Genetic Diversity and Molecular Epidemiology of Mycobacterium tuberculosis in Roraima State, Brazil

Tatara, Mariana Bento; Perdigão, João; Viveiros, Miguel; Kritski, Afrânio Lineu; Silva, Késia Esther da; Sacchi, Flávia Patussi Correia; Lima, Camila Camioli de; Santos, Paulo César Pereira dos; Diniz, Jaciara; Silva, Pedro Eduardo Almeida; Gomes, Pedro; Gomes, Mônica Maria Quirino; Cunha, Eunice Atsuko Totumi; Silva, José Roberto Lapa e; Portugal, Isabel; Croda, Júlio; Andrade, Mônica Kramer de Noronha

doi:10.4269/ajtmh.19-0324

Cited by 4 publications

(5 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The potential of WGS to provide a quick and comprehensive view of the genotype and reliable prediction of the lineages has been extensively reported [ 5 , 7 , 14 , 40 ]; thus, its application in surveillance programs is necessary. The Euro-American lineage (lineage 4) presents a variable distribution in Argentina with a predominance of M and Ra strains [ 41 ], for Brazil, lineage 4 varies between 25–99% [ 13 , 42 , 43 ], for Colombia, between 85–99% [ 44 – 46 ], for Mexico between 69–98% [ 47 , 48 ] and for Peru around 90% [ 12 , 49 , 50 ]. Ecuador is not an exception, in fact, all the samples analyzed by us belong to the Euro-American lineage.…”

Section: Discussionmentioning

confidence: 99%

“…This lineage has been described as more transmissible than others [10]. From 10 sub-lineages, including in Lineage 4, LAM (L4.3), Haarlem (L4.1.2), X-type and T families are the most observed members of the Euro-American lineage in South and Central America and the Caribbean, a distribution profile shared with Europe and Middle Africa [11][12][13][14].…”

Section: Introductionmentioning

confidence: 96%

See 1 more Smart Citation

Comparative genomics of drug-resistant strains of Mycobacterium tuberculosis in Ecuador

et al. 2022

View full text Add to dashboard Cite

Background Tuberculosis is a serious infectious disease affecting millions of people. In spite of efforts to reduce the disease, increasing antibiotic resistance has contributed to persist in the top 10 causes of death worldwide. In fact, the increased cases of multi (MDR) and extreme drug resistance (XDR) worldwide remains the main challenge for tuberculosis control. Whole genome sequencing is a powerful tool for predicting drug resistance-related variants, studying lineages, tracking transmission, and defining outbreaks. This study presents the identification and characterization of resistant clinical isolates of Mycobacterium tuberculosis including a phylogenetic and molecular resistance profile study by sequencing the complete genome of 24 strains from different provinces of Ecuador. Results Genomic sequencing was used to identify the variants causing resistance. A total of 15/21 isolates were identified as MDR, 4/21 as pre-XDR and 2/21 as XDR, with three isolates discarded due to low quality; the main sub-lineage was LAM (61.9%) and Haarlem (19%) but clades X, T and S were identified. Of the six pre-XDR and XDR strains, it is noteworthy that five come from females; four come from the LAM sub-lineage and two correspond to the X-class sub-lineage. A core genome of 3,750 genes, distributed in 295 subsystems, was determined. Among these, 64 proteins related to virulence and implicated in the pathogenicity of M. tuberculosis and 66 possible pharmacological targets stand out. Most variants result in nonsynonymous amino acid changes and the most frequent genotypes were identified as conferring resistance to rifampicin, isoniazid, ethambutol, para-aminosalicylic acid and streptomycin. However, an increase in the resistance to fluoroquinolones was detected. Conclusion This work shows for the first time the variability of circulating resistant strains between men and women in Ecuador, highlighting the usefulness of genomic sequencing for the identification of emerging resistance. In this regard, we found an increase in fluoroquinolone resistance. Further sampling effort is needed to determine the total variability and associations with the metadata obtained to generate better health policies.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 96%

Comparative genomics of drug-resistant strains of Mycobacterium tuberculosis in Ecuador

et al. 2022

View full text Add to dashboard Cite

show abstract

“…When analyzing the population included in this study, it is observed that the mean age of the population coincides with that found in the state of Veracruz, Mexico, and in the state of Romaira, Brazil. [31, 32] It differs from that reported in the foreign population in Finland where the mean age is 28 years and in Tokyo, Japan, where an age of less than 40 years is reported. [22, 33] It is evident that the mean age of the patients is lower as the incidence of the disease increases.…”

