Genetic diversity and symbiotic compatibility among rhizobial strains and Desmodium incanum and Lotus spp. plants

Abstract

This work aimed to evaluate the symbiotic compatibility and nodulation efficiency of rhizobia isolated from Desmodium incanum, Lotus corniculatus, L. subbiflorus, L. uliginosus and L. glaber plants by cross-inoculation. Twelve reference strains and 21 native isolates of rhizobia were genetically analyzed by the BOX-PCR technique, which showed a high genetic diversity among the rhizobia studied. The isolates were also characterized based on their production of indolic compounds and siderophores, as well as on their tolerance to salinity. Fifteen of the 33 rhizobia analyzed were able to produce indolic compounds, whereas 13 produced siderophores. All the tested rhizobia were sensitive to high salinity, although some were able to grow in solutions of up to 2% NaCl. Most of the native rhizobia isolated from L. uliginosus were able to induce nodulation in all plant species studied. In a greenhouse experiment using both D. incanum and L. corniculatus plants, the rhizobia isolate UFRGS Lu2 promoted the greatest plant growth. The results demonstrate that there are native rhizobia in the soils of southern Brazil that have low host specificity and are able to induce nodulation and form active nodules in several plant species.

Biodiversity of Rhizobia That Nodulate Melilotus indicus L. in Egyptian Soils

“…It allows the fingerprinting of individual genera, species, and strains and could help to determine phylogenetic relationships. Also, these findings are in agreement with the results obtained by other authors who studied diversity among natural rhizobial populations in different world regions (Adiguzel et al, 2009 andGranada et al, 2014).…”

Diversity of new root nodule bacteria from Erythrina velutina Willd., a native legume from the Caatinga dry forest (Northeastern Brazil)

“…Among the cultivated species, studies conducted in Brazil have already examined the genetic variability of rhizobia of legumes such as pigeonpea (Costa et al, 2014), peanuts (Lyra et al, 2013;TorresJúnior et al, 2014), common beans (Stocco et al, 2008), among others. This technique is also used to examine the variability of strains isolated from native plants such as bromeliad (Giongo et al, 2013), wild rice (Fernandes Júnior et al, 2013) and legumes (Granada et al, 2014). The results obtained in the present study, demonstrate its applicability and indicate that this technique can be used with rhizobia of other native tree legumes.…”

“…Phenotypic characterization is the basis of polyphasic taxonomy and, together with molecular techniques, has contributed to an improved understanding of the physiology and genetic diversity of rhizobia (Zerhari et al, 2000;Fall et al, 2008;Rasul et al, 2012). Among the molecular characterization techniques, fingerprinting by means of Box-PCR generated profiles have been used to examine the diversity of diazotrophic bacteria associated with the (Stocco et al, 2008;Costa et al, 2014;Torres-Júnior et al, 2014) and native species (Fernandes Júnior et al, 2013;Granada et al, 2014) from Brazil. This simple and low-cost technique allows the discrimination among several rhizobial isolates and reference strains, making possible a fast fingerprinting and selection of bacterial isolates.…”

Interações Bióticas Entre Actinobactérias E Rizóbios Em Solos Da Região Semiárida Brasileira

“…Durante o processo simbiótico, os rizóbios reduzem o nitrogênio atmosférico em uma forma diretamente assimilável pela planta (GRANADA et al, 2014;PINHEIRO et al, 2014;MUS et al, 2016).…”

“…O rizóbio simbiótico em plantas não-nativas pode induzir a adaptação das plantas através de efeitos indiretos, incluindo diminuição da severidade de doenças e melhoria nas interações bacterianas mútuas e efeitos diretos, incluindo alterações nos processos ecológicos e ecossistêmicos, produção de hormônios e solubilização de nutrientes do solo (GRANADA et al, 2014;DEAN et al, 2014).…”