Genetic Diversity for Barley Adaptation to Stressful Environments

Fricano, Agostino; Battaglia, Raffaella; Mica, Erica; Tondelli, Alessandro; Crosatti, Cristina; Guerra, Davide; Cattivelli, L.

doi:10.1007/978-3-030-75875-2_4

Cited by 2 publications

(2 citation statements)

References 175 publications

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“…Genetic diversity is essential to create a robust food security system capable of responding to repeated biotic and abiotic stresses [ 58 ]. In plant breeding, genetic diversity analysis is critical in identifying alleles to develop crops with high yield, resistance to biotic and abiotic stressors, and satisfactory production [ 53 ].…”

Section: Resultsmentioning

confidence: 99%

Genetic diversity and population structure of barley landraces from Southern Ethiopia’s Gumer district: Utilization for breeding and conservation

et al. 2023

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Barley is the fifth most important food crop in Ethiopia. The genetic relationship and population structure studies of barley are limited to gene bank collections. Therefore, this study fills a gap by investigating the selection, consumption, economic value, genetic diversity, and population structure of farm-collected barley from the Gumer district of the Gurage Zone, which has received little attention. The information on the use of barley in the study area was collected using semi-structured interviews and questionnaires. 124 households of 11 kebeles, the smallest community unit, were interviewed. Barley landraces collected were compared with those collected from Japan, the United States (USA), and other Ethiopian locations. Illumina iSelect (50K genotyping platform) was used to identify single nucleotide polymorphisms (SNP) (20,367). Thirty landraces were found in Gumer. Burdaenadenber had the highest on-farm Shannon index estimate (2.0), followed by Aselecha (1.97) and Enjefo (1.95). Aselecha and Fetazer had the highest (44%) and the lowest (29%) richness values, respectively. High and low Simpson index values were found in Aselecha (84%) and Wulbaragenateretero (79%), respectively. The neighbor-joining tree revealed that Gumer landraces formed a separate subcluster with a common ancestral node; a sister subcluster contained barley landraces from Japan. According to the population structure analysis, barley landraces from Gumer differed from Japan and the United States. The principal component analysis revealed that US barley was the most distant group from Gumer barley. The markers’ allele frequencies ranged from 0.10 to 0.50, with an average value of 0.28. The mean values of Nei’s gene diversity (0.38) and the polymorphic information content (0.30) indicated the presence of high genetic diversity in the samples. The clustering of accessions was not based on geographic origin. Significant genetic diversity calls for additional research and analysis of local barley diversity because the selection and use of barley in Ethiopia would have been affected by the preference of ethnic groups.

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Section: Resultsmentioning

confidence: 99%

Genetic diversity and population structure of barley landraces from Southern Ethiopia’s Gumer district: Utilization for breeding and conservation

et al. 2023

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“…The study and evaluation of molecular and genetic diversity of the barley genome, for instance, its specifi c, including microsatellite, sites, may be a relevant source of information to further use individual loci and their alleles, associated with relevant traits or their combinations for the analysis of genotypes in breeding programs. The studies on barley in this direction are still a matter of large-scale research and have been conducted for several decades already (Akar et al, 2009;Sallam et al, 2020;Fricano et al, 2021). During this period, there have been many studies on mapping microsatellites (Ramsay et al, 2000;Varshney et al, 2007) and investigation of basic collections of barley genetic material from different countries or regions based on microsatellite analysis (Zhang et al, 2014;Jo et al, 2017;Elakhdar et al, 2018;Lawati et al, 2021).…”

Section: Discussionmentioning

confidence: 99%

Variability and potential of microsatellite loci of 5HL chromosome in barley genotypes of different origin

Balvinska,

Fait

2024

Agric. sci. pract.

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Aim. To investigate the polymorphism of microsatellite loci of the 5HL chromosome of barley, including those which are location in the area of LT-resistance key genes and close to them, to determine and evaluate the frequence of microsatellite alleles in the genetic material of autumn-sown barley of different origin. Methods. The isolation of genomic DNA, microsatellite analysis, PCR amplification, gel electrophoresis, and statistical analysis. We studied 46 barley genotypes of different origin, including 33 winter barley varieties, and 13 varieties of alternative (facultative) growth habit; among these 46 barley genotypes, 21 varieties were developed by PBGI-NCSCI, 25 – of other origin, including 21 from the countries of Central Europe (Czech Republic/CZ – 2; Grabe, Luran; Germany/DE – 4: Skarpia, Majbrit, Сinderella, Highlight), Western Europe (France/FR – 1: Anzhelika; the Netherlands/NL – 1: Gerlach) and Eastern Europe (NGC named after P.P. Lukianenko/RU – 13, 11 winter varieties: Derzhavnyi, Espada, Zhavoronok, Kondrat, Kumach, Meteor, Metaksa, Mikhailo, Platon, Tigr, Khutorok, and 2 winter-and-spring varieties: Putnik, Timofei) and 4 Western-Asian varieties (Syria/SYR – 4: Pamir013/Sonata, Pamir065/Pamir149, CWB-117-77-97, ROHO). Results. The allelic polymorphism was studied by 14 microsatellite loci of the 5HL chromosome of barley, including those in the area of genes Fr-H1, Fr-H2 and close to these regions, the distribution of the identified micro- satellite alleles was studied along with their frequencies and genetic diversity in the sampling of 46 collection varieties of autumn-sown barley of different origin. Among the investigated microsatellite areas of the 5HL chromosome of barley, only 50 % of loci in the selected samples of varieties were found to be polymorphic. These were microsatellite loci Bmag0760, GMS061, Bmag0337, UMB702, Bmag0323, Bmag0223, and Bmag0222. We found the dominating alleles and those with reliably lower frequency, and the alleles specific only for some regions. The estimated values of the polymorphism information content (PIC) for the investigated polymorphic microsatellite loci varied between 0.29 (UMB702) – 0.77 (Bmag0223). The diversity index was 0.54 on average. Conclusions. The results of the study demonstrated moderate allelic diversity of the investigated microsatellite loci of the 5HL chromosome of barley, which indicates the presence of potential genetic variability of some loci, the possibility of applying them in further studies on determining the effects of specific alleles of each locus and their associations with required economically valuable traits of barley, including resistance to low temperatures (LT-resistance). The potential of polymorphic alleles as markers of frost-resistance traits of autumn-sown barley genotypes is discussed.

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Genetic Diversity for Barley Adaptation to Stressful Environments

Cited by 2 publications

References 175 publications

Genetic diversity and population structure of barley landraces from Southern Ethiopia’s Gumer district: Utilization for breeding and conservation

Genetic diversity and population structure of barley landraces from Southern Ethiopia’s Gumer district: Utilization for breeding and conservation

Variability and potential of microsatellite loci of 5HL chromosome in barley genotypes of different origin

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