Genetic diversity and structure of Munronia delavayi Franch. (Meliaceae), an endemic species in the dry-hot valley of Jinsha River, south-western China

Jia, Jing; Wu, Hao; Wang, Jinfeng; Gong, Xun

doi:10.1007/s10722-014-0120-7

Cited by 7 publications

(7 citation statements)

References 40 publications

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“…Unlike those reported inland Cycas species (Liu et al 2015;Zheng et al 2016;Yang et al 2017) and the sympatric species distributed in the dry and hot valley of Jinsha River: Munronia delavayi (Jia et al 2014), and Nouelia insignis (Gong et al 2011), no significant spatial genetic structure of C. panzhihuaensis was revealed by previous research (Wang et al 2010;Zhang 2012;Yang et al 2015) and our study. Most private haplotypes are found in WQ, SL, GH, which are small populations mostly located in the boundary of the distribution range.…”

Section: Demographical Historycontrasting

confidence: 99%

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Supplemental Information 12: Figure Plot of Geographical Distance (X Axial) Against Genetic Distance (F_ST, Y

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Background Cycas panzhihuaensis L. Zhou & S. Y. Yang (Cycadaceae) is an endangered gymnosperm species endemic in the dry-hot valley of Jinsha River basin from southwest China. Although the wild C. panzhihuaensis population from Panzhihua Cycad Natural Reserve is well protected, other known populations that fall outside the natural reserve may preserve specific genetic resources while face with larger extinction risk because of lacking essential monitoring. Methods In this study, we analyzed the genetic diversity, phylogeographical structure and demographic history of C. panzhihuaensis from seven known locations so far by sequencing three chloroplastic DNA regions (psbA-trnH, psbM-trnD, and trnS-trnG), four single-copy nuclear genes (PHYP, AC5, HSP70, and AAT) from 61 individuals, and eleven microsatellite loci (SSR) from 102 individuals. Results and Discussion We found relative high genetic diversity within populations and high genetic differentiation among the populations of C. panzhihuaensis, which is similar with the other Asian inland cycads. Despite no significant phylogeographical structure was detected, small and unprotected populations possess higher genetic diversity and more unique haplotypes, which deserve due attention. Results of demographic dynamics suggest that human activity is the key factor that leads C. panzhihuaensis to endangered status. Basing on the genetic characterization of C. panzhihuaensis, we proposed several practical guidelines for the conservation of this species, especially for its small populations.

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Section: Demographical Historycontrasting

confidence: 99%

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Supplemental Information 12: Figure Plot of Geographical Distance (X Axial) Against Genetic Distance (F_ST, Y

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“…The genetic structure of existing populations can be imprinted by historical processes (e.g., ice age), especially for those long-evolved and sessile organisms (Feng et al, 2014 ). Genetic data can provide insights into adaptive potential for particular species in postglacial colonization refugia as well as valuable information and suggestions for the species delimitation, demographic history and conservation categories (Gong et al, 2011 ; Zhao and Gong, 2012 ; Jia et al, 2014 ). In this study, we sequenced three maternally inherited cpDNA and two biparentally inherited nDNA markers of 15 populations from C. diannanensis, C. parvula , and C. multiovula , which shared an overlapped distribution in the Red River basin, and examined the genetic relationships between them.…”

Section: Introductionmentioning

confidence: 99%

Species delimitation, genetic diversity and population historical dynamics of Cycas diannanensis (Cycadaceae) occurring sympatrically in the Red River region of China

