Genetic diversity and molecular diagnosis of Giardia

Chang, Yankai; Li, Junqiang; Zhang, Longxian

doi:10.1016/j.meegid.2023.105482

Cited by 6 publications

(3 citation statements)

References 154 publications

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“…The age range of the patients in the study varied from 2 to 46 years old. Notably, 71% of the patients were over the age of 15, which aligns with similar findings from previous studies ( 9 , 10 ). Additionally, data collected from one study indicate a higher prevalence of positive cases among male patients.…”

Section: Discussionsupporting

confidence: 92%

“…Amplification was carried out by semi-nested PCR method using the specific primer pair of gdh gene in two rounds; the first round with 5’- GACGCATCAACGTCAACC-3’ and 5’- GAGCTTCTCGCAAGCAAAC-3’ at 58 °C for annealing step and the second round with 5’-CAGTACAACTCTGCTCTCGG-3’5’-GTTGTCCTTGCACATCTCC-3’ at 61 °C for annealing the primers. The amplicon sizes were 539 and 432, respectively ( 10 ). Amplification in both rounds was done using Taq DNA Polymerase Master Mix Red (2X; Amplicon, Denmark) and 0.5 mM of each primer.…”

Section: Methodsmentioning

confidence: 99%

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Molecular Genotyping of Giardia duodenalis in Humans in the Yazd County, Central of Iran

Hezardastan,

Bafghi,

Eslami

et al. 2024

IJPA

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Background: We aimed to investigate the molecular genotyping of Giardia duodenalis in Humans in the Yazd County, Central, Iran. Methods: Total of 35 fecal samples were collected from patients referred to Yazd Central Laboratory, Yazd, Iran from February to July 2022. All the samples were included in this study after microscopic observation of G. duodenalis. DNA samples were extracted using related kit and were analyzed by Nano Drop. The molecular assessment was carried out using semi-nested PCR using the target gene of gdh. All amplified samples were sequenced using Sanger method. BLAST analyzed the sequences for assemblage identification. Results: Out of 35 samples, 24 (68.57%) and 11 (31.43%) were male and female, respectively. All included samples were amplified using the specific gdh primer pair. The molecular analysis showed 17 isolates (48.57%) as assemblage BIV, 8 isolates (22.86%) as assemblage BIII, 6 isolates (17.14%) as assemblage AII and 4 isolates (11.43%) as assemblage AIII (P<0.05). Conclusion: Assemblages A and B are the most prevalent in Central Iran. The molecular identification of G. duodenalis isolates from animals and implementing control programs

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Section: Discussionsupporting

confidence: 92%

Section: Methodsmentioning

confidence: 99%

Molecular Genotyping of Giardia duodenalis in Humans in the Yazd County, Central of Iran

Hezardastan,

Bafghi,

Eslami

et al. 2024

IJPA

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show abstract

“…The use of molecular techniques such as PCR and further analysis of the amplicon by restriction fragment length polymorphism (RFLP), i.e., PCR-RFLP analysis, have provided a clear distinction between different assemblages (genotypes) of G. intestinalis isolates. [ 17 ] In another Indian study, stool microscopy detected 65%, stool PCR detected an additional 27%, and duodenal biopsy PCR detected an additional 8% of giardiasis cases in chronically ill patients. Stool-nested PCR had a sensitivity and specificity of 100% and 94%, respectively, compared to stool microscopy.…”

Section: Discussionmentioning

confidence: 99%

Molecular appraisal of Giardia intestinalis from Western India: A prospective observational study

Mohammad,

Tak,

Bohra

et al. 2024

Tropical Parasitology

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Background: Giardia intestinalis is an intestinal protozoan which commonly causes parasitic gastroenteritis globally. It is a species complex consisting of at least eight assemblages (genotypes). In India, Giardia is mostly underreported and missed in asymptomatic cases. Aim: The aim of this study was to genotype the G. intestinalis isolates from stool samples of patients at a tertiary care center in Rajasthan, India, and to clinically correlate it. Methods: This prospective pilot cross-sectional study was conducted from 2019 to 2021 in a tertiary care center in western India. Patients who were microscopically positive for giardiasis were enrolled. DNA was extracted from their stool samples and amplified by polymerase chain reaction (PCR) using 4E1-HP as the target sequence. Anthropometric measurements and analysis were done for children by using Anthrocal application. Results: A total of 50 patients were enrolled. Diarrhea was present in 18 patients (36%). Among these, 6 were immunocompromised and had different comorbidities. Among the children <12 years of age, 55.17% (n = 16/29) were stunted (<−2 S.D.), and among <5 years, 44.4% (n = 4/9) showed wasting (<−2 S.D.). A PCR product corresponding to assemblage B of G. intestinalis was amplified in 47 stool specimens. Only three stool samples were negative for both assemblages A and B and posed an interesting enigma. Conclusion: In this study, a predominance of assemblage B of G. intestinalis was detected in 94% of the isolates. Furthermore, the possibility of zoonotic transmission could not be ruled out.

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Assemblage characterization of Giardia duodenalis in South Khorasan province, eastern Iran, using HRM real-time PCR method

Tabrizi,

Behravan,

Seyyed Tabaei

et al. 2024

Mol Biol Rep

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Genetic diversity and molecular diagnosis of Giardia

Cited by 6 publications

References 154 publications

Molecular Genotyping of Giardia duodenalis in Humans in the Yazd County, Central of Iran

Molecular Genotyping of Giardia duodenalis in Humans in the Yazd County, Central of Iran

Molecular appraisal of Giardia intestinalis from Western India: A prospective observational study

Assemblage characterization of Giardia duodenalis in South Khorasan province, eastern Iran, using HRM real-time PCR method

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