Genetic Diversity and Population Structure of Potato Germplasm in RDA-Genebank: Utilization for Breeding and Conservation

Abstract

Potato (Solanum tuberosum L.) is an important staple food and economic crop in many countries. It is of critical importance to understand the genetic diversity and population structure for effective collection, conservation, and utilization of potato germplasm. Thus, the objective of the present study was to investigate the genetic diversity and population structure of potato germplasm conserved in the National Agrobiodiversity Center (NAC) of South Korea to provide basic data for future preservation and breeding of potato genetic resources. A total of 24 simple sequence repeat (SSR) markers were used to assess the genetic diversity and population structure of 482 potato accessions. A total of 257 alleles were detected, with an average of 10.71 alleles per locus. Analysis of molecular variance showed that 97% of allelic diversity was attributed to individual accessions within the population, while only 3% was distributed among populations. Results of genetic structure analysis based on STRUCTURE and discriminant analysis of principal components revealed that 482 potato accessions could be divided into two main subpopulations. Accessions of subpopulation 1 mainly belonged to cultivars and breeding lines. Accessions of subpopulations 2 basically corresponded to wild relatives of potatoes. Results of this study provide useful information for potato improvement and conservation programs, although further studies are needed for a more accurate evaluation of genetic diversity and phenotypic traits of potatoes.

Simple Sequence Repeats-Based Genetic Characterization and Varietal Identification of Potato Varieties Grown in Pakistan

“…SSR markers are extensively utilized for DNA fingerprinting [15], parental investigations [16], genotype relocations [13] and genetic diversity analysis [12] in potatoes. According to the current status, many researchers are working on SSR markers' advancement and adaptability across potato varieties to determine their genetic diversity and achieve varietal identification [17].…”

“…Furthermore, SSR markers are extensively utilized for DNA fingerprinting [15], parental investigation [16], genotype relocations [13], and genetic diversity analysis [12] in potatoes. According to the current status of research, many researchers are working on SSR markers' advancement and adaptability across potato varieties with respect to determining genetic diversity and varietal identification [17] The goal of the current study on potatoes is to provide basic information for the breeding of genetic resources and conservation, to explore the genetic relationships and diversity among different potato cultivars, and to perform varietal identification using SSR markers. Although numerous studies have already been reported worldwide regarding potato DNA fingerprinting, no such data is available in Pakistan to date.…”

Genotyping-by-sequencing and weighted gene co-expression network analysis of genes responsive against Potato virus Y in commercial potato cultivars

“…revealed that clustering of the cultivars tend to be based on their geographical origin [44]. We utilized DAPC, PCA, NJ cluster analysis, and MSN analysis to truly understand the genetic structure of these cultivars.…”

Application of Molecular Markers in Vegetable Improvement : A Review

“…The level of polymorphism is based on insertion, deletion, duplication, translocation and point mutations whereas they did not affect the activity of genes (Ghareyazie et al, 1995). Properties desirable for ideal DNA markers (Jiang, 2013;Joshi et al, 2011) Genetic diversity, genetic relationships and population structure improvement, DNA fingerprinting and molecular variance, genetic variation, evolutionary relatedness in Capsicum (Lee et al, 2021;La Cruz et al, 2020;Singh et al, 2020). Sharmin et al (2018) also used SSR markers for the assessment of Genetic variability and genetic diversity in Brinjal.…”