant Lines Using Physiological, Biochemical and Molecular Markers

Characterization of Induced High Yielding Cowpea Mutant Lines Using Physiological, Biochemical and Molecular Markers

Assessment of Genetic Diversity for Storage Seed Protein in a Set of Released Varieties and their Diallel Set of Crosses in Indian Mustard [Brassica juncea (L.) Czern. & Coss.] Using SDS PAGE

Genetic diversity of seed storage protein in the Ethiopian garden cress (Lepidium sativum L.)

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