Genetic diversity and Multilocus Sequence Typing Analysis of Bartonella henselae in domestic cats from Southeastern Brazil

Furquim, Maria Eduarda Chiaradia; Amaral, Renan do; Dias, Clara Morato; Gonçalves, Luiz Ricardo; Perles, Lívia; Lima, Cirilo Antonio de Paula; Barros-Battesti, Darci Moraes; Machado, Rosangela Zacarias; André, Marcos Rogério

doi:10.1016/j.actatropica.2021.106037

Cited by 22 publications

(10 citation statements)

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“…Unfortunately, information about outdoor access and exposure to ticks were not available, precluding the assessment of associated risk factors for positivity. Some cats sampled in SP and MG were parasitized by Ctenocephalides felis felis fleas at the time of blood sampling (data not shown) [32]. It is most likely that positive cats might have presented long-lasting erythroparasitemia and may act as reservoirs for non-pathogenic strains of Cytauxzoon in Brazil, which should be confirmed by experimental infection of cats with Brazilian strains of Cytauxzoon.…”

Section: Discussionmentioning

confidence: 91%

“…Between the months of August and September 2018, 390 blood samples were collected from domiciled cats in Minas Gerais (155 samples-Uberlândia 19°00′39" S 48°05′45″ W and Araguari 18°24′43" S 49°03′09″ W), São Paulo (151 samples-Jaboticabal 21°15′33″ S 48°18′54″ W) and Rondônia (84 samples-Rolim de Moura 11°43′21.8″ S 61°46′29.5″ W) states (Figure 4). These samples were collected with the consent of the animals' tutors during neutering campaigns promoted by the Animal Protector Association Neutering Center, located at the School of Agricultural and Veterinarian Sciences (FCAV/ UNESP, Jaboticabal, São Paulo State, Southeastern Brazil), Animal Populational Control Project a partnership by the Federal University of Uberlândia (UFU) and the city's Zoonosis Control Center (Uberlândia, Minas Gerais State) [32] and by researchers of Universidade Federal de Rondônia. All procedures were authorized and approved by the Animal Use Ethics Committee of the Universidade Estadual Paulista (IACUC FCAV/UNESP 012017/17).…”

Section: Cats' Blood Samplingmentioning

confidence: 99%

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Molecular Detection of Tick-Borne Agents in Cats from Southeastern and Northern Brazil

et al. 2022

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Even though the epidemiology of tick-borne agents (TBA) in dogs has been extensively investigated around the world, the occurrence, vectors involved, and molecular identity of these agents in cats remains elusive in many regions. Among TBA, Ehrlichia, Anaplasma, Babesia, Cytauxzoon, and Hepatozoon are responsible for diseases with non-specific clinical signs in cats, making essential the use of molecular techniques for accurate diagnosis and proper treatment. The present work aimed to investigate the occurrence and molecular identity of tick-borne agents (Ehrlichia, Anaplasma, Babesia/Theileria, Cytauxzoon, and Hepatozoon) in cats from southeastern (states of São Paulo (SP) and Minas Gerais (MG)) and northern (state of Rondônia (RO)) Brazil. For this purpose, 390 blood samples were collected from domiciled cats in MG (n = 155), SP (n = 151), and RO(n = 84) states, submitted to DNA extraction and PCR assays for Ehrlichia spp. (dsb gene), Anaplasma spp. (rrs gene), piroplasmids (18S rRNA gene), and Hepatozoon spp. (18S rRNA gene), sequencing, and phylogenetic inferences. The overall positivity for Anaplasma spp., Ehrlichia spp., Babesia/Theileria spp., Cytauxzoon spp., and Hepatozoon spp. were 7.4% (12.3% (MG) and 6.6% (SP)), 2% (4.5% (MG) and 0.6% (SP)), 0.7% (0.6% (MG), 0.6% (SP) and 1.2% (RO)), 27.2% (41.9% (MG), 24.5% (SP) and 4.8% (RO), and 0%, respectively. The phylogenetic analysis grouped the obtained sequences with ‘Candidatus Anaplasma amazonensis’, A. platys, B. vogeli, and Cytauxzoon sp. previously detected in wild felids from Brazil. qPCR specific for E. canis based on the dsb gene confirmed the molecular identity of the detected ehrlichial agent. The present study expanded the list and geographical distribution of hemoparasites in cats. ‘Candidatus Anaplasma amazonensis’, recently detected in sloths from northern Brazil, was described for the first time in cats. This is the first report of piroplasmids infecting cats in northern Brazil. Coinfection by Cytauxzoon and other TBA (Ehrlichia, Anaplasma, and B. vogeli) reported in the present study raises the need for veterinary practitioners’ awareness of cats parasitized by multiple TBA.

