Genetic Diversity and Variability in two Italian Autochthonous Donkey Genetic Types Assessed by Microsatellite Markers

Matassino, D.; Cecchi, Francesca; Ciani, F.; Incoronato, C.; Occidente, M.; Santoro, Laura; Ciampolini, Roberta

doi:10.4081/ijas.2014.3028

Cited by 14 publications

(24 citation statements)

References 30 publications

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“…Although in this study we used genetic profiles at the nuclear DNA level of only seven adult Banat donkey females, all the parameters of genetic diversity calculated for this group were similar or even higher compared to those observed in the two larger sub-populations of Balkan donkey females (Table 4), and also in populations of other donkey breeds distributed worldwide (Jordana, Folch & Sanchezm, 1999;Jordana et al, 2016;Aranguren-Mendez, Jordana & Gomez, 2001;Ivankovic et al, 2002;Blasi et al, 2005;Ciampolini et al, 2007;Zhu et al, 2013;Matassino et al, 2014;Rosenbom et al, 2015). Furthermore, two private alleles were observed in Banat donkeys characterized also by the significant excess of heterozygotes.…”

Section: Genetic Characterization Of the Banat Donkeymentioning

confidence: 64%

Banat donkey, a neglected donkey breed from the central Balkans (Serbia)

Stanišić

Aleksić

Dimitrijević

et al. 2020

PeerJ

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The dominant donkey breed in the Balkans is the mid-sized Balkan donkey with a grey to chocolate coat color. Local breeders from Serbia, however, still maintain a few larger individuals of a lighter coat color, named Banat donkey, and speculate that they are descendants of a Spanish donkey heard that had been transferred to the Banat region by the Hapsburg Queen Maria Theresa in the XVIII century for a specific purpose, to work in local vineyards. We have previously found a unique nuclear gene-pool and a prevalence of mitochondrial Clade 2 haplotypes in several such animals. In this study, we: (i) perform a comparative analysis of 18 morphological traits of the Banat donkey (seven individuals), Balkan donkey (53 individuals from two sub-populations of this breed) and the potential hybrids (eight individuals), and demonstrate the morphological distinctiveness of the Banat donkey, highlighting the diagnostic traits for distinguishing the breed: hip height, croup width, body length and chest depth; (ii) reanalyse published nuclear microsatellite data for these groups, and reveal that, although severely depopulated, the genetically distinct Banat donkey is not severely affected by the loss of genetic diversity and inbreeding; (iii) demonstrate that previously published Banat donkey mitochondrial haplotypes, analyzed genealogically together with those reported in ancient and modern individuals from Spain, Italy, Turkey, Cyprus and Africa, are shared with three Spanish breeds and individuals belonging to Amiata and some other Italian breeds. A unique morphological feature present in Banat and Somali wild donkeys, but also in Amiata donkeys, black stripes on legs, suggests that the origin of Clade 2 donkeys may be much more complex than previously thought. Actions to preserve the Banat donkey, a valuable but critically endangered genetic resource (<100 individuals), are urgent. How to cite this article Stanisic L, Aleksić JM, Dimitrijevic V, Kovačević B, Stevanovic J, Stanimirovic Z. 2020. Banat donkey, a neglected donkey breed from the central Balkans (Serbia). PeerJ 8:e8598 http://doi.

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Section: Genetic Characterization Of the Banat Donkeymentioning

confidence: 64%

Banat donkey, a neglected donkey breed from the central Balkans (Serbia)

Stanišić

Aleksić

Dimitrijević

et al. 2020

PeerJ

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“…As far as mean He of all loci be concerned, Chinese donkey breeds were similar to other highly diversified breeds in the Near East, northeast Africa (Rosenbom et al 2015), Spain (Aranguren-Mendez et al 2001), and Croatia (Ivankovic et al 2002), and more diversified than Italian (Bordonaro et al 2012;Colli et al 2013;Matassino et al 2014) and American (Jordana et al 2015) donkeys. For He of the same single locus in comparable studies, Chinese donkeys were also similar to or slightly higher than European (Aranguren-Mendez et al 2001;Jordana et al 2001;Ivankovic et al 2002;Bordonaro et al 2012;Colli et al 2013) and American (Jordana et al 2015) donkeys.…”

Section: Discussionmentioning

confidence: 82%

“…To date, the genetic diversity of indigenous donkey breeds in Spain (Aranguren-Mendez et al 2001;Aranguren-Mendez et al 2002), Croatia (Ivankovic et al 2002), Italy (Bordonaro et al 2012;Colli et al 2013;Matassino et al 2014), America (Jordana et al 2015), northeast Africa, the Near East and the Arabian Peninsula (Rosenbom et al 2015) has been evaluated using microsatellite markers, and these results of evaluation are crucial for making further conservation strategy. On the other hand, the origin of the species and evolutionary relationship between donkey breeds were analyzed using the mitochondrial DNA markers (Ivankovic et al 2002;Lopez Lopez et al 2005;Chen et al 2006;Zhang et al 2010;Han et al 2014;Perez-Pardal et al 2014), and these results hinted that the modern donkey (including Chinese donkey) has an African maternal origin.…”

mentioning

confidence: 99%

Evaluation of genetic diversity and population structure of five Chinese indigenous donkey breeds using microsatellite markers

Di¹,

Liu²,

F³

et al. 2017

Czech J. Anim. Sci.

