Genetic Diversity in California and Florida Populations of the Pitch Canker Fungus<i>Fusarium subglutinans</i>f. sp.<i>pini</i>

Correll, J. C.

doi:10.1094/phyto-82-415

Cited by 81 publications

(71 citation statements)

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“…These included single-conidium isolates from 69 P. patula seedlings in a forest nursery in South Africa collected during June 1990 (19 isolates), November 1990 (16 isolates), June 1991 (19 isolates) and August 1991 (15 isolates), and isolates recovered from pitch canker-infected trees in Florida (19) and California (five). The isolates from Florida and California were representative of the major VCGs that occur in those areas (Correll et al, 1992). All isolates are maintained in cryovials in 15% glycerol at ¹70ЊC (Department of Microbiology and Biochemistry, University of the Free State, Bloemfontein, South Africa).…”

Section: Isolatesmentioning

confidence: 99%

“…The teleomorph of F.s. pini has not been observed in nature and is rare in culture (Kuhlman et al, 1978;Correll et al, 1992). The genetic diversity of F.s.…”

Section: Introductionmentioning

confidence: 99%

“…Until recently, Fusarium species have not been widely used in genetic studies of variation among and within populations (Burnett, 1984). This situation has changed over the last 10 years, and many species have been subjected to genetic analysis (Van Etten & Kistler 1988;Kistler & Momol, 1990;Leslie, 1990Leslie, , 1993Correll et al, 1992). Characters such as heterokaryon formation between isolates of Fusarium are often used in studies of self/non-self recognition.…”

Section: Introductionmentioning

confidence: 99%

“…The genetic diversity of F.s. pini has been studied in Florida, where pitch canker is well established, and in California, where the disease was reported for the first time in 1986 (Correll et al, 1992;Gordon et al, 1996).…”

Section: Introductionmentioning

confidence: 99%

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Genotypic diversity in a South African population of the pitch canker fungus Fusarium subglutinans f.sp. pini

et al. 1997

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The genotypic diversity in a South African population of Fusarium subglutinans f.sp. pini (F.s. pini) was determined, based on the number of vegetative compatibility groups (VCGs). Isolates of F.s. pini from South Africa (69), California (five) and Florida (19) were included in the study. The nit1 (or nit3) and NitM mutants were selected as chlorate resistant sectors and paired on minimal medium. The South African isolates of F.s. pini were assigned to 23 different VCGs. No heterokaryons formed between isolates from South Africa, California and Florida. The high degree of genotypic diversity in the South African population of F.s. pini is probably due to some level of sexual reproduction in the population.

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Section: Isolatesmentioning

confidence: 99%

“…The teleomorph of F.s. pini has not been observed in nature and is rare in culture (Kuhlman et al, 1978;Correll et al, 1992). The genetic diversity of F.s.…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Genotypic diversity in a South African population of the pitch canker fungus Fusarium subglutinans f.sp. pini

et al. 1997

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“…Entre as técnicas moleculares utilizadas para discriminação de Fusarium spp. destacam-se: Polimorfismo de DNA amplificado ao acaso (RAPD) (Duteau & Leslie, 1991;Ouellet & Seifert, 1993;Voigt et al, 1995;Viljoen et al, 1997), Polimorfismo no comprimento de fragmentos de restrição (RFLP) (Correll et al, 1992;Nicholson et al, 1993), espaços intergênicos transcritos (ITS 1 e ITS 2) (O'Donnell & Cigelnik, 1997;Waalwijk et al, 1996) e dados de seqüências gênicas conservadas (Steenkamp et al, 2000a), Polimorfismo no comprimento de fragmentos amplificados (AFLP) (Jurgenson et al, 2002) e Reação de Polimerase em Cadeia (PCR) (Hue et al, 1999;Steenkamp et al, 2000b). Steenpkamp et al (1999) combinaram as técnicas de PCR-RFLP para a diferenciação dos fungos de F. subglutinans associados a vários hospedeiros (cana-de-açúcar, pinheiro, mangueira e milho).…”

