Genetic diversity and population structure of the serpentine endemic Ni hyperaccumulator Alyssum lesbiacum

Adamidis, George C.; Dimitrakopoulos, Panayiotis G.; Manolis, Apostolos; Papageorgiou, Aristotelis C.

doi:10.1007/s00606-014-1047-3

Cited by 9 publications

(6 citation statements)

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“…Perennial and outcrossing plant species with similar geographical expansion usually exhibit higher levels of diversity within populations, when examined with the same type of genetic markers (e.g. ADAMIDIS et al, 2014;NYBOM, 2004). However, peripheral populations of plant species with a patchy distribution type in a fragmented landscape often have low genetic diversity (e.g.…”

Section: Discussionmentioning

confidence: 99%

Genetic diversity and spatial structure of the imperiled European population of Malus trilobata in Greece

et al. 2021

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Malus trilobata, is a rare tree species occurring in several small and disjunct populations in the eastern part of the Mediterranean basin. The main European population is found in the region of Evros (NE Greece) and is divided in five distinct subpopulations following the species geographical pattern. The genetic diversity of approximately the entire population (69 trees) was analyzed using nuclear microsatellite and random genomic markers. Polymorphism was discovered in 29 out of 45 genomic marker loci (64.44%), while for nuclear microsatellite markers, all three loci were polymorphic with an average of 3.75 alleles per locus. Our results unraveled a specific grouping pattern for both markers. Both genetic markers exhibited relatively low genetic diversity which is in accordance with the prevalent perception that species with fragmented distributions tend to have low genetic diversity, while the differentiation among individuals, revealed a patchy pattern among small groups of trees separated by roads, firebreaks or distance. These results indicate a high genetic fragmentation level for the main European population of M. trilobata, while the presence of roads, firebreaks, conifer plantations and agricultural land, seem to function as potential barriers to gene flow. Consequently, and since it is well-documented that bees hesitate to change foraging patches, as long as their food is abundant, the observed genetic differentiation patterns could be partially attributed to the foraging and flight behavior of bees, which are the main pollinators of the species. The low levels of available genetic diversity combined with the small overall population and repeated events of forest fires inside the M. trilobata distribution, perils the survival of the species and imposes the necessity for a thoroughly organized conservation strategy.

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Section: Discussionmentioning

confidence: 99%

Genetic diversity and spatial structure of the imperiled European population of Malus trilobata in Greece

et al. 2021

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“…Both sites supported large populations of O. lesbiaca [ 19 ]. Loutra population is genetically differentiated from the populations grown at the central part of the island in which Ampeliko population is included [ 17 ].…”

Section: Methodsmentioning

confidence: 99%

“…The large portion of hyperaccumulating species (85–90%) are serpentine-endemic ones [ 14 ]. Studies on serpentine endemic hyperaccumulating species have been demonstrated genetically distinct populations, with a relatively high genetic heterogeneity within the same population, and an important effect of geographic isolation on the genetic differentiation of populations [ 15 , 16 , 17 ]. In addition, field studies showed populations with different hyperaccumulating capacity related to soil nickel levels in the habitat of their origin for various Odontarrhena species [ 16 , 18 , 19 ], demonstrating the influence of small-scale serpentine-soil heterogeneity in determining intraspecific variation in foliar nickel concentration [ 16 , 19 ], as well as in other plant functional traits [ 7 ].…”

Section: Introductionmentioning

confidence: 99%

“…The aim of the present study was to examine nitro-oxidative stress in the root system of the Ni hyperaccumulator O. lesbiaca grown in a soil filled rhizotron system. For this study, two geographically distant serpentine habitats on Lesbos were selected that were differed in their soil Ni content [ 19 ] and they support genetically different populations of O. lesbiaca [ 17 ]. Our goal was to determine how excess Ni modifies the root system architecture of O. lesbiaca originating from the two different habitats.…”