Section: Discussionmentioning

confidence: 73%

Genetic patterns, sublineages and dynamics of tuberculosis transmission in Cuba, a low burden country

Martinez-Rodriguez,

Herrera-Avila,

Goza-Valdes

et al. 2024

Preprint

View full text Add to dashboard Cite

Context: In Cuba, a country with a low incidence of tuberculosis (TB), there is no information on the dynamics of transmission of the disease for two decades. Aims: Determine the genetic patterns and sublineages of Mycobacterium tuberculosis complex (MTBC) isolates circulating in 2009 and their relationship with the epidemiological data of the patients. Settings and Design: A retrospective descriptive study was carried out in Cuba with 178 MTBC isolates. Methods and Material: Spoligotyping and mycobacterial interspersed repetitive unit variable number tandem repeat (MIRU-VNTR) typing with 24 loci were performed. Statistical analysis used: Nonparametric methods, analysis of variance and homogeneity test, using the statistical package Statgraphics Plus version 2.1 were used. Results: Spoligotyping produced 39 spoligotypes. The S, Beijing, LAM and Haarlem sublineages prevailed. The clustering rate was 75.84% and the Hunter-Gaston discriminatory index (HGDI): 0.8734. MIRU-VNTR typing with 24 loci defined 154 genetic patterns: six grouped 30 isolates and 148 showed unique patterns. The clustering rate was 14.60% and the HGDI: 0.9926. There was a predominance of sublineages by region: S, Beijing and LAM in the West, Center and East, respectively. Conclusions: The dynamics of TB transmission in Cuba in 2009 is reported, inferring that this occurs in a limited manner and belonging to risk groups does not favor transmission. It serves to evaluate a megaproject of the Global Fund to reduce the transmission of TB in this country. The population genetic structure of MTBC resembles that of an Ibero-American country, with the exception of the high frequency of the Beijing and S sublineages.

show abstract

“…Similarly, the North region was also represented by a single study conducted in the State of Roraima which borders with Venezuela and Guyana and has an important portion of their TB cases associated with indigenous population, constituting 70% of TB cases (2015-2016) and presenting a clustering rate of 30%. 31 This clustering rate is low when compared to other regions of the country what might be related to a large flow of people, being a border region.…”

Section: Resultsmentioning

confidence: 93%

“…Fig. 2 demonstrates details about the screening process and finally: 17 manuscripts on MIRU-VNTR 17 23 - 39 and 42 on RFLP-IS 6110 were considered; 23 , 26 , 31 , 32 , 40 - 75 three manuscripts considered both methodologies.…”

Section: Resultsmentioning

confidence: 99%

Molecular epidemiology of Mycobacterium tuberculosis in Brazil before the whole genome sequencing era: a literature review

Conceição

Salvato

Gomes

et al. 2021

Mem. Inst. Oswaldo Cruz

View full text Add to dashboard Cite

Molecular-typing can help in unraveling epidemiological scenarios and improvement for disease control strategies. A literature review of Mycobacterium tuberculosis transmission in Brazil through genotyping on 56 studies published from 1996-2019 was performed. The clustering rate for mycobacterial interspersed repetitive units - variable tandem repeats (MIRU-VNTR) of 1,613 isolates were: 73%, 33% and 28% based on 12, 15 and 24-loci, respectively; while for RFLP-IS 6110 were: 84% among prison population in Rio de Janeiro, 69% among multidrug-resistant isolates in Rio Grande do Sul, and 56.2% in general population in São Paulo. These findings could improve tuberculosis (TB) surveillance and set up a solid basis to build a database of Mycobacterium genomes.

show abstract

Genetic Diversity and Molecular Epidemiology of Mycobacterium tuberculosis in Roraima State, Brazil

Cited by 4 publications

References 31 publications

Comparative genomics of drug-resistant strains of Mycobacterium tuberculosis in Ecuador

Comparative genomics of drug-resistant strains of Mycobacterium tuberculosis in Ecuador

Genetic patterns, sublineages and dynamics of tuberculosis transmission in Cuba, a low burden country

Molecular epidemiology of Mycobacterium tuberculosis in Brazil before the whole genome sequencing era: a literature review

Contact Info

Product

Resources

About