2015

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Delimitating species boundaries could be of critical importance when evaluating the species' evolving process and providing guidelines for conservation genetics. Here, species delimitation was carried out on three endemic and endangered Cycas species with resembling morphology and overlapped distribution range along the Red River (Yuanjiang) in China: Cycas diananensis Z. T. Guan et G. D. Tao, Cycas parvula S. L. Yang and Cycas multiovula D. Y. Wang. A total of 137 individuals from 15 populations were genotyped by using three chloroplastic (psbA-trnH, atpI-atpH, and trnL-rps4) and two single copy nuclear (RPB1 and SmHP) DNA sequences. Basing on the carefully morphological comparison and cladistic haplotype aggregation (CHA) analysis, we propose all the populations as one species, with the rest two incorporated into C. diannanensis. Genetic diversity and structure analysis of the conflated C. diannanensis revealed this species possessed a relative lower genetic diversity than estimates of other Cycas species. The higher genetic diversity among populations and relative lower genetic diversity within populations, as well as obvious genetic differentiation among populations inferred from chloroplastic DNA (cpDNA) suggested a recent genetic loss within this protected species. Additionally, a clear genetic structure of C. diannanensis corresponding with geography was detected based on cpDNA, dividing its population ranges into “Yuanjiang-Nanhun” basin and “Ejia-Jiepai” basin groups. Demographical history analyses based on combined cpDNA and one nuclear DNA (nDNA) SmHP both showed the population size of C. diannanensis began to decrease in Quaternary glaciation with no subsequent expansion, while another nDNA RPB1 revealed a more recent sudden expansion after long-term population size contraction, suggesting its probable bottleneck events in history. Our findings offer grounded views for clarifying species boundaries of C. diannanensis when determining the conservation objectives. For operational guidelines, the downstream populations which occupy high and peculiar haplotypes should be given prior in-situ conservation. In addition, ex-situ conservation and reintroduction measures for decades of generations are supplemented for improving the population size and genetic diversity of the endemic and endangered species.

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“…2), which include (1) A. glaucifolium (Guan et al 2013), M. delavayi (Jia et al 2014), A. delavayi (Yang et al 2014) and T. gracilis (unpublished) becoming endangered due to the hydropower stations construction, (2) N. insignis (Gong et al 2011;Luan et al 2006), Leucomeris decora (Zhao and Gong 2012) and Cycas hongheensis (only two populations left) (unpublished) with decreasing populations because of human exploitation for woods or habitat destruction, (3) Psammosilene tunicoides facing overexploitation threats for herb medicines (Zhang et al 2011b), and Citrus hongheensis which is also an important wild germplasm resource (Table 1). We attempt to provide insights into genetic diversity and guidance on conservation managements for these species.…”

Section: Conservation Of Plant Species In the Valleysmentioning

confidence: 99%

Diversity and conservation of plant species in dry valleys, southwest China

Zhao

Gong

2015

Biodivers Conserv

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The dry valleys in southwest China harbor a highly varied concentration of endemic plant species. To date, the conservation of the unique ecosystem has been paid attention, whereas conservation for the regional endemic plant species has not been much focused. The dry valleys own approximately 1707 plant species among which, 37.42 % are endemic. The plant species in that dry ecosystem are currently challenged by the climate change and anthropogenic activity. To give insight into the current (and past) status of threatened species and the population conservation management strategies, we investigated the genetic diversities of endemic and endangered plant species in the dry valleys in the last decade, using nuclear or plastid molecular markers. Our data revealed that the plant species in this region have low levels of genetic diversity within populations and strong genetic structure. We thus concluded that strong geography patterns are mainly shaped by complex topography as well as their local habitats. We pointed that it is of importance to incorporate neutral and non-neutral genetic diversity when assessing the status of the endangered species. We suggest more research findings are needed to well understand and ensure regional effective conservation on the plant species occurred in the dry valleys.

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Genetic diversity and structure of Munronia delavayi Franch. (Meliaceae), an endemic species in the dry-hot valley of Jinsha River, south-western China

Cited by 7 publications

References 40 publications

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Species delimitation, genetic diversity and population historical dynamics of Cycas diannanensis (Cycadaceae) occurring sympatrically in the Red River region of China

Diversity and conservation of plant species in dry valleys, southwest China

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