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China had the largest population of raising donkeys in the world, however the number of Chinese indigenous donkey decreased dramatically due to the increase of agriculture mechanization in the last century. The species has still been important in China because of its edible and medical value, therefore the survey on its genetic diversity in China is necessary for its conservation and utilization. In this study, 15 microsatellite markers were used to evaluate genetic diversity and population structure of five Chinese indigenous donkey breeds. The mean values of expected heterozygosity, allelic richness, and total number of alleles for all the tested Chinese donkeys were 0.70, 6.04, and 6.28 respectively, suggesting that the genetic diversity of Chinese indigenous donkeys is rich. The Bayesian analysis and principal component analysis plot yielded the same clustering result, which revealed that Guanzhong donkey was the most differentiated breed in all detected samples, and Jinnan (JN) and Guangling (GL) were genetically closed together. Additionally, our results indicated that the heterozygote deficit was severe in two Chinese indigenous donkey breeds (GL and JN), and it warned us that animal conservation activities on this species should be considered carefully in near future.

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“…The Fstatistic values (F I S , fixation indices of subpopulation; F I T , fixation indices of total population; F ST , fixation index resulting from comparing subpopulations to the total population; Weir and Cockerham, 1984), together with the total number of alleles (At), were estimated with Arlequin version 3.1 (http://cmpg.unibe.ch/software/arlequin3, last access: 27 February 2019). The polymorphic information content (PIC) of each locus was calculated using PIC CALC (Nagy et al, 2012). The number of private alleles (NPA) was counted using the GDA program (https://download.csdn.net/ download/vip8_8/9856774, last access: 27 February 2019)…”

Section: Discussionmentioning

confidence: 99%

“…Autosomal microsatellite markers have been widely used in revealing genetic variability and identifying the genetic relationships among donkey populations (Jordana et al, 2001;Matassino et al, 2014;Rosenbom et al, 2015). Bordonaro et al (2012) described the genetic variability and differentiation in Pantesco and two other Sicilian autochthonous donkey breeds by microsatellites makers.…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity and relationships of Chinese donkeys using microsatellite markers

et al. 2019

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Donkeys are one important livestock in China because of their nourishment and medical values. To investigate the genetic diversity and phylogenetic relationships of Chinese donkey breeds, a panel of 25 fluorescently labeled microsatellite markers was applied to genotype 504 animals from 12 Chinese donkey breeds. A total of 226 alleles were detected, and the expected heterozygosity ranged from 0.6315 (Guanzhong) to 0.6999 (Jiami). The mean value of the polymorphism information content, observed number of alleles, and expected number of alleles for all the tested Chinese donkeys were 0.6600, 6.890, and 3.700, respectively, suggesting that Chinese indigenous donkeys have relatively abundant genetic diversity. Although there were abundant genetic variations found, the genetic differentiation between the Chinese donkey breeds was relatively low, which displayed only 5.99 % of the total genetic variance among different breeds. The principal coordinates analysis clearly splits 12 donkey breeds into two major groups. The first group included Xiji, Xinjiang, Liangzhou, Kulun, and Guanzhong donkey breeds. In the other group, Gunsha, Dezhou, Biyang, Taihang, Jiami, Qingyang, and Qinghai donkeys were clustered together. This grouping pattern was further supported by structure analysis and neighbor-joining tree analysis. Furthermore, genetic relationships between different donkey breeds identified in this study were corresponded to their geographic distribution and breeding history. Our results provide comprehensive and precise baseline information for further research on preservation and utilization of Chinese domestic donkeys.

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Genetic Diversity and Variability in two Italian Autochthonous Donkey Genetic Types Assessed by Microsatellite Markers

Cited by 14 publications

References 30 publications

Banat donkey, a neglected donkey breed from the central Balkans (Serbia)

Banat donkey, a neglected donkey breed from the central Balkans (Serbia)

Evaluation of genetic diversity and population structure of five Chinese indigenous donkey breeds using microsatellite markers

Genetic diversity and relationships of Chinese donkeys using microsatellite markers

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