Genetic diversity and population structure of Leucaena leucocephala (Lam.) de Wit genotypes using molecular and morphological attributes

Rajarajan, K.; Uthappa, A. R.; Handa, A. K.; Chavan, S. B.; Vishnu, R.; Shrivastava, Anish; Rana, Maneet; Sahu, S; Kumar, Neeraj; Singh, A. K.; Ahmed, Shahid

doi:10.1007/s10722-021-01203-7

Cited by 6 publications

(1 citation statement)

References 29 publications

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“…Earlier work carried out in India by Rajarajan et al [16] in studying genetic variability among 21 L. leucocephala accessions using simple SSR markers resulted in 82.57 % of polymorphism among the traits. Similar kind of analyzing genetic variations among the accessions of other pulpwood species like Casuarina [17,5], Eucalyptus [18][19][20] and Populus [21,22] were already well documented.…”

Section: Number Of Bands Polymorphism and Provenances Distinguished W...mentioning

confidence: 99%

Genetic Assessment of Leucaena leucocephala (Lam.) de Wit Provenances by Using ISSR Markers

P. Chezhian,

Prasath,

K. Jayakumar

et al. 2024

AJB2T

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Inter Simple Sequence Repeats (ISSR) technique was deployed for the estimation of genetic diversity in18 Leucaena leucocephala provenances. Seeds of these provenances were collected from across the country and planted at a single location in South India. To estimate the genetic diversity/ polymorphism, DNA was extracted through Cetyl Trimethyl Ammonium Bromide (CTAB) method, visualized and analyzed the DNA bands on UV trans- illuminator gel doc (α-imager 3.2). Totally ten ISSR primers were used in this study. Among them, eight ISSR microsatellites showed a high degree of polymorphism in all the eighteen leucaena provenances used in our study. These primers successfully amplified a total of 645 bands, of which 577 bands (89 %) showed polymorphism. On average, 14.92 bands per accession were obtained and 68.28 % provenances were distinguished through these primers. The ISSR primer T(GT)9 was able to distinguish 100 % of the L. luecocephalla provenances. Cluster analysis based on the genotypic similarities assembled the 18 leucaena accessions in to three clusters. The dendrogram generated based on the 8 ISSR primers distinctively demarcated many of the L. leucocephalla accessions into different clusters indicating vast genetic diversity among the provenances. The high degree of genetic variability demonstrated by the ISSR molecular markers in L. leucocephalla can be used as a tool for assessing genetic diversity and in future leucaena genetic improvement programs.

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Section: Number Of Bands Polymorphism and Provenances Distinguished W...mentioning

confidence: 99%

Genetic Assessment of Leucaena leucocephala (Lam.) de Wit Provenances by Using ISSR Markers

P. Chezhian,

Prasath,

K. Jayakumar

et al. 2024

AJB2T

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Genetic diversity and population structure of Azadirachta indica using simple sequence repeat (SSR) markers for genetic resource conservation and utilization

Rajarajan,

Sahu,

Handa

et al. 2024

Genet Resour Crop Evol

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Understanding the genetic determinants and population structure of Pongamia pinnata (L.) Pierre for oil yield and its properties using transcriptome derived SSR markers

Rajarajan¹,

Sakshi²,

Rana³

et al. 2022

Plant Physiol. Rep.

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Genetic diversity and population structure of Leucaena leucocephala (Lam.) de Wit genotypes using molecular and morphological attributes

Cited by 6 publications

References 29 publications

Genetic Assessment of Leucaena leucocephala (Lam.) de Wit Provenances by Using ISSR Markers

Genetic Assessment of Leucaena leucocephala (Lam.) de Wit Provenances by Using ISSR Markers

Genetic diversity and population structure of Azadirachta indica using simple sequence repeat (SSR) markers for genetic resource conservation and utilization

Understanding the genetic determinants and population structure of Pongamia pinnata (L.) Pierre for oil yield and its properties using transcriptome derived SSR markers

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