Section: Introductionunclassified

Utilização de marcador molecular SCAR na identificação de Fusarium subglutinans, agente causal da malformação da mangueira

et al. 2007

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RESUMO-O gênero Fusarium é responsável por doenças em diversas plantas economicamente importantes. Entre estas doenças, destaca-se a malformação da mangueira, causada pelo fungo Fusarium subglutinans. O objetivo deste trabalho foi desenvolver oligonucleotídeos iniciadores para reação em cadeia da polimerase (PCR), específicos para o fungo F. subglutinans da mangueira. A amplificação de DNA de oito Fusarium spp. de diferentes hospedeiros, usando o oligonucleotídeo randômico UBC-41 (TTAACCGGGG), produziu um fragmento de aproximadamente 1.300 pb somente para o fungo da mangueira. Tendo em vista que padrões de bandeamento por RAPD não são considerados confiáveis devido à baixa reprodutibilidade dos resultados, o fragmento diferencial foi eluído do gel de agarose, purificado, clonado e seqüenciado. As seqüências nucleotídicas foram utilizadas para identificar e sintetizar quatro pares de oligonucleotídeos específicos, denominados Fs 5, Fs 13, Fs 14 e Fs 15. DNAs de Fusarium spp. de outros hospedeiros (alho, amendoim, cana-de-açúcar, ciclâmen, ervilha, melão e trigo), da planta de mangueira cv. Tommy Atkins sadia e de outros cinco isolados de F. subglutinans de mangueira sintomática, foram submetidos à amplificação com os pares de oligonucleotídeos. Fragmentos amplificados foram visualizados somente para F. subglutinans de mangueira, demonstrando assim a especificidade dos oligonucleotídeos SCAR desenhados. Termos de Indexação: oligonucleotídeos iniciadores, Mangifera indica, marcadores moleculares, RAPD-PCR. USE OF MOLECULAR MARKER SCAR IN THE IDENTIFICATION OF Fusarium subglutinans,CAUSAL AGENT OF MANGO MALFORMATIONABSTRACT-The Fusarium genus is responsible for serious diseases in many economically important crops. Among these diseases it is distinguished the mango flower malformation, caused by the fungus Fusarium subglutinans. The objective of this work was to develop specific oligonucleotide primers for the polymerase chain reaction (PCR) targeting the mango flower malformation by fungus F. subglutinans. The DNA amplification of eight Fusarium spp. collected from different hosts making the use of a random oligonucleotide primer UBC-41 generated a fragment of approximately 1300 pb in size, specifically for the mango flower malformation fungus (Fusarium subglutinans). Since standard RAPD banding patterns are not considered reliable because of their low results of reproducibility, the distinctive fragment was eluted off the agarose gel, purified, cloned and then sequenced. The nucleotide sequences were used to identify and also to synthesize four pairs of specific oligonucleotide named herein Fs 5, Fs 13, Fs 14 and Fs 15. Other Fusarium spp. DNAs sampled from other hosts (garlic, peanut, sugarcane, cyclamen, pea, melon and wheat), from a healthy mango tree cv. Tommy Atkins and other six isolates from symptomatic mango plants were submitted to PCR amplification with these pairs of oligonucleotide. Only fragments from the mango tree fungus Fusarium subglutinans were visualized, showing this way the SCAR oligonucleotid...

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References

2006

The Fusarium Laboratory Manual

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Genetic Diversity in California and Florida Populations of the Pitch Canker FungusFusarium subglutinansf. sp.pini

Cited by 81 publications

References 0 publications

Genotypic diversity in a South African population of the pitch canker fungus Fusarium subglutinans f.sp. pini

Genotypic diversity in a South African population of the pitch canker fungus Fusarium subglutinans f.sp. pini

Utilização de marcador molecular SCAR na identificação de Fusarium subglutinans, agente causal da malformação da mangueira

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