Genetic diversity and recombination within bufaviruses: Detection of a novel strain in Hungarian bats

Kemenesi, Gábor; Dallos, Bianka; Görföl, Tamás; Estók, Péter; Boldogh, Sándor; Kurucz, Kornélia; Oldal, Miklós; Marton, Szilvia; Bányai, Krisztiàn; Jakab, Ferenc

doi:10.1016/j.meegid.2015.05.017

Cited by 37 publications

(34 citation statements)

References 17 publications

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confidence: 62%

“…The role of bufaviruses as an etiologic agent of human gastroenteritis has not been firmly established yet. Further metagenomic investigations show distinct lineages of bufavirus in wild shrews and non-human primates from Zambia (Sasaki et al, 2015), in Hungarian bats (Kemenesi et al, 2015) and pigs (Hargitai et al, 2016) and in Chinese rats (Yang et al, 2016). This report describes the identification and characterization of bufavirus in faeces of domestic pigs by a metagenomic approach.…”

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confidence: 88%

“…Bufaviruses found in bats, a rat and a shrew were distantly related to the porcine and human bufaviruses. Although the WUHARV parvovirus has been suggested to be a recombinant between bat and human bufaviruses (Kemenesi et al, 2015), this recombination signal was not detected by our conserved analysis, where a recombination signal should be detected by more than four out of all eight methods implemented in the program and at a highest acceptable P value of 0.001.Except for the single strain of human bufavirus 2 (accession number KM580349), the genetic diversities of human bufaviruses within each genotype and of porcine bufavirus are seemingly limited at the moment. This observation indicated that bufaviruses likely entered humans and domestic pigs a relatively short time ago and evolved independently in their respective hosts.…”

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confidence: 99%

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Identification of a novel bufavirus in domestic pigs by a viral metagenomic approach

Schwarz

Ullman

Ahola

et al. 2016

Journal of General Virology

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Bufavirus is a single-stranded DNA virus belonging to the genus Protoparvovirus. This study reports the identification and characterization of a porcine bufavirus by a metagenomic approach, and a limited epidemiology investigation of bufavirus in six swine farms. A comparative genome analysis showed a similarity of 93 % to a Hungarian porcine bufavirus. Bayesian and maximumlikelihood analyses of genome sequences showed a close relationship of porcine bufaviruses to human and monkey bufaviruses. Molecular dating of the most recent common ancestors supported a recent introduction of bufaviruses into human and pig populations, respectively. A real-time PCR method was developed to screen 60 faecal samples for the porcine bufavirus DNA, and eight positive samples were found in two neighbouring farms, suggesting a relatively low prevalence (13.3 %). No direct transmission of porcine bufaviruses between two neighbouring farms was found, suggesting that bufaviruses may have spread widely in different geographical regions.Bufavirus belongs to the genus Protoparvovirus in the subfamily Parvovirinae (Cotmore et al., 2014). The virus has a single-stranded DNA genome, encoding non-structural protein 1 (NS1), a putative structural protein 1 (VP1), a small hypothetical protein of 130 aa residues and a structural protein 2 (VP2), as demonstrated in a reference genome (GenBank accession number: NC_024888). Genome comparison and phylogenetic analysis have grouped human bufaviruses into three genotypes (Yahiro et al., 2014). Bufavirus has first been identified in faeces of children with acute diarrhoea in Burkina Faso, a landlocked country in West Africa, and found in one Tunisian child (Phan et al., 2012). Subsequently, the virus has been discovered sporadically in cases of diarrhoea in Bhutan (Yahiro et al., 2014), Finland (V€ ais€ anen et al., 2014, the Netherlands (Smits et al., 2014), Turkey (Altay et al., 2015), Thailand (Chieochansin et al., 2015) and China (Huang et al., 2015). The role of bufaviruses as an etiologic agent of human gastroenteritis has not been firmly established yet. Further metagenomic investigations show distinct lineages of bufavirus in wild shrews and non-human primates from Zambia (Sasaki et al., 2015), in Hungarian bats (Kemenesi et al., 2015) and pigs (Hargitai et al., 2016) and in Chinese rats (Yang et al., 2016). This report describes the identification and characterization of bufavirus in faeces of domestic pigs by a metagenomic approach.A total of 60 samples were collected from 6-10-week-old pigs, on five Austrian farms and one Hungarian farm (Fig. 1). The samples consisted of five diarrhoeic (semiliquid or liquid form) and five normal faeces per farm. One diarrhoeic and one normal sample from each farm were used for the metagenomic studies and all samples were used for subsequent epidemiological investigation. About 200 mg of faeces were diluted to 1 ml of water, vortexed vigorously for 1 min and centrifuged at 14 000 g for 10 min. Supernatant (400 µl) was treated with 400 U ml À1 DNase I (Ro...

