Genetic diversity and structure of Astrocaryum jauari (Mart.) palm in two Amazon river basins

Oliveira, Liliane dos Santos; Ramos, Santiago Linório Ferreyra; Lopes, Maria Teresa Gomes; Dequigiovanni, Gabriel; Veasey, Elizabeth Ann; Macêdo, J. L. V. de; Batista, Jacqueline S.; Formiga, Kyara Martins; Lopes, Ricardo

doi:10.1590/1984-70332014v14n3a25

Cited by 10 publications

(13 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Nevertheless, similar results for H E and H O were found for Chamaerops humilis, with a mean N A equal to 3.67 and H E and H O equal to 0.374 and 0.366, respectively (Giovino et al, 2014). It is worth mentioning that studies with other congeners of Astrocaryum (Oliveira et al, 2014;Ramos et al, 2012Ramos et al, , 2016) that used the same microsatellite set used here, excluded monomorphic loci from the estimation of genetic diversity; this automatically increased the estimates for these parameters [given the formula used to calculate heterozygosity (H = 2 ( 1)…”

Section: Discussionsupporting

confidence: 72%

“…Other population genetic structure parameters, such as Wright's inbreeding coefficient and gene flow, have shown that, in general, genetic differentiation (F ST ) is low, indicating genetic variation higher within -rather than among -populations and a high gene flow among populations (Elshibli and Korpelainen, 2008;Martins-Corder et al, 2009;Shapcott et al, 2009;Namoff et al, 2011;Silva et al, 2011;Giovino et al, 2014;Nazareno and dos Reis, 2014;Oliveira et al, 2014;Moura et al, 2015). Recently, Ramos et al (2016) found a high level of genetic diversity, mostly within populations, for adults and seedlings in populations of A. aculeatum, and along with results for spatial genetic structure indicated short-distance seed dispersal and modest levels of pollen flow.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Research Article Population genetic structure of three species in the genus Astrocaryum G. Mey. (Arecaceae).

Oliveira

Davide

et al. 2017

Genet. Mol. Res.

View full text Add to dashboard Cite

ABSTRACT.We assessed the level and distribution of genetic diversity in three species of the economically important palm genus Astrocaryum located in Pará State, in northern Brazil. Samples were collected in three municipalities for Astrocaryum aculeatum: Belterra, Santarém, and Terra Santa; and in two municipalities for both A. murumuru: Belém and Santo Antônio do Tauá and A. paramaca: Belém and Ananindeua. Eight microsatellite loci amplified well and were used for genetic analysis. The mean number of alleles per locus for A. aculeatum, A. murumuru, and A. paramaca were 2.33, 2.38, and 2.06, respectively. Genetic diversity was similar for the three species, ranging from H E = 0.222 in A. aculeatum to H E = 0.254 in A. murumuru. Both F ST and AMOVA showed that most of the genetic variation was found within populations for all three species, but high genetic differentiation among populations was found for A. aculeatum. Three loci were not in HardyWeinberg equilibrium, with populations of A. paramaca showing a tendency for the excess of heterozygotes (F IS = -0.144). Gene flow was 2 N.P. Oliveira et al. Genetics and Molecular Research 16 (3): gmr16039676 high for populations of A. paramaca (N m = 19.35). Our results suggest that the genetic diversity within populations followed the genetic differentiation among populations due to high gene flow among the population. Greater geographic distances among the three collection sites for A. aculeatum likely hampered gene flow for this species.

show abstract

Section: Discussionsupporting

confidence: 72%

Section: Introductionmentioning

confidence: 99%

Research Article Population genetic structure of three species in the genus Astrocaryum G. Mey. (Arecaceae).

Oliveira

Davide

et al. 2017

Genet. Mol. Res.

View full text Add to dashboard Cite

show abstract

“…In palms, Oliveira et al (2014) found that the genetic structure of Astrocaryum jauari among different river sites within a river basin with high levels of gene flow within them, likely due to transport of fruits following the direction of the water currents. Mauritia swamps are a permanent or temporary shelter for many species of animals that maintain gene flow via seed dispersal (Mendieta-Aguilar, Pacheco, & Roldán, 2015), and fruits have the ability to undergo long-distance dispersal and float down rivers, traveling thousands of kilometers, connecting populations at large distances along the water (Moegenburg, 2002).…”

Section: Rivers Are Determinants Of Mauritia Flexuosa Population Stmentioning

confidence: 99%

Rivers shape population genetic structure in Mauritia flexuosa (Arecaceae)

