Genetic diversity and safety aspects of enterococci from slightly fermented sausages

Martín, Belén; Garriga, Margarita; Hugas, Marta; Aymerich, Teresa

doi:10.1111/j.1365-2672.2005.02555.x

Cited by 88 publications

(54 citation statements)

References 67 publications

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“…This prevalence of putative virulence factors is common among food-animal enterococci [46,49]. The virulence data for E. faecalis are also consistent with the hypothesis that the flies acquired E. faecalis from sludge.…”

Section: Discussionsupporting

confidence: 74%

Role of House Flies in the Ecology of Enterococcus faecalis from Wastewater Treatment Facilities

2013

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Enterococci are important nosocomial pathogens with Enterococcus faecalis most commonly responsible for human infections. In this study, we used several measures to test the hypothesis that house flies, Musca domestica (L.), acquire and disseminate antibiotic resistant and potentially virulent E.faecalis from wastewater treatment facilities (WWTF) to the surrounding urban environment. House flies and sludge from four WWTF (1-4) as well as house flies from three urban sites close to WWTF-1 were collected and cultured for enterococci. Enterococci were identified, quantified, screened for antibiotic resistance and virulence traits, and assessed for clonality. Of the eleven antibiotics tested, E. faecalis was most commonly resistant to tetracycline, doxycycline, streptomycin, gentamicin and erythromycin and these traits were intra-species horizontally transferrable by in vitro conjugation. Profiles of E. faecalis (prevalence, antibiotic resistance, and virulence traits) from each of WWTF sludge and associated house flies were similar, indicating that flies successfully acquired these bacteria from this substrate. The greatest number of E. faecalis with antibiotic resistance and virulence factors (i.e., gelatinase, cytolysin, enterococcus surface protein, and aggregation substance) originated from WWTF-1 that processed meat waste from a nearby commercial meat processing plant, suggesting an agricultural rather than human clinical source of these isolates. E. faecalis from house flies collected from three sites 0.7 -1.5 km away from WWTF-1 were also similar in their antibiotic resistance profiles; however, antibiotic resistance was significantly less frequent. Clonal diversity assessment using pulsed-field gel electrophoresis revealed the same clones of E. faecalis from sludge and house flies from WWTF-1 but not from the three urban sites close to WWTF-1. This study demonstrates that house flies acquire antibiotic resistant enterococci from WWTF and potentially disseminate them to the surrounding environment.

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Section: Discussionsupporting

confidence: 74%

Role of House Flies in the Ecology of Enterococcus faecalis from Wastewater Treatment Facilities

2013

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“…These results are similar to previous reports of Abriouel et al (1) regarding the absence of the cylA gene among vegetable food isolates. High rate of asa1 and cylA genes among our E. durans isolates is a worrying output as there are some reports suggesting that the presence of virulence traits in species other than E. faecalis and E. faecium is important for proper evaluation of virulence evolution within the Enterococcus genus (16,23). No significant difference was observed between cylA gene positivity and β-hemolytic activity in our study.…”

Section: Discussioncontrasting

confidence: 47%

The relationship between virulence factors and vancomycin resistance among Enterococci collected from food and human samples in Southern Turkey

ERGİNKAYA¹

2014

Ankara Üniversitesi Veteriner Fakültesi Dergisi

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Summary:The aims of this research were to study the prevalence of potential virulence factors, vancomycin resistance and also to evaluate a possible correlation that can exist between vancomycin resistance and potential virulence factors between 51 Enterococcus spp. isolated from food and 50 Enterococcus faecium strains from human in southern Turkey. Identification of the isolates was determined by Vitek-II system. Antimicrobial susceptibility tests were performed by Vitek-II system and disc diffusion method. The presence of vanA and vanB as well as enterococcal virulence genes of cytolysin (cylA), the aggregation substance (asa1), gelatinase (gelE), enterococal surface protein (esp), hyaluronidase (hyl) were investigated by Polymerase Chain Reaction (PCR) method. Haemolysin production was also studied phenotypic method. Apart from one isolate, none of the food originated enterococci were resistant to vancomycin, and none carried vanA and vanB resistance genes. All clinical isolates were resistant to vancomycin and 84% of them carried vanA; 2%, vanB; and 14%, neither vanA nor vanB genes. Except for the cylA gene, all other virulence genes and vancomycin resistance were higher in human strains, and a positive correlation was observed between multivirulence genes and hemolytic activity. For all strains, a positive correlation existed between the esp gene positivity and vancomycin resistance, while for only E. faecium, esp, hyl gene positivity and vancomycin resistance a positive correlation could be seen. Furthermore, "silent cylA" genes were found in two food and one intestinal strains. Based on our findings, we can suggest that virulence increases in parallel to vancomycin resistance, and food may be a potential source for dissemination of gelE, asa1 and hyl virulence genes. Finally, esp and hyl genes presence should carefully be monitored in food originated enterococci.Key words: Enterococci, relationship, vancomycin resistance, virulence factors. Türkiye'nin güneyinde gıda ve insan örneklerinden toplanan Enterokok izolatlarının vankomisin direnci ve virülans faktörleri arasındaki ilişki

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“…Finally, other researchers revealed that Enterococcus spp. strains from different sources could have the ability to reach the intestinal lumen and stay alive in that environment [28,47] However, further in vitro studies are needed with these strains in order to establish their probiotic potential. …”

Section: Screening For Probiotic Potentialmentioning

confidence: 99%

Enterococci Isolated from Cypriot Green Table Olives as a New Source of Technological and Probiotic Properties

2018

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Table olive is one of the main fermented vegetable worldwide and can be processed as treated or natural product. Lactic Acid Bacteria (LAB) are responsible for the fermentation of treated olives. The aim of this work was to study the technological characteristics and the potential probiotic properties of LAB isolated from Cypriot green table olives. This is the first comprehensive report on the isolation and characterization of LAB isolates retrieved from Cypriot green table olives. From a collection of 92 isolates from spontaneously fermenting green olives, 64 g positive isolates were firstly identified to genus level using biochemical tests, and secondly to species level using multiplex species specific polymerase chain reaction (PCR) amplifications of the sodA gene. Moreover, each of our isolates were tested for their technological and probiotics properties, as well as for their safety characteristics, using biochemical and molecular methods, in order to be used as starter cultures. Finally, to discriminate the most promising isolates on the base of their technological and probiotics properties, Principal component analysis was used. All the isolates were identified as Enteroccocus faecium, having interesting technological properties, while pathogenicity determinants were absent. Principal component analysis showed that some isolates had a combination of the tested parameters. These findings demonstrate that enteroccoci from Cypriot table olives should be considered as a new source of potential starter cultures for fermented products, having possibly promising technological and probiotic attributes.

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Genetic diversity and safety aspects of enterococci from slightly fermented sausages

Cited by 88 publications

References 67 publications

Role of House Flies in the Ecology of Enterococcus faecalis from Wastewater Treatment Facilities

Role of House Flies in the Ecology of Enterococcus faecalis from Wastewater Treatment Facilities

The relationship between virulence factors and vancomycin resistance among Enterococci collected from food and human samples in Southern Turkey

Enterococci Isolated from Cypriot Green Table Olives as a New Source of Technological and Probiotic Properties

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