Genetic diversity and methicillin resistance of Staphylococcus aureus originating from buffaloes with mastitis in Iran

Panahi, Mitra; Saei, Habib Dastmalchi

doi:10.1016/j.cimid.2018.11.014

Cited by 10 publications

(5 citation statements)

References 43 publications

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“…t304/ST8 has previously been described in Caribbean countries and Norwegian nursing homes outbreaks from 1998 to 2010 [25,26] and a study from Iran reported t304/ST291 in mastitis isolates from buffalos [27]. Based on WGS data from 2011 to June 2019 at Hvidovre Hospital less than 2% of our t304 isolates belong to STs other than ST6 (six ST1649, three ST8 and one ST4290).…”

Section: Discussionmentioning

confidence: 55%

Repeated introduction and spread of the MRSA clone t304/ST6 in northern Europe

Bartels

Worning

Andersen

et al. 2021

Clinical Microbiology and Infection

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Objectives: During the last decades several methicillin-resistant Staphylococcus aureus (MRSA) clones with the capability of global spread have emerged in the community. Here, we have investigated a large collection of clinical isolates belonging to MRSA clone t304/ST6, which has emerged in many European countries over the last years, in order to retrace its phylogeny and its spread. Methods: We characterized 466 ST6 isolates from Denmark (n ¼ 354), France (n ¼ 10), Norway (n ¼ 24), Sweden (n ¼ 27) and the UK (n ¼ 51). All had spa-type t304 (n ¼ 454) or t304-related spa-types (n ¼ 12) and whole genome sequencing (WGS) was carried out on Illumina Miseq or Hiseq with 100e300 bp reads. cgMLST was performed using Ridom SeqSphere. Results: A minimum spanning tree (MST) of all 466 isolates showed one large cluster including 182 isolates collected only from Denmark and related to a long-term neonatal outbreak in Copenhagen. This cluster contrasted with numerous small clusters, including the remaining Danish isolates and isolates from the other countries that interspersed throughout the tree. Most isolates were Panton-Valentine leukocidin (PVL) negative (95%) and harboured SCCmec IVa. One genome was closed using Oxford Nanopore technology and Illumina MiSeq. It contained a plasmid of 19.769 bp including the blaZ gene. A similar plasmid was found in 78% of all isolates. Discussion: t304/ST6 is a successful emerging clone and the fact that isolates from five countries are interspersed throughout the MST indicates a common origin. This clone is commonly described in the Middle East and its emergence in Europe coincides with influx of refugees from the Syrian Civil War.

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Section: Discussionmentioning

confidence: 55%

Repeated introduction and spread of the MRSA clone t304/ST6 in northern Europe

Bartels

Worning

Andersen

et al. 2021

Clinical Microbiology and Infection

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“…The ST398 strains belong to the human-associated lineage of ST398 60 and they evolved via mutations in grlA . The ST291 strains are genetically quite different from ST398 and represent a separate lineage within CC398 61 ; they are usually associated with humans 62 , but more recently isolated from livestock 63 , 64 . These ST291 strains (and the related ST3535 strain) rapidly and repeatedly evolved in response to ciprofloxacin by ISSau1-mediated tandem amplification of norA 34 , allowing them to increase ciprofloxacin resistance without any detectable fitness cost.…”

Section: Discussionmentioning

confidence: 99%

Efflux pump activity potentiates the evolution of antibiotic resistance across S. aureus isolates

et al. 2020

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The rise of antibiotic resistance in many bacterial pathogens has been driven by the spread of a few successful strains, suggesting that some bacteria are genetically pre-disposed to evolving resistance. Here, we test this hypothesis by challenging a diverse set of 222 isolates of Staphylococcus aureus with the antibiotic ciprofloxacin in a large-scale evolution experiment. We find that a single efflux pump, norA, causes widespread variation in evolvability across isolates. Elevated norA expression potentiates evolution by increasing the fitness benefit provided by DNA topoisomerase mutations under ciprofloxacin treatment. Amplification of norA provides a further mechanism of rapid evolution in isolates from the CC398 lineage. Crucially, chemical inhibition of NorA effectively prevents the evolution of resistance in all isolates. Our study shows that pre-existing genetic diversity plays a key role in shaping resistance evolution, and it may be possible to predict which strains are likely to evolve resistance and to optimize inhibitor use to prevent this outcome.

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“…The ST97-PVL + MSSA has been reported only in one human case from Bali [26]. The ST398 is frequently isolated from pigs [1,2,27] and the ST97 is globally linked with bovine mastitis [2,28,29]. In addition, all these types of MSSA have been reported from a variety of clinical infections [30,31,32,33,34].…”

Section: Discussionmentioning

confidence: 99%

Distribution of Toxinogenic Methicillin-Resistant and Methicillin-Susceptible Staphylococcus aureus from Different Ecological Niches in Algeria

Mairi

Touati

Pantel

et al. 2019

Toxins

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The diffusion of Panton–Valentine leukocidin (PVL)–positive methicillin-resistant S. aureus (MRSA) is a health problem in Algeria. The objectives of the study were to investigate the global distribution of methicillin-susceptible S. aureus (MSSA) and MRSA isolates in different ecological niches in this country. In total, 2246 samples were collected from humans, livestock, wild animals, pets, food products and the aquatic environment, from 12 Algerian provinces. A total of 312 S. aureus were detected from 2446 samples (12.7%) in the screened niches. We observed the emergence of toxinogenic S. aureus representing 41% of the isolates. Among them, we noted the diffusion of ST80-IV CA-MRSA PVL + strains isolated in human, animals, and food and genetic diversity of MSSA PVL + isolates. This study suggests an alarming dissemination of MRSA-ST80 PVL + in both human and extra-human sources in Algeria. Moreover, MSSA may become a permanent reservoir of the PVL genes necessary for human infections.

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Genetic diversity and methicillin resistance of Staphylococcus aureus originating from buffaloes with mastitis in Iran

Cited by 10 publications

References 43 publications

Repeated introduction and spread of the MRSA clone t304/ST6 in northern Europe

Repeated introduction and spread of the MRSA clone t304/ST6 in northern Europe

Efflux pump activity potentiates the evolution of antibiotic resistance across S. aureus isolates

Distribution of Toxinogenic Methicillin-Resistant and Methicillin-Susceptible Staphylococcus aureus from Different Ecological Niches in Algeria

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