Genetic diversity, forensic characteristics and phylogenetic analysis of the Qiongzhong aborigines residing in the tropical rainforests of Hainan Island via 19 autosomal STRs

Wang, Fenfen; Du, Zhengming; Han, Baozhong; Cao, Shengping; Fu, Fangshu; Luo, Zhenming; Mu, Ziqing; Chen, Jirui; Qiu, Pingming; Fan, Haoliang

doi:10.1080/03014460.2021.1951352

Cited by 4 publications

(2 citation statements)

References 53 publications

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“…The mean values and standard deviations (SDs) of the DoC, N-1 stutter ratio for Y-STRs, and ACR values for MHs were calculated by SAS ® 9.4 software (SAS Institute Inc., Cary, NC, USA). The other relevant forensic parameters were determined as described in our previous studies [88][89][90][91][92][93][94][95][96][97][98][99]. A e was calculated using the formula proposed by Kidd et al [100], A e = 1 ∑…”

Section: Forensic Parametersmentioning

confidence: 99%

Microhaplotype and Y-SNP/STR (MY): A novel MPS-based system for genotype pattern recognition in two-person DNA mixtures

Fan

Xie

Wang

et al. 2022

Forensic Science International: Genetics

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Section: Forensic Parametersmentioning

confidence: 99%

Microhaplotype and Y-SNP/STR (MY): A novel MPS-based system for genotype pattern recognition in two-person DNA mixtures

Fan

Xie

Wang

et al. 2022

Forensic Science International: Genetics

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“…Therefore, based on the underlying genome-wide data of the EAS populations from the 1,000 Genome Project (1KG) Phase 3 ( Genomes Project et al, 2010 ; Genomes Project et al, 2015 ) and the engineering fundamental logic for the maximum utilization of multiplex PCR-CE system, a novel 6-dye direct and multiplex PCR-CE-based typing system with relatively short amplicons (<230 bp), consisting of 57 autosomal InDels (A-InDels), 2 Y-chromosomal InDels (Y-InDels), and Amelogenin, was studied to expand application scenarios for forensic purposes in EAS populations, especially for different Chinese populations. Moreover, forensic efficiencies and population genetic analyses of the direct and multiplex PCR-CE-based InDel typing system were further evaluated in 26 globally dispersed populations and the Hainan Li (HNL) group, which is a relatively isolated Chinese group revealed by the previous studies ( Fan et al, 2018b ; Fan et al 2019b ; Fan et al 2021a ; Wang et al, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

Systematic Evaluation of a Novel 6-dye Direct and Multiplex PCR-CE-Based InDel Typing System for Forensic Purposes

Fan

et al. 2022

Front. Genet.

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Insertion/deletion (InDel) polymorphisms, combined desirable characteristics of both short tandem repeats (STRs) and single nucleotide polymorphisms (SNPs), are considerable potential in the fields of forensic practices and population genetics. However, most commercial InDel kits designed based on non-Asians limited extensive forensic applications in East Asian (EAS) populations. Recently, a novel 6-dye direct and multiplex PCR-CE-based typing system was designed on the basis of genome-wide EAS population data, which could amplify 60 molecular genetic markers, consisting of 57 autosomal InDels (A-InDels), 2 Y-chromosomal InDels (Y-InDels), and Amelogenin in a single PCR reaction and detect by capillary electrophoresis, simultaneously. In the present study, the DNA profiles of 279 unrelated individuals from the Hainan Li group were generated by the novel typing system. In addition, we collected two A-InDel sets to evaluate the forensic performances of the novel system in the 1,000 Genomes Project (1KG) populations and Hainan Li group. For the Universal A-InDel set (UAIS, containing 44 A-InDels) the cumulative power of discrimination (CPD) ranged from 1–1.03 × 10–14 to 1–1.27 × 10–18, and the cumulative power of exclusion (CPE) varied from 0.993634 to 0.999908 in the 1KG populations. For the East Asia-based A-InDel set (EAIS, containing 57 A-InDels) the CPD spanned from 1–1.32 × 10–23 to 1–9.42 × 10–24, and the CPE ranged from 0.999965 to 0.999997. In the Hainan Li group, the average heterozygote (He) was 0.4666 (0.2366–0.5448), and the polymorphism information content (PIC) spanned from 0.2116 to 0.3750 (mean PIC: 0.3563 ± 0.0291). In total, the CPD and CPE of 57 A-InDels were 1–1.32 × 10–23 and 0.999965, respectively. Consequently, the novel 6-dye direct and multiplex PCR-CE-based typing system could be considered as the reliable and robust tool for human identification and intercontinental population differentiation, and supplied additional information for kinship analysis in the 1KG populations and Hainan Li group.

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Development and validation of a novel 133-plex forensic STR panel (52 STRs and 81 Y-STRs) using single-end 400 bp massive parallel sequencing

et al. 2021

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Genetic diversity, forensic characteristics and phylogenetic analysis of the Qiongzhong aborigines residing in the tropical rainforests of Hainan Island via 19 autosomal STRs

Cited by 4 publications

References 53 publications

Microhaplotype and Y-SNP/STR (MY): A novel MPS-based system for genotype pattern recognition in two-person DNA mixtures

Microhaplotype and Y-SNP/STR (MY): A novel MPS-based system for genotype pattern recognition in two-person DNA mixtures

Systematic Evaluation of a Novel 6-dye Direct and Multiplex PCR-CE-Based InDel Typing System for Forensic Purposes

Development and validation of a novel 133-plex forensic STR panel (52 STRs and 81 Y-STRs) using single-end 400 bp massive parallel sequencing

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