Genetic diversity and population structure in the Leishmania guyanensis vector Lutzomyia anduzei (Diptera, Psychodidae) from the Brazilian Amazon

Scarpassa, Vera Margarete; Figueiredo, Adrya da Silva Figueiredo; Alencar, Ronildo Baiatone

doi:10.1016/j.meegid.2015.02.007

Cited by 10 publications

(13 citation statements)

References 38 publications

(23 reference statements)

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“…longipalpis flight range is from 1 m to 500 m, but no more than 1,000 m around their breeding sites [ 86 ]. This flight range limitation would lead to rapid local population differentiation, due to genetic drift or to population fragmentation [ 43 , 44 , 51 ]. In Argentina, two specific Lu .…”

Section: Discussionmentioning

confidence: 99%

“…cruciata (Coquillett) [ 43 ], Lu . anduzei (Rozeboom) [ 44 ], Lu . olmeca olmeca (Vargas & Díaz-Nájera) [ 45 ] and old world sandfly species e.g.…”

Section: Introductionmentioning

confidence: 99%

“…Genetic diversity, genetic differentiation, and sandfly speciation have been associated with multiple factors, such as latitude or altitude, distance between populations, habitat modifications, anthropogenic landscape fragmentation, vegetation type, geographic barriers (rivers, mountains), host communities, and host species turnover. These factors reduce sandfly dispersal capacity thereby giving rise to isolated populations, loss of genetic diversity, and increasing differentiation among populations [ 20 , 39 – 41 , 43 , 44 , 46 , 48 – 51 ].…”

Section: Introductionmentioning

confidence: 99%

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Genetic diversity, phylogeography and molecular clock of the Lutzomyia longipalpis complex (Diptera: Psychodidae)

et al. 2018

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BackgroundThe Lutzomyia longipalpis complex has a wide but discontinuous distribution in Latin America, extending throughout the Neotropical realm between Mexico and northern Argentina and Uruguay. In the Americas, this sandfly is the main vector of Leishmania infantum, the parasite responsible for Visceral Leishmaniasis (VL). The Lu. longipalpis complex consists of at least four sibling species, however, there is no current consensus on the number of haplogroups, or on their divergence. Particularly in Argentina, there have been few genetic analyses of Lu. longipalpis, despite its southern expansion and recent colonization of urban environments. The aim of this study was to analyze the genetic diversity and structure of Lu. longipalpis from Argentina, and to integrate these data to re-evaluate the phylogeography of the Lu. longipalpis complex using mitochondrial markers at a Latin American scale.Methodology/Principal findingsGenetic diversity was estimated from six sites in Argentina, using a fragment of the ND4 and the 3´ extreme of the cyt b genes. Greatest genetic diversity was found in Tartagal, Santo Tomé and San Ignacio. There was high genetic differentiation of Lu. longipalpis in Argentina using both markers: ND4 (FST = 0.452, p < 0.0001), cyt b (FST = 0.201, p < 0.0001). Genetic and spatial Geneland analyses reveal the existence of two primary genetic clusters in Argentina, cluster 1: Tartagal, Santo Tomé, and San Ignacio; cluster 2: Puerto Iguazú, Clorinda, and Corrientes city. Phylogeographic analyses using ND4 and cyt b gene sequences available in GenBank from diverse geographic sites suggest greater divergence than previously reported. At least eight haplogroups (three of these identified in Argentina), each separated by multiple mutational steps using the ND4, are differentiated across the Neotropical realm. The divergence of the Lu. longipalpis complex from its most recent common ancestor (MRCA) was estimated to have occurred 0.70 MYA (95% HPD interval = 0.48–0.99 MYA).Conclusions/SignificanceThis study provides new evidence supporting two Lu. longipalpis genetic clusters and three of the total eight haplogroups circulating in Argentina. There was a high level of phylogeographic divergence among the eight haplogroups of the Lu. longipalpis complex across the Neotropical realm. These findings suggest the need to analyze vector competence, among other parameters intrinsic to a zoonosis, according to vector haplogroup, and to consider these in the design and surveillance of vector and transmission control strategies.

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Section: Discussionmentioning

confidence: 99%

“…cruciata (Coquillett) [ 43 ], Lu . anduzei (Rozeboom) [ 44 ], Lu . olmeca olmeca (Vargas & Díaz-Nájera) [ 45 ] and old world sandfly species e.g.…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Genetic diversity, phylogeography and molecular clock of the Lutzomyia longipalpis complex (Diptera: Psychodidae)

et al. 2018

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“…Phylogenetics is an efficient approach to assessing genetic structure and diversity of a species population. Studying the spatial genetic structure of a species is crucial to knowing current population genetic processes and predicting the potential of populations to respond to selection and divergent environmental conditions (De Jong et al ., ; Scarpassa et al ., ). Phylogenetic methods have been widely applied to various kinds of insects, such as the grass‐cutting ant Acromyrmex striatus (Cristiano et al ., ), Amphiareus obscuriceps (Hemiptera: Anthocoridae) (Zhang et al ., ), and some New Zealand and Mediterranean cicadas (Arensburger et al ., ; Pinto‐Juma et al ., ; Marshall et al ., , , ), and has provided solid evidence for past biogeographical events.…”

