Genetic Diversity in a Core Collection Established from the Main Bean Genebank in Spain

Abstract

Common bean (Phaseolus vulgaris L.) is a traditional crop in many Spanish regions. A major collection of Spanish common bean landraces is maintained at the National Genebank in the Center for Plant Genetic Resources (CRF), Alcala de Henares, Madrid, Spain. A core collection including 200 common bean accessions was established from the CRF collection. We sought to analyze the genetic diversity of this CRF core collection using morpho‐agronomic traits, phaseolin seed protein, and a set of 11 molecular markers. Accessions were classified in 65 groups according to their seed phenotype. Seventy‐one accessions have appropriate qualities for culinary use as green or snap beans. The four bean growth habits were present among the accessions included in the CRF core collection, with the indeterminate climbing habit (Type IV; 113 accessions) being the most common. Five different phaseolin patterns were found, the most common being type C (86 accessions), followed by types T (59 accessions) and S (42 accessions). With the set of molecular markers used, an average number of 6.18 alleles marker−1 and an average polymorphism information content marker−1 of 0.66 were found. The dendrogram and the principal components analysis developed using the molecular marker data revealed the existence of two main groups of accessions corresponding to the Middle American and the Andean gene pools, respectively, and suggested the existence of some intermediate forms. The possible origin of these putative intermediate forms is discussed. Knowledge of the genetic diversity in the CRF core collection will contribute to improved use and conservation of Spanish bean genetic resources.

Culinary and sensory traits diversity in the Spanish Core Collection of common beans (Phaseolus vulgaris L.)

“…To make it easier to use the material from this vast repository, a core collection was compiled based on the passport data and seed phenotype (De la Rosa et al, 2000). Later, the information about the accessions was expanded to include agronomic and morphological traits of the plants, type of phaseolin (Pérez-Vega et al, 2009), and some resistances (Pérez-Vega et al, 2006;Asensio et al, 2010;Pascual et al, 2010). 2 tivated.…”

“…2 tivated. About 75% are of Andean origin and 25% are of Mesoamerican origin (Pérez-Vega et al, 2009).…”

Determination of Phaseolin Types in Common Bean (Phaseolus vulgaris) Varieties from Turkey

“…Seeds containing Andean type phaseolin were determined to be larger compared to those containing Mesoamerican type phaseolin. Phaseolin alleles were determined to be correlated with seed size and the seed sizes were under control of a quantitative loci (Perez-Vega et al, 2009). Other than phaseolin types, the seed volumes varied among genotypes.…”

“…Bean varieties of Andean origin possessed an advantage over varieties of other origins due to better photoperiodic adaptation. Among varieties of Western Europe, type "T" phaseolin is the most dominant because of favored cultivation due to their green pods (Rodiňo et al, 2001a Researchers have utilized molecular markers to determine genetic structures and origins of bean varieties in addition to the seed storage protein "phaseolin" (Piergiovanni et al, 2000;Rodiňo et al, 2003;Logozzo et al, 2007;Limongelli et al, 1996;Perez-Vega et al, 2009;Maras and Sustar-Vozlic, 2013). Polyacrylamide gels have usually yielded the best results in determining phaseolin types.…”

Legume genetic resources: management, diversity assessment, and utilization in crop improvement

“…At both stages, standard clustering procedures are used to separate groups of similar accessions combined with various statistical tests to identify the best representatives. Core and/or mini core collections have been reported in adzuki bean, chickpea, common bean, cowpea, hyacinth bean, lentil, mungbean, pea and pigeonpea (Bisht et al 1998;Wang et al 2001;Pengelli and Maass 2001;Upadhyaya and Ortiz 2001;Upadhyaya et al , 2006Dwivedi et al 2005;Mahalakshmi et al 2007;Logozzo et al 2007;Zong et al 2008a;Pérez-Vega et al 2009;Redden et al 2009;Hamwieh et al 2009). Forming such core collection is also underway in different faba bean germplasm collections using passport and molecular data (Zong et al 2009a;Duc et al 2009).…”