Genetic Diversity and Population Structure Analysis in Indian Mustard Germplasm Using Phenotypic Traits and SSR Markers

Sharma, D. K.; Nanjundan, J.; Singh, Lal; Parmar, Nehanjali; Singh, K. H.; Verma, Kumar Sambhav; Thakur, Ajay Kumar

doi:10.1007/s11105-022-01339-5

Cited by 10 publications

(16 citation statements)

References 37 publications

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“…Genetic diversity is usually not analyzed using phenotypic or molecular markers alone, but rather a combination of phenotypic and molecular markers, etc. are analyzed along with each other, as reported in other crops [12][13][14].…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity and population structure of sweet potato landraces based on phenotypic traits, carotenoid content, and SSR molecular markers

Chen

Tang

Zhang

et al. 2023

Preprint

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Germplasm resources are an important basis for genetic breeding, and the study of genetic diversity is beneficial to the discovery and improvement of germplasm resources. In this study, 132 local germplasm of sweet potato were used as test materials, and 20 phenotypic traits were analyzed for genetic diversity using distribution frequency, coefficient of variation, and Shannon's diversity index; carotenoid contents in sweet potato tubers were measured by HPLC, and their genetic diversity was analyzed using the coefficient of variation, mean, and standard deviation. Meanwhile, the genetic diversity, as well as population structure, were analyzed based on 10 pairs of SSR molecular markers using Nei's genetic diversity index and Shannon's diversity index. The results showed that the 132 sweet potato landraces had high genetic diversity based on phenotypic traits, SSR molecular markers, and carotenoid content, respectively; based on population structure analysis, the 132 sweet potato landraces could be divided into five subgroups. Analysis of molecular variation (AMOVA) showed that 84% of the variation existed within populations and 16% between populations. This study provides a basis for the selection of parental material for the genetic breeding improvement of sweet potato and for the mining of superior genes.

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Section: Introductionmentioning

confidence: 99%

Genetic diversity and population structure of sweet potato landraces based on phenotypic traits, carotenoid content, and SSR molecular markers

Chen

Tang

Zhang

et al. 2023

Preprint

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“…1 Mustard is a worldwide significant oilseed crop; therefore, studying the genetic diversity of different germplasm collections is crucial for its genetic improvement. 2 It is an important edible oil crop, broadly includes rapeseed and mustards which are used as a food, especially in the arid and semi-arid areas. 3,4 In addition, rapeseed is used to substitute for fossil diesel fuel.…”

Section: Introductionmentioning

confidence: 99%

“…The major components in rapeseed oil are unsaturated fatty acids (mainly oleic, erucic, and linoleic acid). 2,5 The majority of the mustard and rapeseed cultivars grown in Ethiopia contain high levels of erucic acid. 6 Because of this, Ethiopian mustard and rapeseed quality breeding has focused on altering the fatty acid contents of seed oil to create novel genotypes with different oil characteristics.…”

Section: Introductionmentioning

confidence: 99%

“…‟Gomenzer” or Ethiopian mustard (Brassica carinata) is the potential oilseed crop, which is mostly grown in the Ethiopia highlands 1 . Mustard is a worldwide significant oilseed crop; therefore, studying the genetic diversity of different germplasm collections is crucial for its genetic improvement 2 . It is an important edible oil crop, broadly includes rapeseed and mustards which are used as a food, especially in the arid and semi‐arid areas 3,4 .…”

Section: Introductionmentioning

confidence: 99%

“…It is mainly composed of fatty acids such as palmitic (16:0), stearic (18:0), oleic (18:1), linoleic (18:2), linolenic (18:3), Eicosenoic (20:1), and erucic (22:1) acids. The major components in rapeseed oil are unsaturated fatty acids (mainly oleic, erucic, and linoleic acid) 2,5 . The majority of the mustard and rapeseed cultivars grown in Ethiopia contain high levels of erucic acid 6 .…”

Section: Introductionmentioning

confidence: 99%

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Development of non‐destructive models to predict oil content and fatty acid composition of Gomenzer (Ethiopian mustard) using near‐infrared reflectance spectroscopy

et al. 2023

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Background ‟Gomenzer” or Ethiopian mustard is the potential oilseed crop that is economically important. Ethiopian mustard and rapeseed quality breeding has focused on altering the fatty acid contents of seed oil to create novel genotypes with different oil characteristics. The goal of this research was to establish calibration equations' using a method called near‐infrared reflectance spectroscopy with modified partial least squares (MPLS) regression. Result The spectra of 180 mustard samples were collected and their oil and fatty acid compositions were determined by n‐hexane extraction and GC–MS methods respectively. With 130 samples, calibration equations were developed for oil and fatty acid compositions. All developed equation had an acceptable value of R 2c, RPDc, and also had suitable 1‐VR values. Prediction of an external validation with 50 datasets revealed a blameless correlation between reference values and NIRS values based on the R 2v in prediction, SEP, and the ratio of SD to SEP (RPDv) for oil, oleic, linoleic, linolenic, and erucic acids. These had suitable values of RPDv and R 2v, with the range of 2.0 to 8.8 and 0.79 to 0.99 respectively. Conclusion The developed models gave meaningful quantitative data, however, the equations generated for palmitic, stearic, and eicosenoic acids were insufficient for data analysis. Hence, the developed NIRS model, which was satisfactory, could be used to assess and evaluate the oil and fatty acid content for the unknown mustard samples on a regular basis in the oilseed breeding quality program.

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Innovations in Assessment Approaches of Plant Genetic Diversity

Bhanu,

Bhandari,

Shukla

et al. 2024

Sustainable Development and Biodiversity

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Genetic Diversity and Population Structure Analysis in Indian Mustard Germplasm Using Phenotypic Traits and SSR Markers

Cited by 10 publications

References 37 publications

Genetic diversity and population structure of sweet potato landraces based on phenotypic traits, carotenoid content, and SSR molecular markers

Genetic diversity and population structure of sweet potato landraces based on phenotypic traits, carotenoid content, and SSR molecular markers

Development of non‐destructive models to predict oil content and fatty acid composition of Gomenzer (Ethiopian mustard) using near‐infrared reflectance spectroscopy

Innovations in Assessment Approaches of Plant Genetic Diversity

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