Genetic diversity and pathogenicity of novel chicken astrovirus in China

Luan, Qingdong; Han, Yijun; Yin, Ye; Wang, Jianlin

doi:10.1080/03079457.2022.2102966

Cited by 2 publications

(10 citation statements)

References 37 publications

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“…CAstV strains could be transmitted horizontally within chicken flocks via the faecal-oral route, and some strains may be vertically transmitted, causing hatchery diseases ( McIlwaine et al, 2021 ; Raji et al, 2022 ). Recently, more and more clinical cases of CAstV infection are found in China ( Xue et al, 2017 ; Zhao et al, 2020 , 2021 ; Luan et al, 2022 ; Zhang et al, 2022 ). Nevertheless, there exists little information concerning the systematic epidemiology of CAstV infection.…”

Section: Discussionmentioning

confidence: 99%

“…CAstV strains, based on phylogenetic analysis and the genetic distance of the ORF2 amino acid sequences, can be divided into two distinct genogroups (A and B): Group A with three subgroups (Ai, Aii, and Aiii), and Group B consists of six subgroups (Bi, Bii, Biii, Biv, Bv, and Bvi) ( Smyth, 2017 ; Raji et al, 2022 ; Raji and Omar, 2022a , b ). The previous studies have reported that diverse CAstV strains (subgroup Ai, subgroup Bi, subgroup Bii) are detected circulating in Chinese chicken flocks ( Zhao et al, 2020 ; Luan et al, 2022 ). In this study, 31 CAstV isolates were characterized and analyzed, and sequence alignment based on the ORF2 amino acid showed that the 31 CAstV isolates shared 37.5–99.9% similarities with the 57 CAstV reference strains.…”

Section: Discussionmentioning

confidence: 99%

“…The two US strains (CkP5/US/2016 and CC_CkAstV/US/2014) belonging to subgroup Biv are shown to act as the aetiologic agent of the runting-stunting syndrome in broiler chickens ( Kang et al, 2018 ). The white chick hatchery disease, caused by CAstV subgroup Ai, Aiii, Bi, Bii, and Biv, has been found in Poland, China, Canada, and Brazil ( Smyth et al, 2013 ; Sajewicz-Krukowska et al, 2016 ; Palomino-Tapia et al, 2020 ; Luan et al, 2022 ). In accordance with the amino acid sequence of ORF2, the sequence alignments showed that JS202103 isolate had a high similarity with the ANAND-IN-2016 strain (subgroup Biii), but a phylogenetic analysis indicated that JS202103 isolate belonged to a new B subgroup.…”

Section: Discussionmentioning

confidence: 99%

“…Since 2016, CAstV infections have been detected circulating in China ( Xue et al, 2017 ; Zhao et al, 2020 ; Yin et al, 2021 ; Luan et al, 2022 ). For example, Xue et al did first serological investigation of CAstV infection and showed that CAstV infection is very common in China ( Xue et al, 2017 ).…”

Section: Introductionmentioning

confidence: 99%

“…Yin et al, isolated a novel and recombinant CAstV strain (GD202013, subgroup Bii) leading to enteritis, swollen kidney, enlarged liver with numerous hemorrhages, and abscission in small intestines in SPF chicken embryos ( Yin et al, 2021 ). Luan et al, isolated three types of genotypic CAstV (subgroup Ai, Bi and Bii), resulting in high mortality in SPF chicken embryos and leading to WCS and visceral gout in SPF chickens ( Luan et al, 2022 ).…”

Section: Introductionmentioning

confidence: 99%

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Molecular characterization of chicken astrovirus and pathogenicity of a novel isolate in China

Han,

Yin,

Liang

et al. 2023

Front. Microbiol.

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As an enteric virus, chicken astrovirus has been related to various kinds of diseases in chickens, including white chick syndrome, runting-stunting syndrome, severe kidney disease, urate deposits and visceral gout, generating economic losses in the poultry industry globally. The complete ORF2 gene of 31 CAstV isolates in six provinces of China during 2020–2022 was characterized and analyzed with the purpose of better understanding the molecular epidemiology and genetic diversity of CAstV field isolates. Phylogenetic analysis which was based on the complete ORF2 (capsid) amino acid sequence of 31 CAstV isolates and 57 reference strains indicated that 2 isolates belonged to subgroup Ai, 10 isolates belonged to subgroup Bi, 3 isolates belonged to subgroup Bii, 5 isolates belonged to subgroup Biii, 7 isolates belonged to subgroup Biv, 3 isolates belonged to subgroup Bv, and one isolate (JS202103) belonged to a new B subgroup. In addition, the novel CAstV strain JS202103 was successfully isolated in vitro, and its whole genome shared 76.9–94.3% identity with the 29 CAstV reference strains. JS202103 caused hatchability reduction, dead embryos, kidney disease and visceral gout in chicken embryos. Moreover, this is the also the initial study focusing on diverse CAstV strains including subgroups Biii, Biv, and Bv circulate in China. The current work contributes to improving our understanding of CAstV isolates in China, and it will also provide references for developing efficient measures to control this virus.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Molecular characterization of chicken astrovirus and pathogenicity of a novel isolate in China

Han,

Yin,

Liang

et al. 2023

Front. Microbiol.

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Genome Sequences and Characterization of Chicken Astrovirus and Avian Nephritis Virus from Tanzanian Live Bird Markets

Kariithi

Volkening

Chiwanga

et al. 2023

Viruses

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The enteric chicken astrovirus (CAstV) and avian nephritis virus (ANV) are the type species of the genus Avastrovirus (AAstV; Astroviridae family), capable of causing considerable production losses in poultry. Using next-generation sequencing of a cloacal swab from a backyard chicken in Tanzania, we assembled genome sequences of ANV and CAstV (6918 nt and 7318 nt in length, respectively, excluding poly(A) tails, which have a typical AAstV genome architecture (5′-UTR-ORF1a-ORF1b-ORF2-‘3-UTR). They are most similar to strains ck/ANV/BR/RS/6R/15 (82.72%) and ck/CAstV/PL/G059/14 (82.23%), respectively. Phylogenetic and sequence analyses of the genomes and the three open reading frames (ORFs) grouped the Tanzanian ANV and CAstV strains with Eurasian ANV-5 and CAstV-Aii viruses, respectively. Compared to other AAstVs, the Tanzanian strains have numerous amino acid variations (substitutions, insertions and deletions) in the spike region of the capsid protein. Furthermore, CAstV-A has a 4018 nt recombinant fragment in the ORF1a/1b genomic region, predicted to be from Eurasian CAstV-Bi and Bvi parental strains. These data should inform future epidemiological studies and options for AAstV diagnostics and vaccines.

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Genetic diversity and pathogenicity of novel chicken astrovirus in China

Cited by 2 publications

References 37 publications

Molecular characterization of chicken astrovirus and pathogenicity of a novel isolate in China

Molecular characterization of chicken astrovirus and pathogenicity of a novel isolate in China

Genome Sequences and Characterization of Chicken Astrovirus and Avian Nephritis Virus from Tanzanian Live Bird Markets

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