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confidence: 62%

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confidence: 88%

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confidence: 99%

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Identification of a novel bufavirus in domestic pigs by a viral metagenomic approach

Schwarz

Ullman

Ahola

et al. 2016

Journal of General Virology

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“…Our recent pilot study on fecal virome analysis of the Hungarian bat fauna provided new insight into viral diversity, providing evidence of novel astroviruses and bufaviruses in M. schreibersii (Kemenesi et al, 2014(Kemenesi et al, , 2015. To further explore the ecological role of these common bats as virus reservoirs we involved additional geographical locations in our surveys.…”

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confidence: 99%

Candidate new rotavirus species in Schreiber's bats, Serbia

Bányai

Kemenesi

Budinski

et al. 2017

Infection, Genetics and Evolution

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The genus Rotavirus comprises eight species designated A to H and one tentative species, Rotavirus I. In a virus metagenomic analysis of Schreiber's bats sampled in Serbia in 2014 we obtained sequences likely representing novel rotavirus species. Whole genome sequencing and phylogenetic analysis classified the representative strain into a tentative tenth rotavirus species, we provisionally called Rotavirus J. The novel virus shared a maximum of 50% amino acid sequence identity within the VP6 gene to currently known members of the genus. This study extends our understanding of the genetic diversity of rotaviruses in bats.

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“…BuV-like viruses have been found in wild and captive non-human primates as well as in swine, shrews, rats, bats and fur seals46121314151617. The detection of these viruses in sera of rhesus monkeys in the USA, and in the spleen of wild baboons and shrews in Zambia, suggests that BuV-like viruses may cause systemic infections1213.…”

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Epidemiology of two human protoparvoviruses, bufavirus and tusavirus

Väisänen

Paloniemi

Kuisma

et al. 2016

Sci Rep

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Two human parvoviruses were recently discovered by metagenomics in Africa, bufavirus (BuV) in 2012 and tusavirus (TuV) in 2014. These viruses have been studied exclusively by PCR in stool and detected only in patients with diarrhoea, although at low prevalence. Three genotypes of BuV have been identified. We detected, by in-house EIA, BuV1-3 IgG antibodies in 7/228 children (3.1%) and 10/180 adults (5.6%), whereas TuV IgG was found in one child (0.4%). All children and 91% of the adults were Finnish, yet interestingly 3/6 adults of Indian origin were BuV-IgG positive. By competition EIA, no cross-reactivity between the BuVs was detected, indicating that the BuV genotypes represent distinct serotypes. Furthermore, we analysed by BuV qPCR stool and nasal swab samples from 955 children with gastroenteritis, respiratory illness, or both, and found BuV DNA in three stools (0.3%) and for the first time in a nasal swab (0.1%). This is the first study documenting the presence of BuV and TuV antibodies in humans. Although the seroprevalences of both viruses were low in Finland, our results indicate that BuV infections might be widespread in Asia. The BuV-specific humoral immune responses appeared to be strong and long-lasting, pointing to systemic infection in humans.

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Genetic diversity and recombination within bufaviruses: Detection of a novel strain in Hungarian bats

Cited by 37 publications

References 17 publications

Identification of a novel bufavirus in domestic pigs by a viral metagenomic approach

Identification of a novel bufavirus in domestic pigs by a viral metagenomic approach

Candidate new rotavirus species in Schreiber's bats, Serbia

Epidemiology of two human protoparvoviruses, bufavirus and tusavirus

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