Genetic diversity and population structure of bighead carp (Hypophthalmichthys nobilis) from the middle and lower reaches of the Yangtze River revealed using microsatellite markers

Zhu, Wenbin; Fu, Jianjun; Luo, Ming; Wang, Lanmei; Wang, Pan; Liu, Qigen; Dong, Zaijie

doi:10.1016/j.aqrep.2022.101377

Cited by 8 publications

(7 citation statements)

References 57 publications

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“…Notably, this may reflect the current state of differentiation of all populations and the lack of time for loss of genetic diversity following the census decline. However, N e values may have been varying and fluctuating in different parts of Guangxi due to different ecological characteristics, mating system dynamics, gene flow, and genetic mixing [ 35 ].…”

Section: Discussionmentioning

confidence: 99%

Genetic diversity and population structure of Bellamya purificata in Guangxi

Yuan,

Li,

Zhou

et al. 2024

PLoS ONE

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Bellamya purificata is an important medicinal value and economically farmed species in China. However, because little is known about the genetic characteristics of this species, the utilization of high-quality germplasm resources is hindered. The study examined the genetic differentiation between, and the structure of 12 B. purificata populations in Guangxi using 7 microsatellite DNA markers. High genetic diversity occurred in each population, with mean observed heterozygosity 0.655 and a mean expected heterozygosity 0.832. Analysis of molecular variance reveals genetic diversity to be greater within (95.2%) than among populations (4.8%). Genetic differentiation between populations is weak (Fst = 0.048, P < 0.001), with mixing of genetic clusters prevalent at the level of the individual. Genetic flow exists between populations (Nm = 3.084–11.778), with Longshui and Guilin populations exchanging frequently. A Mantel test reveals a low correlation between geographic and genetic distances (r = 0.2482, P < 0.071), suggesting that dispersal between neighboring populations facilitates population exchange. No significant heterozygosity excess was observed for any population (P > 0.05), indicating a lack of recent genetic bottlenecks. The results provide important genetic information for B. purificata, and data for potential germplasm discovery and aquaculture development.

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Section: Discussionmentioning

confidence: 99%

Genetic diversity and population structure of Bellamya purificata in Guangxi

Yuan,

Li,

Zhou

et al. 2024

PLoS ONE

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“…Null alleles might be accounted for insufficient sampling 45 and variation of microsatellite flanking sequence 46 . Loss of heterozygosity might be accounted for migration, artificial selection, and inbreeding 47 , 48 , which was common in marine species such as Scylla paramamosain 49 – 51 , Pinctada margaritifera 52 , and Hypophthalmichthys nobilis 53 . Chen et al 54 used ten SSRs to investigate the effect of artificial selection on the genetic structure of two abalone lines and found a loss of heterozygosity ( Ho = 0.650 < He = 0.711).…”

Section: Discussionmentioning

confidence: 99%

Microsatellite markers reveal genetic diversity and population structure of Portunus trituberculatus in the Bohai Sea, China

Duan

Kang

Wan

et al. 2023

Sci Rep

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The swimming crab, Portunus trituberculatus, is one of the main aquaculture species in Chinese coastal regions due to its palatability and high economic value. To obtain a better understanding of the genetic diversity of P. trituberculatus in the Bohai Sea, the present study used 40 SSR loci to investigate the genetic diversity and population structure of 420 P. trituberculatus individuals collected from seven populations in the Bohai Sea. Genetic parameters revealed a low level of genetic diversity in the cultured population (SI = 1.374, He = 0.687, and PIC = 0.643) in comparison with wild populations (SI ≥ 1.399, He ≥ 0.692, and PIC ≥ 0.651). The genetic differentiation index (Fst) and gene flow (Nm) ranged from 0.001 to 0.060 (mean: 0.022) and 3.917 to 249.750 (mean: 31.289) respectively, showing a low differentiation among the seven populations of P. trituberculatus. Population structure analysis, phylogenetic tree, and principal component analysis (PCA) demonstrated that the seven groups of P. trituberculatus were divided into four subpopulations (K = 4), but the correlation between genetic structure and geographical distribution was not obvious. These results are expected to provide useful information for the fishery management of wild swimming crabs.

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“…1). O. hupensis samples were fed for one week and identi ed whether O. hupensis were infected with S. japonicum by observation of cercaria emergence from snails [27] . Thirty randomly selected negative snails from each sampling site were used in this study.…”

Section: Sources Of O Hupensismentioning

confidence: 99%

“…Six microsatellite DNA polymorphic loci were selected from previous microsatellite loci library (i.e., P82, P84, T4-22, T5-11, T5-13, T6-27) [27] , primer sequences and information are presented in Table 2. PCR ampli cation was performed.…”

Section: Gene Scanmentioning

confidence: 99%

The genetic diversity of Oncomelania hupensis robertsoni, intermediate hosts Schistosome japonicum in hilly regions of China using microsatellite markers

Song,

Wang,

et al. 2024

Preprint

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Background The elimination of schistosomiasis remains a challenging task, with current measures primarily focused on the monitoring and control of Oncomelania hupensis (O. hupensis) snail, the sole intermediate host Schistosome japonicum. Given the emerging, re-emerging, and persistent habitats of snails, a study on the genetic diversity of snails might be an essential. Objective The aims of this study were to (1) analyze the genetic diversity of O. hupensis robertsoni Yunnan strain (O. h. r. Yunnan strain) using microsatellite DNA markers; and (2) validate the applicability of previously identified microsatellite loci for O. hupensis in hilly regions. Methods A total of 17 populations of O. hupensis from Yunnan Province in China were selected for analysis of genetic diversity by 6 microsatellite DNA polymorphic loci (i.e., P82, P84, T4-22, T5-11, T5-13, T6-27). Results the number of alleles among populations ranged from 0 to 19, with an average of 5. The average ranges of expected (He) and observed (Ho) heterozygosity within populations were 0.506 to 0.761 and 0.443 to 0.792, respectively. The average fixation index within population ranged from − 0.801 to 0.211. The average polymorphic information content (PIC) within population ranged from 0.411 to 0.757, appearing polymorphic for all loci (all PIC > 0.5), except for P28 and P48. A total of 68 loci showed significant deviations from Hardy-Weinberg equilibrium (P < 0.05), and pairwise Fst values ranged from 0.051 to 0.379. The analysis of molecular variance indicated that 88% of the variation exists within snail populations, while 12% exists among snail populations. Phylogenetic trees and principal coordinate analysis revealed two distinct clusters within the snail population, corresponding to "Yunnan North" and "Yunnan South". Conclusion O. h. r. Yunnan strain exhibits a relatively high level of genetic differentiation, with variation chiefly existing within snail populations. All snail could be separated into two clusters. The microsatellite loci P82 and P84 may not be suitable for the classification study of O. hupensis in hilly regions. These findings provide the important information for monitoring and control of snail, and further genetic diversity studies on snail populations.

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Genetic diversity and population structure of bighead carp (Hypophthalmichthys nobilis) from the middle and lower reaches of the Yangtze River revealed using microsatellite markers

Cited by 8 publications

References 57 publications

Genetic diversity and population structure of Bellamya purificata in Guangxi

Genetic diversity and population structure of Bellamya purificata in Guangxi

Microsatellite markers reveal genetic diversity and population structure of Portunus trituberculatus in the Bohai Sea, China

The genetic diversity of Oncomelania hupensis robertsoni, intermediate hosts Schistosome japonicum in hilly regions of China using microsatellite markers

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