Genetic diversity evaluation of winged bean (Psophocarpus tetragonolobus (L.) DC.) using inter-simple sequence repeat (ISSR)

Chen, Dongming; Yi, Xing; Yang, Hui; Zhou, Hui; Yamaguchi, Yu; Tian, Yun; Lu, Xiangyang

doi:10.1007/s10722-015-0261-3

Cited by 21 publications

(11 citation statements)

References 10 publications

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“…Principal Coordinates Analysis (PCoA) results indicated no clustering by country of origin, and the population structure suggested four subpopulations, each comprising of accessions from multiple countries, spanning in some case between South America, Asia, and Africa (Yang et al 2018). Overall, these findings were in agreement with previous genetic analysis through ISSR and RAPD markers, in which molecular markers-based grouping did not agree with geographical origins (Mohanty et al 2013;Chen et al 2015). The authors, given also the suggested presence of admixture from clusters obtained through STRU CTU RE analysis, suggested the possibility of crossing between genetic material coming from different origins, probably thanks to transport/sharing of seeds.…”

Section: The Genomics Erasupporting

confidence: 83%

“…Following on from the initial concerted research effort, winged bean research activities have expanded to include further characterisation of anti-nutritional factors in mature seeds (Umemoto et al 1992;Habu et al 1992Habu et al , 1997Mukhopadhyay 2000) as well as factors affecting plant growth and development (Schiavinato and Válio 1996a, b). And more recently, development of genetic resources such as molecular markers for genetic diversity assessment (Mohanty et al 2013;Chen et al 2015;Wong et al 2017;Yang et al 2018) or clonal fidelity (Koshy et al 2013b), and transcriptome studies (Chapman 2015;Vatanparast et al 2016;Wong et al 2017).…”

Section: Synthesis Of Past Researchmentioning

confidence: 99%

“…Cluster analysis applied to the cumulative data from the two sets of primers, grouped the 24 accessions into seven clusters that the authors judged not to be "in congruence with their geographic affiliations". Later, Chen et al (2015) worked on a different set of 45 winged bean accessions from eight countries (PNG, Indonesia, Thailand, Nigeria, Colombia, Sri Lanka, Costa Rica, and China), using a set of five ISSR markers. These latter generated 67 identifiable marker fragments of which only 44 (65.7%) revealed polymorphism.…”

Section: The Genomics Eramentioning

confidence: 99%

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Winged bean (Psophocarpus tetragonolobus (L.) DC.) for food and nutritional security: synthesis of past research and future direction

Tanzi

Eagleton

et al. 2019

Planta

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Main Conclusion Winged bean is popularly known as "One Species Supermarket" for its nutrient-dense green pods, immature seeds, tubers, leaves, and mature seeds. This underutilised crop has potential beneficial traits related to its biological nitrogen-fixation to support low-input farming. Drawing from past knowledge, and based on current technologies, we propose a roadmap for research and development of winged bean for sustainable food systems. Reliance on a handful of "major" crops has led to decreased diversity in crop species, agricultural systems and human diets. To reverse this trend, we need to encourage the greater use of minor, "orphan", underutilised species. These could contribute to an increase in crop diversity within agricultural systems, to improve human diets, and to support more sustainable and resilient food production systems. Among these underutilised species, winged bean (Psophocarpus tetragonolobus) has long been proposed as a crop for expanded use particularly in the humid tropics. It is an herbaceous perennial legume of equatorial environments and has been identified as a rich source of protein, with most parts of the plant being edible when appropriately prepared. However, to date, limited progress in structured improvement programmes has restricted the expansion of winged bean beyond its traditional confines. In this paper, we discuss the reasons for this and recommend approaches for better use of its genetic resources and related Psophocarpus species in developing improved varieties. We review studies on the growth, phenology, nodulation and nitrogen-fixation activity, breeding programmes, and molecular analyses. We then discuss prospects for the crop based on the greater understanding that these studies have provided and considering modern plant-breeding technologies and approaches. We propose a more targeted and structured research approach to fulfil the potential of winged bean to contribute to food security.

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Section: The Genomics Erasupporting

confidence: 83%

Section: Synthesis Of Past Researchmentioning

confidence: 99%

Section: The Genomics Eramentioning

confidence: 99%

See 1 more Smart Citation

Winged bean (Psophocarpus tetragonolobus (L.) DC.) for food and nutritional security: synthesis of past research and future direction