Sander

Pérez-Zavala

Silva

et al. 2018

Ecology and Evolution

View full text Add to dashboard Cite

The Mauritia flexuosa L.f. palm is known as the “tree of life” given its importance as fundamental food and construction resources for humans. The species is broadly distributed in wet habitats of Amazonia and dry habitats of the Amazon and Orinoco river basins and in the Cerrado savanna. We collected 179 individuals from eight different localities throughout these habitats and used microsatellites to characterize their population structure and patterns of gene flow. Overall, we found high genetic variation, except in one savanna locality. Gene flow between populations is largely congruent with river basins and the direction of water flow within and among them, suggesting their importance for seed dispersal. Further, rivers have had a higher frequency of human settlements than forested sites, contributing to population diversity and structure through increased human use and consumption of M. flexuosa along rivers. Gene flow patterns revealed that migrants are sourced primarily from within the same river basin, such as those from Madeira and Tapajós basins. Our work suggests that rivers and their inhabitants are a critical element of the landscape in Amazonia and have impacted the dispersal and subsequent distribution of tropical palm species, as shown by the patterns of genetic variation in M. flexuosa.

show abstract

“…In the present study, storage of leaflets in transport buffer for one week also proved to be a viable methodology for the preservation of plant tissue, since these leaflets retained the characteristics of fresh leaflets. Despite producing colour alteration in plant tissue, the silica gel method has been commonly used in the preservation of leaf samples of palm species such as "tucumanzeiro" (A. aculeatum), "açaizeiro" (E. oleracea), and "jauari" (A. jauari) (MILANI et al, 2009;OLIVEIRA et al, 2010;OLIVEIRA et al, 2014). The silica gel storage method has several advantages over other methods, owing to its simplicity, low cost, and the minimal space required for transport and storage in the laboratory.…”

Section: Resultsmentioning

confidence: 99%

Preservation and maceration of amazon açai leaflet tissue to obtain genomic DNA

et al. 2019

View full text Add to dashboard Cite

Biomedicina -Faculdade Meta -FAMETA, Rio Branco, AC, Brasil.; 3. Professor, Doutor, Instituto Federal do Acre -IFAC, Cruzeiro do Sul, AC, Brasil.; 4. Analista da Empresa Brasileira de Pesquisa Agropecuária -Embrapa Acre, Rio Branco, AC, Brazil.; 5. Pesquisadora, Doutora, Empresa Brasileira de Pesquisa Agropecuária -Embrapa Rondônia, Porto Velho, RO, Brasil.; 6. Pesquisadora, Doutora, Empresa Brasileira de Pesquisa Agropecuária -Embrapa Acre, Rio Branco, AC, Brasil, tatiana.campos@embrapa.br. ABSTRACT:The objective of this study was to test the efficiency of preservation and maceration methods for Euterpe precatoria leaflet tissue to obtain genomic DNA for molecular studies. The leaflets of E. precatoria were collected in an experimental field at Embrapa Acre, Brazil. The study was conducted in a completely randomized design with 10 replicates, in a 12 × 2 factorial structure, with 12 storage treatments (fresh; lyophiliser 3 days; refrigerator 3, 5, and 7 days; silica gel 7, 10, 20, and 30 days; and transport buffer 3, 5, and 7 days) and two leaf tissue maceration methods (liquid nitrogen and the TissueLyser®). Statistically significant differences in the obtained DNA concentration were found between the maceration and storage treatments. The TissueLyser® macerator produced higher DNA concentrations when compared to liquid nitrogen. For the storage treatments, five groups were formed based on DNA concentration when macerated with the TissueLyser® and two groups when macerated with liquid nitrogen. The DNA concentrations ranged from 285.00 ng/µL (7 days in transport buffer) to 702.00 ng/µL (30 days in silica gel) when the leaflets were macerated with liquid nitrogen, and from 572.73 ng/µL (30 days in silica gel) to 2,850.00 ng/µL (3 days in lyophiliser) using the TissueLyser® macerator. The DNA purity (A260/A280 nm) varied from 1.30 to 1.70 when the leaflets were macerated with liquid nitrogen and from 1.30 to 1.90 with the TissueLyser® macerator. Despite the variations in leaf tissue preservation and DNA concentration, all treatments were effective for DNA isolation and it was possible to amplify genomic regions of microsatellite markers by PCR. It was concluded that leaflets of E. precatoria stored in a lyophiliser and processed with an automatic macerator resulted in satisfactory DNA for molecular studies.

show abstract

Genetic diversity and structure of Astrocaryum jauari (Mart.) palm in two Amazon river basins

Abstract: -Astrocaryum jauari is a non-domesticated palm that is

Cited by 10 publications

References 35 publications

Research Article Population genetic structure of three species in the genus Astrocaryum G. Mey. (Arecaceae).

Research Article Population genetic structure of three species in the genus Astrocaryum G. Mey. (Arecaceae).

Rivers shape population genetic structure in Mauritia flexuosa (Arecaceae)

Preservation and maceration of amazon açai leaflet tissue to obtain genomic DNA

Contact Info

Product

Resources

About