Genetic Diversity and Quantification of Human Adenoviruses and JC Polyomaviruses in Wastewater Samples

Macena, Lorena da Graça Pedrosa de; Vieira, Carmen Baur; Maranhão, Adriana Gonçalves; Ferreira, Fernando César; Lemos, Elba Regina Sampaio de; Miagostovich, Marize Pereira

doi:10.26502/ijpaes.202125

Cited by 2 publications

(2 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Moreover, this study revealed the predominance of an HAdV sequence (90%) that had the closest relationship to a Brazilian HAdV, which could suggest it being an imported strain. A recent study conducted in Barra da Tijuca at the west coast of Rio de Janeiro in Brazil, revealed the highest prevalence of HAdV-F species (44%) in hospital sewage among the other detected species (B and D) [41]. Moreover, the growing requirements of technology and innovation in Saudi Arabia has led expatriates to represent about a quarter of the total population, which is associated with imported viral strains from foreign countries [42].…”

Section: Discussionmentioning

confidence: 99%

Human Adenovirus Detection and Genetic Characterization in Irrigation Water from the Riyadh Region, Saudi Arabia

Alkathiri,

Eifan,

Hanif

et al. 2023

Water

View full text Add to dashboard Cite

Adenoviruses are considered one of the most persistent enteric pathogens that can penetrate entire aquifer matrices. An ongoing monitoring of irrigation water is essential to mitigate potential public health risks. We investigated the prevalence of human adenoviruses (HAdV) in the groundwater discharge basins of Aluraiji (AW-DB) and Diriyah (DW-DB) and in the irrigation water of Al Harriq (H-IW) and Al Hayer (AH-IW) from January to December 2021. The meteorological impact (temperature, relative humidity, and wind speed) on HAdV prevalence and molecular diversity was investigated by targeting a selected region of the Hexon gene. The samples were concentrated using the polyethylene glycol precipitation (PEG) method. HAdVs were detected using PCR and sequenced by Sanger sequencing, and phylogenetic analysis was performed. The highest HAdV prevalence was recorded in H-IW, AH-IW, and DW-DB (100%). HAdV sequences were found to be closely related to species F (type 41) with a predominance of the 00-2B64 sequence (86.4%). Phylogenetic analysis depicted a close relationship of this study’s isolate 00-2B64 to a Brazilian and Saudi isolate, whereas 08-2B64 was found to be related to a sequence from an AnNazim landfill (LF1; d = 0.00) from Riyadh, Saudi Arabia. A high HAdV prevalence was recorded at a temperature range of 19–28 °C, wind speed was recorded at a range of (16–20 km/h), and relative humidity was recorded at a range of (15–25%). Meteorological variations exhibited no significant effect on the prevalence of HAdVs. The findings provided data on HAdV prevalence and predominant species in the irrigation water of Riyadh, Saudi Arabia and presented information regarding the environmental effects of HAdV persistence. In public health contexts, this will help in the planning of disease control strategies.

show abstract

Section: Discussionmentioning

confidence: 99%

Human Adenovirus Detection and Genetic Characterization in Irrigation Water from the Riyadh Region, Saudi Arabia

Alkathiri,

Eifan,

Hanif

et al. 2023

Water

View full text Add to dashboard Cite

show abstract

“…Hamza and Hamza, 2018 found high prevalence about 73% for HPyV in wastewater samples. There was a little difference in detection rate of HPyVs obtained byMacena et al, (2021) where they detected high percentage with 90% of JC Polyomaviruses in wastewater samples at Brazil and this could be as a result of the small number of samples that were collected which limits the study results. High prevalence HPyV in stool samples are remarkable during the current study in contrary the other study done byYu et al, (2012) where they found only 56% in Diarrhea samples.…”

mentioning

confidence: 84%

Wastewater and clinical based epidemiology for viral surveillance in urban sewage and clinical samples from Egypt

Shaheen,

Ahmed,

Elmahdy

2024

Preprint

View full text Add to dashboard Cite

The release of inadequately treated wastewater, containing human viruses, into the water environment or agricultural use represent a major problem in public health. In this study we evaluated the presence of polyomavirus (HPyV), papillomavirus (HPV), hepatitis A virus (HAV), and hepatitis E virus (HEV) in urban sewage and among children with acute gastroenteritis by real time PCR. The seasonal distribution in wastewater and viral removal by wastewater treatment process were also evaluated. HPyV, HPV, HAV, and HEV, were detected in 68%, 39.6% 42.4%, and 33.3% of the raw sewage and in 21%, 9.4%, 18.7%, and 0% of the treated sewage samples. Among the 200 children with acute gastroenteritis, HPyV, HPV, HAV, and HEV were detected in 72.5%, 50%, 13%, and 5% of stool samples tested, respectively. HPyV was more prevalent in both environmental and clinical samples. The concentration of these viruses (HPyV, HPV, HAV, HEV) in raw sewage, treated sewage, and stool samples ranged from 1.30×101 GC/ml to 9.86×107 GC/ml with a mean concentration of 3.62 ×106, from 0 GC/ml and 2.15×105 GC/ml with a mean concentration of 4.03×103, and from 1.40×101 to 9.85×107 GC/g with a mean concentration of 4.05×106 GC/g, respectively. Examination of wastewater treatment process efficiency based on mean concentration values at entry and exit observed an overall reduction of 49.5%, 47.9%, 41.2%, 100%, for HPyV, HPV, HAV, and HEV respectively. This study showed the benefit of environmental monitoring as an additional tool to investigate the epidemiology of these viruses circulating in a given community.

show abstract

Genetic Diversity and Quantification of Human Adenoviruses and JC Polyomaviruses in Wastewater Samples

Cited by 2 publications

References 17 publications

Human Adenovirus Detection and Genetic Characterization in Irrigation Water from the Riyadh Region, Saudi Arabia

Human Adenovirus Detection and Genetic Characterization in Irrigation Water from the Riyadh Region, Saudi Arabia

Wastewater and clinical based epidemiology for viral surveillance in urban sewage and clinical samples from Egypt

Contact Info

Product

Resources

About