Section: Introductionmentioning

confidence: 97%

Morphological variation, genetic differentiation and phylogeography of the East Asia cicada Hyalessa maculaticollis (Hemiptera: Cicadidae)

Liu

Qiu

Wang

et al. 2017

Systematic Entomology

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The cicada Hyalessa maculaticollis is widely distributed in East Asia, and is noted for its great morphological variability. The variation in this species and its allies has been a long-standing controversy. The population differentiation, genetic structure and phylogeography of this species are explored based on morphological observations, mitochondrial and nuclear DNA analyses, and comparison of the calling song structure of males. Our results reveal that the abundant intraspecific morphological variations are consistent with high levels of genetic divergence in this species, but incongruence between the morphological variations and genetic divergence is found in a few lineages. Phylogenetic and network analyses indicate that H. maculaticollis is composed of two major lineages -China and Japan. The East China Sea (ECS) land bridge acted as a dispersal corridor for H. maculaticollis during the glacial period. The climatic oscillations in the Pleistocene and the terrain structure of East Asia influenced population differentiation. The divergence time between the two sides of the East China Sea is estimated to be ∼1.05 (95% CI = 0.80-1.30) Ma, which was about the same period during which the sea level increased rapidly during the 'Ryukyu Coral Sea Stage' (0.2-1.3 Ma). Populations of H. maculaticollis are structured phylogeographically, with the China populations differentiated into a greater number of highly structured haplogroups. Qinling Mountains and the mountainous regions around the Sichuan Basin are presumed to have been major refugia for H. maculaticollis in glacial periods, and a recent population expansion has been detected for populations distributed in the area to the north of Qinling Mountains. The high degree of haplotype and nucleotide diversity shown in East China populations suggests that the flat terrain with low-altitude hills are suitable for the survival of H. maculaticollis. The species H. fuscata, treated as an independent species from H. maculaticollis by some researchers based on acoustic analyses of the calling song structure, is confirmed to be a junior synonym of H. maculaticollis based on the results of our analyses of morphological variation, calling song structure and acoustic playback experiments. Past geological events and climatic oscillations have played important roles in forming the contemporary genetic diversity Correspondence: Cong Wei, Key Laboratory of Plant Protection Resources and Pest Management, Ministry of Education, Entomological Museum,

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“…In the last years, as genetic molecular markers became available, the number of studies on population genetics and evolutionary genetics in sandfly species has significantly increased [44][45][46][47][48][49], and the results have revealed large intra-population genetic variation, genetically populations structured, genetic lineages and cryptic species complexes. In the case of vector species, the knowledge of the genetic structure of populations and the processes responsible for the differentiation distribution is important for the identification of the disease transmission heterogeneity patterns.…”

Section: Distribution Biological Aspects and Population Genetics Of mentioning

confidence: 99%

Speciation in the Leishmania guyanensis Vector Lutzomyia umbratilis (Diptera: Psychodidae) from Northern Brazil — Implications for Epidemiology and Vector Control

Scarpassa¹,

Alencar²

2015

An Overview of Tropical Diseases

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This chapter starts with a brief mention of the Leishmania species and sandflies vectors that occur in the Neotropical region, especially in the "razilian "mazon. The main focus of this chapter is a review of the taxonomic, biologic and epidemiologic studies conducted in Lutzomyia umbratilis, the main vector of Leishmania guyanensis in the northern region of "razil. We associated these data with the population genetics studies carried out in this sandfly vector by our research team. The genetic studies were made with six samples of L. umbratilis from the central region of the "razilian "mazon, using a large fragment , bp of the mitochondrial DN" COI gene. "lso, another study was conducted in these samples using the DN" barcode region. The results revealed rather high levels of genetic variability for all samples analyzed and a pronounced genetic differentiation between samples from both banks of the Negro and "mazon rivers. The degree of differentiation found may reflect the presence of distinct species within L. umbratilis, suggesting that the "mazon and Negro rivers may be acting as effective barriers, preventing gene flow between populations living on the two sides. These findings have important implications for epidemiology, especially regarding vector competence, which is vital information for surveillance and vector control strategies. Furthermore, this diversification process of L. umbratilis represents an interesting example for speciation studies.

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Genetic diversity and population structure in the Leishmania guyanensis vector Lutzomyia anduzei (Diptera, Psychodidae) from the Brazilian Amazon

Cited by 10 publications

References 38 publications

Genetic diversity, phylogeography and molecular clock of the Lutzomyia longipalpis complex (Diptera: Psychodidae)

Genetic diversity, phylogeography and molecular clock of the Lutzomyia longipalpis complex (Diptera: Psychodidae)

Morphological variation, genetic differentiation and phylogeography of the East Asia cicada Hyalessa maculaticollis (Hemiptera: Cicadidae)

Speciation in the Leishmania guyanensis Vector Lutzomyia umbratilis (Diptera: Psychodidae) from Northern Brazil — Implications for Epidemiology and Vector Control

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