Genetic Diversity and Structure Analysis of a Worldwide Collection of Faba bean (Vicia faba) Genotypes using ISSR Markers

Abstract

The success of breeding programs depends on the extent of genetic variability. Inter-simple sequence repeats (ISSR) have been widely utilized in investigations, including the characterization of many plant species genetically. This research aimed to examine both the genetic diversity and relationships of 92 faba bean (Vicia faba L.) genotypes from different geographical areas using ISSR markers. Eleven ISSR primers generated a total of 189 repeatable amplified bands, of which 109 were polymorphic. Values of polymorphism information content (PIC) and gene diversity averaged 0.3484 and 0.1438 and ranged 0.089–0.715 and 0.0742–0.2065, respectively. The studied accessions of faba bean plant differentiated into four main clusters, prevalently based on geographical origin through UPGMA clustering analysis and principal component analysis (PCA), deriving four major groupings based on pedigree and origin relationships. The STRUCTURE software analysis results were significantly aligned with the PCA and showed five main clusters; each one represents one continent. AMOVA showed high variation and differentiation among nations from different continents. The discrimination power of ISSR markers obtained in this study suggests that they could be used to examine the diversity of faba bean genotypes efficiently and precisely and encourage targeted crossing strategies. © 2021 Friends Science Publishers

Agro-Morphological and Molecular Variability among Algerian Faba Bean (Vicia faba L.) Accessions

“…Moreover, as reported by Qahthn et al [40], African germplasm was characterized by the highest genetic diversity among a number of compared geographical locations. Considering that genetic diversity diminishes continuously [54], the new polymorphisms detected in our collection could provide a new genetic resource for improving faba beans [55].…”

“…Compared to other legume crops, faba bean production has markedly decreased due to different biotic and abiotic stresses [39], and the generation of appropriate varieties based on genetic diversity studies in order to overcome these stresses represents the main target, among a variety of strategies [40].…”

Assessing Drought Tolerance of some Faba Bean (Vicia faba L.) Cultivars Using Genetic Variability, Tolerance Indices and ISSR Markers

“…Drought tolerance was polygenic and complex trait effected with the environment causing difficulty in phenotypic evaluation while; the using of DNA markers could be help breeders in improving the speed and reliability of the evaluation process (Khan et al, 2013). ISSR technique was much suitable and precisely for resolving genetic diversity of closely related faba bean genotypes and for cultivar identification through high percent of polymorphism was obtained by ISSR primers (El-Nahas et al, 2011;Kianoosh et al, 2017, Asfaw et al, 2018Qahtan et al, 2021). specific markers; negative and positive molecular size (pb) for different ISSR primers used as marker assisted selection (MAS) and appear that ISSR markers are effective in faba bean breeding programs and encourage targeted crossing strategies for drought tolerance and genetic relationships between different faba bean cultivars (Belal et al, 2009;Afiah et al, 2016;Qahtan et al, 2021).…”