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Section: Discussionmentioning

confidence: 91%

Section: Cats' Blood Samplingmentioning

confidence: 99%

Molecular Detection of Tick-Borne Agents in Cats from Southeastern and Northern Brazil

et al. 2022

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“…Flea infestation status is particularly important, considering that all the cats in this study had ectoparasites on their body surface. The prevalence of Bartonella DNA detected in C. felis fleas varies worldwide, ranging from 7.3% to 75.6% (Rolain et al, 2003;Alves et al, 2009;Tsai et al, 2011;Gutiérrez et al, 2015;Rizzo et al, 2015;Furquim et al, 2021). Bartonella DNA was not detected in R. sanguineus (s.l.)…”

Section: Discussionmentioning

confidence: 97%

Prevalence of Bartonella species in shelter cats and their ectoparasites in southeastern Brazil

Raimundo

Guimarães

Amaro

et al. 2022

Rev. Bras. Parasitol. Vet.

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Feline Bartonella can be transmitted to humans through cat scratches or bites, and between cats, by the flea Ctenocephalides felis. The study was carried out in order to investigate the occurrence of Bartonella DNA in cats living in shelters and their ectoparasites and the relationship between the infection status of cats and ectoparasites they host. Bartonella DNA was detected in 47.8% of the cat blood samples, 18.3% of C. felis fleas, 13.3% of flea egg pools and 12.5% of lice pools. B. henselae and B. clarridgeiae DNA were detected in cat fleas, while B. henselae, B. clarridgeiae and B. koehlerae were found in blood samples from bacteremic cats. Cats infested by positive ectoparasites showed approximately twice the odds of being infected. Our results indicate that shelter cats have high prevalence of Bartonella species that are known to be human pathogens. This highlights the importance of controlling infestations by ectoparasites to avoid cat and human infection.

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“…As a result, younger cats are more prone to present peaks of parasitaemia, which ultimately can facilitate pathogen detection [ 19 , 20 ]. It is important to denote that the combination of Bartonella alphaproteobacteria growth medium (BAPGM) followed by qPCR and chocolate agar culture plus the new qPCR round was not employed in the cat blood samples screened which would increase sensitivity and possibly yield in a higher number of positive samples [ 21 ].…”

Section: Discussionmentioning

confidence: 99%

“…Multilocus sequence typing (MLST) studies have been conducted on Bartonella spp. contributing to clarifying phylogenetic relationships, host specificity, diversity and the discovery of novel species [ 21 , 27 , 28 , 29 ].…”

Section: Discussionmentioning

confidence: 99%

Molecular Survey of Bartonella Species in Stray Cats and Dogs, Humans, and Questing Ticks from Portugal

et al. 2022

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Bartonella spp. comprises emergent and re-emergent fastidious Gram-negative bacteria with worldwide distribution. Cats are the main reservoir hosts for Bartonella henselae and dogs represent opportunistic hosts for the bacteria. Even though ticks may also play a role in transmission, their competence as vectors for Bartonella spp. has not been totally understood. Considering only a few studies had a focus on screening Bartonella in animals, humans and ectoparasites in Portugal, this study aimed to address the molecular occurrence of Bartonella sp. in 123 stray cats, 25 stray dogs, 30 humans from Lisbon and 236 questing ticks within the country. Using a qPCR targeting the nuoG gene, it was possible to detect Bartonella sp. DNA on 20.32% of cat samples (25/123). From these positive samples, 13 sequences were characterized as B. henselae, 11 as B. clarridgeiae and 1 presented co-infection with both species. The absolute quantification of nuoGBartonella DNA in sampled cats ranged from 2.78 × 10 to 1.03 × 105 copies/µL. The sampled dogs, humans and ticks were negative. These results showed that B. henselae and B. clarridgeiae are circulating in stray cats from Lisbon. Additional and more extended studies should be conducted to determine the impact of such infections on humans, particularly those in constant and direct contact with cats.

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Genetic diversity and Multilocus Sequence Typing Analysis of Bartonella henselae in domestic cats from Southeastern Brazil

Cited by 22 publications

References 59 publications

Molecular Detection of Tick-Borne Agents in Cats from Southeastern and Northern Brazil

Molecular Detection of Tick-Borne Agents in Cats from Southeastern and Northern Brazil

Prevalence of Bartonella species in shelter cats and their ectoparasites in southeastern Brazil

Molecular Survey of Bartonella Species in Stray Cats and Dogs, Humans, and Questing Ticks from Portugal

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