Genetic diversity and structure of native and non-native populations of the endangered plant Pinus dabeshanensis

Abstract

ABSTRACT. Owing to a severe decline in its abundance, Pinus dabeshanensis has been listed as an endangered species by the International Union for the Conservation of Nature. Although several restoration events have been undertaken since the 1960s, the natural population genetic structure of this species remains to be investigated. Herein, we examined the level of genetic diversity and structure of two native and two non-native populations using 10 microsatellite loci. A relatively high level of genetic variation (H O = 0.586 ± 0.039) and a low level of population differentiation (F ST = 0.016 ± 0.011) were revealed. For forensic investigation, an assignment test was performed. To better understand the genetic differentiation between the native and nonnative populations, the individuals in the transplanted and cultivated populations may have derived from populations that were not surveyed 2 Z.Y. Zhang et al. ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 15 (2): gmr.15027937 in this study. In light of our results, we discuss the real problems faced by all four populations and provide useful information for management decision-making.

Population Genetic Analysis of Pinus Koraiensis in China Inferred Using EST-SSR Markers

“…In the present study, the PIC values obtained for the multiallelic EST-SSR markers ranged from 0.142 to 0.833, with a mean value of 0.461, indicating high level of genetic information among the 480 P. koraiensis individuals from the 16 natural populations. The genetic diversity of P. koraiensis obtained in the present study is higher than that reported in Pinus bungeana (Na = 3.70, He = 0.36) [39], Pinus dabeshanensis (He = 0.36) [40] and Pinus yunnanensis (Na = 4.10, He = 0.43) [41] but lower than that reported in Pinus tabulaeformis (Na = 6.52, He = 0.68) [42].…”

EST‐SSR‐based landscape genetics ofPseudotaxus chienii, a tertiary relict conifer endemic to China

“…likiangensis ( He = 0.7186) (Cheng et al., 2014); close to P . dabeshanensis ( He = 0.36) (Zhang et al., 2016) and Amentotaxus argotaenia ( He = 0.39) (Ruan et al, 2019); and higher than A . formosana ( He = 0.1993) (Li et al., 2016), P .…”

“…Pinus massoniana (He = 0.5717) (Zhang et al, 2014), Picea abies (He = 0.616) (Stojnić et al, 2019), and P. likiangensis (He = 0.7186) (Cheng et al, 2014); close to P. dabeshanensis (He = 0.36) (Zhang et al, 2016) and Amentotaxus argotaenia (He = 0.39) (Ruan et al, 2019); and higher than A. formosana (He = 0.1993) (Li et al, 2016), P. bungeana (He = 0.205) (Duan et al, 2017), and A. yunnanensis (He = 0.3343) (Li et al, 2016 history, natural selection, and mutation rate (Hamrick et al, 1992;Su et al, 2009;Wang et al, 2020).…”

Genetic Evaluation of Natural Populations of the Endangered Conifer Thuja koraiensis Using Microsatellite Markers by Restriction-Associated DNA Sequencing

“…Compared with relatively abundant species, especially their widespread congeners, rare species, endemic plants and endangered species usually exhibit lower genetic diversity [ 71 , 72 ]. However, the values determined here represented higher SSR-derived genetic diversity than in other endangered endemic perennial species, such as Paeonia jishanensis (mean N A = 2.376; H E = 0.340 within 236 individuals from 10 extant populations) [ 73 ], Taxus wallichiana (mean N A = 4.154; H E = 0.538 within 130 individuals from 13 geographically separate populations) [ 74 ], and Pinus dabeshanensis (mean N A = 3.700; H E = 0.360 within 148 samples from four extant populations) [ 75 ]. The average value of H O (0.644) was slightly greater than that of H E (0.548), and the value of F was negative at the population level, indicating that excess heterozygosity was existed within the entire natural distribution range of the species.…”