Genetic Diversity in Cactus Clones Using Issr Markers

Alves, Tereza; Silva, M. V. da; Almeida, Clébia Maria Alves de; Amaral, Daniel Oliveira Jordão do; Santos, Djalma Cordeiro dos; Farias, Izeni Pires; Donato, Virgínia Maria Tenório Sabino; Costa, Andrea; Campos, F. A. P.; Júnior, José Carlos Batista Dubeux; Silva, S. de Melo

doi:10.17660/actahortic.2009.811.3

Cited by 12 publications

(5 citation statements)

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“…Different studies based on molecular markers have suggested that the current taxonomy of many species of Opuntia is erroneous (Luna-Paez et al, 2007;Zoghlami et al, 2007;Helsen et al, 2009;Souto-Alves et al, 2009;Caruso et al, 2010;Majure et al, 2012). However, the question remains whether the phenotypic differences observed in this group of plants are caused by crosspollination, adaptive genetic response to environmental differences, phenotypic plasticity, epigenetic bases, or by somatic mutations occurring in time and space resulting of the wide genomic variation observed.…”

Section: Discussionmentioning

confidence: 99%

Molecular based assessment of genetic diversity of xoconostle accessions (Opuntia spp.)

Valadez-Moctezuma¹,

Ortiz-Vásquez²,

Samah³

2014

Afr. J. Biotechnol.

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Xoconostle or acidic prickly pear is an important fruit in Mexico; it is produced by a group of Opuntia plants known for their nutritional qualities and adapted to harsh environmental conditions. In this study, we report for the first time the estimation of genetic diversity within a set of 24 xoconostle accessions using inter simple sequence repeats (ISSR) markers. Five ISSR primers were selected to detect DNA polymorphisms in these plants. Broad genetic diversity was detected (0.41-0.82, Dice coefficient) and the unweighted pair group method with the arithmetic averaging (UPGMA) analysis allowed discriminating all genotypes and enabled their clustering into six groups; the 'Matizado' accession was significantly divergent from all tested genotypes. In addition, as shown by the clustering analysis and principal component analysis (PCA), the tested genotypes did not group according to their origin, to culture conditions, or to the current species classification. This work, on one hand, demonstrates the high genetic variation among genotypes of xoconostles, and on the other hand, it suggests a taxonomic revision and raises doubts about the number of species reported for these plants.

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Section: Discussionmentioning

confidence: 99%

Molecular based assessment of genetic diversity of xoconostle accessions (Opuntia spp.)

Valadez-Moctezuma¹,

Ortiz-Vásquez²,

Samah³

2014

Afr. J. Biotechnol.

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“…In this study, 10 ISSR primers that produced high polymorphism were used to estimate the genetic variability of 60 samples. These primers present di- and tri-repeat motifs (Table 2), previously described by Souto Alves et al [64] that were used to check the diversity of a wide range of forest species. For example, Cassia fistula L. [24], Murraya koenigii [65], Apterosperma oblata [66].…”

Section: Methodsmentioning

confidence: 99%

Genetic, Morphological, and Biochemical Diversity of Argan Tree (Argania spinosa L.) (Sapotaceae) in Tunisia

Louati

Uçarlı

Arıkan

et al. 2019

Plants

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Argan trees are normally endemic to Morocco and Algeria, but hundreds of argan trees exist in Tunisia, some introduced from Morocco and some from unknown origins. The aim of the present study was to evaluate the genetic, morphological, and biochemical diversity of the argan trees in Tunisia. In this study, we used morphometric data collected from vegetative tissue, as well as pomological characteristics related to fruits, stones, and kernels. Genetic variation in 60 trees of Tunisian Argania spinosa L. was estimated using inter-simple sequence repeats (ISSRs). Mutation screening and genotyping by high-resolution melting (HRM) was performed to detect delta-6-desaturase (D6D) variants in the tested individuals, and finally fatty acid analysis of argan leaves with gas chromatography (GC) was performed. The plant materials used in this study originated from four different sites in Tunisia. Analysis of morphological characteristics showed large variability both within and between the studied collections. The analysis of ISSR polymorphisms gave information about the diversity within and between populations. HRM analysis showed that all 60 argan individuals were grouped into 10 different categories. The results of the gas chromatography analysis showed that the presence of omega-3 fatty acids EPA and DHA was noticeable in some argan leaves.

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“…In this study, ten ISSR primers that produced a clear, reproducible and relatively high polymorphism were used to estimate the genetic diversity of 200 samples (Table 2). These primers, previously described by Souto Alves et al (2009), were successfully used to study a wide range of forest species (e.g., Apterosperma oblata (Su et al, 2008), Murraya koenigii (Verma and Rana, 2011).…”

Section: Issr Pcr Amplificationmentioning

confidence: 99%

Patterns of Genetic Diversity and Structure at Fine Scale of an Endangered Moroccan Endemic Tree (<i>Argania spinosa</i> L. Skeels) Based on ISSR Polymorphism

Mouhaddab

Aabd

Achtak

et al. 2015

Not Bot Horti Agrobo

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The preservation of the diversity of endangered populations of argan trees, in their natural habitat, is a crucial step toward their conservation. The aim of the present study was to evaluate the genetic diversity of the argan trees in the wild, and to establish a phylogenetic map using DNA fingerprints. The ultimate goal was to develop a core set that would represent the existing diversity in the whole germplasm. In regard to this, 200 samples of Argania spinosa individual trees were collected from 10 different provenances in the region of Essaouira (Morocco). The genetic variation between and within these argan trees was investigated using previously described Inter-Simple Sequence Repeat markers. These markers generated a total of 149 fragments, in which 148 (99.33%) were polymorphic. The samples collected in the â€˜Ouled Lhajâ€™ provenance showed the lowest diversity (% of polymorphic locus P=48.32%; genetic diversity Nei h=0.153; allelic richness A=1.483), compared to those collected in the â€˜Mramerâ€™ provenance (%P=68.46%; h=0.233; A=1.685). Also, the results showed a high level of genetic differentiation among provenances (AMOVA=44%, Gst=0.40), and a limited gene flow (Nm=0.73) between the provenances. In addition, these data suggested a low correlation between the genetic diversity of the tree and their respective geographical location in relation to the proximity to the littoral. Finally, a core collection of 13 genotypes that represent the essential of the detected diversity was established. The distribution pattern of this genetic diversity provides an important baseline data for the conservation strategies of argan tree species in the wild.

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Genetic Diversity in Cactus Clones Using Issr Markers

Cited by 12 publications

References 0 publications

Molecular based assessment of genetic diversity of xoconostle accessions (Opuntia spp.)

Molecular based assessment of genetic diversity of xoconostle accessions (Opuntia spp.)

Genetic, Morphological, and Biochemical Diversity of Argan Tree (Argania spinosa L.) (Sapotaceae) in Tunisia

Patterns of Genetic Diversity and Structure at Fine Scale of an Endangered Moroccan Endemic Tree (<i>Argania spinosa</i> L. Skeels) Based on ISSR Polymorphism

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