Section: Introductionmentioning

confidence: 99%

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Different Nitro-Oxidative Response of Odontarrhena lesbiaca Plants from Geographically Separated Habitats to Excess Nickel

et al. 2020

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Odontarrhena lesbiaca is an endemic species to the serpentine soils of Lesbos Island (Greece). As a nickel (Ni) hyperaccumulator, it possesses an exceptional Ni tolerance; and it can accumulate up to 0.2–2.4% Ni of its leaves’ dry weight. In our study, O. lesbiaca seeds from two geographically separated study sites (Ampeliko and Loutra) were germinated and grown on control and Ni-containing (3000 mg/kg) soil in a rhizotron system. Ni excess induced significant Ni uptake and translocation in both O. lesbiaca ecotypes and affected their root architecture differently: plants from the Ampeliko site proved to be more tolerant; since their root growth was less inhibited compared to plants originated from the Loutra site. In the roots of the Ampeliko ecotype nitric oxide (NO) was being accumulated, while the degree of protein tyrosine nitration decreased; suggesting that NO in this case acts as a signaling molecule. Moreover, the detected decrease in protein tyrosine nitration may serve as an indicator of this ecotype’s better relative tolerance compared to the more sensitive plants originated from Loutra. Results suggest that Ni hypertolerance and the ability of hyperaccumulation might be connected to the plants’ capability of maintaining their nitrosative balance; yet, relatively little is known about the relationship between excess Ni, tolerance mechanisms and the balance of reactive nitrogen species in plants so far.

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“…This suggested pre-adaptive capacity to accumulate Ni when growing on ultramafics, and repeated colonization events on distinct outcrops in different regions and species complexes. However, most molecular studies on Ni-accumulating plants of this genus have focused on single obligate serpentine endemics, such as O. bertolonii [19] or O. lesbiaca [20], whilst none have examined the population genetic structure of entire species complexes including obligate and facultative serpentine taxa from ultramafic and non-ultramafic soils patchily distributed in geologically variable regions. Notwithstanding, such complexes provide a unique opportunity to examine population differentiation within and among species, and to shed light on the evolutionary dynamics of edaphic specialization, ecotypic variation, and speciation.…”

Section: Introductionmentioning

confidence: 99%

Population Genetics of Odontarrhena (Brassicaceae) from Albania: The Effects of Anthropic Habitat Disturbance, Soil, and Altitude on a Ni-Hyperaccumulator Plant Group from a Major Serpentine Hotspot

Coppi

Baker

Bettarini

et al. 2020

Plants

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Albanian taxa and populations of the genus Odontarrhena are most promising candidates for research on metal tolerance and Ni-agromining, but their genetic structure remains unknown. We investigated phylogenetic relationships and genetic differentiation in relation to distribution and ploidy of the taxa, anthropic site disturbance, elevation, soil type, and trace metals at each population site. After performing DNA sequencing of selected accessions, we applied DNA-fingerprinting to analyze the genetic structure of 32 populations from ultramafic and non-ultramafic outcrops across Albania. Low sequence divergence resulted in poorly resolved phylograms, but supported affinity between the two diploid serpentine endemics O. moravensis and O. rigida. Analysis of molecular variance (AMOVA) revealed significant population differentiation, but no isolation by distance. Among-population variation was higher in polyploids than in diploids, in which genetic distances were lower. Genetic admixing at population and individual level occurred especially in the polyploids O. chalcidica, O. decipiens, and O. smolikana. Admixing increased with site disturbance. Outlier loci were higher in serpentine populations but decreased along altitude with lower drought and heat stress. Genetic variability gained by gene flow and hybridization at contact zones with “resident” species of primary ultramafic habitats promoted expansion of the tetraploid O. chalcidica across anthropogenic sites.

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Genetic diversity and population structure of the serpentine endemic Ni hyperaccumulator Alyssum lesbiacum

Cited by 9 publications

References 48 publications

Genetic diversity and spatial structure of the imperiled European population of Malus trilobata in Greece

Genetic diversity and spatial structure of the imperiled European population of Malus trilobata in Greece

Different Nitro-Oxidative Response of Odontarrhena lesbiaca Plants from Geographically Separated Habitats to Excess Nickel

Population Genetics of Odontarrhena (Brassicaceae) from Albania: The Effects of Anthropic Habitat Disturbance, Soil, and Altitude on a Ni-Hyperaccumulator Plant Group from a Major Serpentine Hotspot

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