Tanzi

Eagleton

et al. 2019

Planta

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“…Despite its potential, winged bean has received limited research investment for developing molecular tools that can support breeding programmes, until recently. Recent reports include the development of inter-Simple Sequence Repeats (iSSRs) and Randomly Amplified Polymorphic DNA (RAPD) markers for genetic diversity and for clonal fidelity analyses and two small transcriptomic assemblies derived from a mix of leaf, bud, and shoot of Sri Lankan accessions and leaf tissue from a Nigerian accession, respectively [13,14,15,16,17]. Given that winged bean is believed to be largely self-pollinated, heterozygosity would be expected to be low, although a formal assessment is needed and the species does produce large flowers, suggesting a contribution from insect pollination, as recorded by Erskine [18].…”

Section: Introductionmentioning

confidence: 99%

Development of Gene‐Based SSR Markers in Winged Bean (Psophocarpus tetragonolobus (L.) DC.) for Diversity Assessment

Wong

Tanzi

et al. 2017

Genes

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Winged bean (Psophocarpus tetragonolobus) is an herbaceous multipurpose legume grown in hot and humid countries as a pulse, vegetable (leaves and pods), or root tuber crop depending on local consumption preferences. In addition to its different nutrient-rich edible parts which could contribute to food and nutritional security, it is an efficient nitrogen fixer as a component of sustainable agricultural systems. Generating genetic resources and improved lines would help to accelerate the breeding improvement of this crop, as the lack of improved cultivars adapted to specific environments has been one of the limitations preventing wider use. A transcriptomic de novo assembly was constructed from four tissues: leaf, root, pod, and reproductive tissues from Malaysian accessions, comprising of 198,554 contigs with a N50 of 1462 bp. Of these, 138,958 (70.0%) could be annotated. Among 9682 genic simple sequence repeat (SSR) motifs identified (excluding monomer repeats), trinucleotide-repeats were the most abundant (4855), followed by di-nucleotide (4500) repeats. A total of 18 SSR markers targeting di- and tri-nucleotide repeats have been validated as polymorphic markers based on an initial assessment of nine genotypes originated from five countries. A cluster analysis revealed provisional clusters among this limited, yet diverse selection of germplasm. The developed assembly and validated genic SSRs in this study provide a foundation for a better understanding of the plant breeding system for the genetic improvement of winged bean.

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“…However, the ISSR-PCR technique overcomes most of these disadvantages (ZIETKIEWICZ et al, 1994, MONDAL 2002, NYBOM, 2004, DAN, 2006, THOMAS et al, 2006, ANDERSON et al, 2010, KALPANA et al, 2012. The ISSR technique is a very simple and quick method to determine genetic diversity (ZIETKIEWICZ et al, 1994, MONDAL, 2002, DAN, 2006, CHEN et al, 2015. ISSR markers have been widely used to investigate the genetic relationships within and among populations of the genus Camellia in India, China, and Taiwan, as in other plants (MONDAL, 2002, DAN, 2006, THOMAS et al, 2006, CHEN et al, 2007, YAO et al, 2008, ROY and CHAKRABORTY, 2009, BEN-YING et al, 2010.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of genetic diversity of cultivated tea clones (Camellia sinensis (L.) Kuntze) in the eastern black sea coast by inter-simple sequence repeats (ISSRS)

et al. 2016

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Tea is the most globally consumed drink after spring water and an important breeding plant with high economical value in Turkey. In half a century, various kinds of tea cultivars have been bred in Turkey to improve the quality and yield of tea plants. Since tea reproduces sexually, tea fields vary in quality. Thus, determining the genetic diversity and relationship of the plants to support breeding and cultivation is important. In this study we aimed to determine the genetic diversity of tea cultivars breeding in the Eastern Black Sea coast of Turkey and the genetic relationship between them, to verify whether the qualitative morphological designations of the clones are genetically true by the ISSR markers. Herein, the genetic diversity and relationships of 18 Turkish tea cultivars were determined using 15 ISSR markers with sizes ranging from 250 to 3000 base pairs. The similarity indices among these cultivars were between 0.456 and 0.743. Based on cluster analysis using UPGMA, some of tea cultivars originating from the same geographical position were found to be clustered closely. Our data provide valuable information and a useful basis to assist selection and cloning experiments of tea cultivars and also help farmers to find elite parental clones for tea breeding in the Eastern Black Sea coast of Turkey.

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Genetic diversity evaluation of winged bean (Psophocarpus tetragonolobus (L.) DC.) using inter-simple sequence repeat (ISSR)

Cited by 21 publications

References 10 publications

Winged bean (Psophocarpus tetragonolobus (L.) DC.) for food and nutritional security: synthesis of past research and future direction

Winged bean (Psophocarpus tetragonolobus (L.) DC.) for food and nutritional security: synthesis of past research and future direction

Development of Gene‐Based SSR Markers in Winged Bean (Psophocarpus tetragonolobus (L.) DC.) for Diversity Assessment

Evaluation of genetic diversity of cultivated tea clones (Camellia sinensis (L.) Kuntze) in the eastern black sea coast by inter-simple sequence repeats (ISSRS)

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