Genetic diversity and exoenzyme activities of Candida albicans and Candida dubliniensis isolated from the oral cavity of Brazilian periodontal patients

Barros, Letízia Monteiro de; Boriollo, Marcelo Fabiano Gomes; Alves, Ana Cláudia Braga Amoras; Klein, Marlise I.; Gonçalves, Reginaldo Bruno; Höfling, José Francisco

doi:10.1016/j.archoralbio.2008.06.003

Cited by 55 publications

(50 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…albicans was the most commonly identified Candida species in this group of patients with periodontitis, which is in agreement with data from several previous studies (1,5,8,10,32,35). This species was most commonly recovered from 10/21 (47.6%) periodontitis patients both by oral rinse sampling and by the sampling of periodontal pockets using both paper points and curettes.…”

Section: Discussionsupporting

confidence: 79%

“…To our knowledge, this is the first study to examine C. albicans and C. dubliniensis isolates recovered from periodontal pockets by MLST, which is now considered the gold standard for molecular typing and population analysis of Candida species. Previous studies of subgingival Candida isolates relied on less-reproducible, subjective techniques such as RAPD and electrophoretic karyotyping (1,24,33). The advantage of MLST is that it relies on species-specific DNA-sequence-based comparisons of isolates, and the data can be stored electronically in databases.…”

Section: Discussionmentioning

confidence: 99%

“…Several previous studies investigated the genetic relatedness of C. albicans isolates recovered from the periodontal pockets, gingival sulci, and oral mucosae of patients with periodontitis by using molecular typing techniques such as random amplified polymorphic DNA (RAPD) fingerprinting, electrophoretic karyotyping, and ABC genotyping, with the latter technique being based on the presence or absence of an intron in the 25S ribosomal DNA (rDNA) (1,24,32,33). Pizzo et al (24) previously used electrophoretic karyotyping to show that some C. albicans genotypes are unique to subgingival isolates, suggesting some adaptation to this environment.…”

mentioning

confidence: 99%

See 2 more Smart Citations

Enrichment of Multilocus Sequence Typing Clade 1 with Oral Candida albicans Isolates in Patients with Untreated Periodontitis

et al. 2012

View full text Add to dashboard Cite

cThis study investigated the prevalence and cell density of Candida species in periodontal pockets, healthy subgingival sites, and oral rinse samples of patients with untreated periodontitis. Twenty-one periodontitis patients underwent sampling at two periodontitis sites, and 19/21 of these patients underwent sampling at one periodontally healthy site. Both paper point and curette sampling techniques were employed. The periodontitis patients and 50 healthy subjects were also sampled by oral rinse. Candida isolates were recovered on CHROMagar Candida medium, and representative isolates were identified. Candida spp. were recovered from 10/21 (46.7%) periodontitis patients and from 16/50 (32%) healthy subjects. C. albicans predominated in both groups and was recovered from all Candida-positive subjects. Candida-positive periodontitis patients yielded Candida from periodontal pockets with average densities of 3,528 and 3,910 CFU/sample from curette and paper point samples, respectively, and 1,536 CFU/ml from oral rinse samples. The majority (18/19) of the healthy sites sampled from periodontitis patients were Candida negative. The 16 Candida-positive healthy subjects yielded an average of 279 CFU/ml from oral rinse samples. C. albicans isolates were investigated by multilocus sequence typing (MLST) to determine if specific clonal groups were associated with periodontitis. MLST analysis of 31 C. albicans isolates from periodontitis patients yielded 19 sequence types (STs), 13 of which were novel. Eleven STs belonged to MLST clade 1. In contrast, 16 C. albicans isolates from separate healthy subjects belonged to 16 STs, with 4 isolates belonging to clade 1. The distributions of STs between both groups were significantly different (P ‫؍‬ 0.04) and indicated an enrichment of C. albicans isolates in periodontal pockets, which warrants a larger study.

show abstract

Section: Discussionsupporting

confidence: 79%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

See 1 more Smart Citation

Enrichment of Multilocus Sequence Typing Clade 1 with Oral Candida albicans Isolates in Patients with Untreated Periodontitis

et al. 2012

View full text Add to dashboard Cite

show abstract

“…No association was observed between exoenzymatic activities and strains (ETs), strain clusters/highly related isolates, or even, subject characteristics (diabetic, non‐diabetic, and gender). The absence of association between a particular genotype or cluster of strains of C. albicans and the type, disease progression, and degree of control of diabetes mellitus (28), the production of exoenzymes, and the oral cavity sites (periodontal pocket, gingival sulcus, and oral mucosa) has been observed (5). In addition, a few studies found that enzymatic secretions varied for each isolate from different strains and origins (30, 63).…”

Section: Discussionmentioning

confidence: 99%

Distribution and hydrolytic enzyme characteristics of Candida albicans strains isolated from diabetic patients and their non‐diabetic consorts

Boriollo

Bassi

Nascimento³

et al. 2009

Oral Microbiology and Immunology

View full text Add to dashboard Cite

Possible sources of transmission and oral propagation of groups (clusters) of strains of C. albicans can occur between diabetic and non-diabetic consorts. A conjugal genotypic identity exists in most C. albicans-positive couples, that is, both consorts share identical or highly related strains; however, this identity is not couple-specific as seen by the coexistence of clusters in couples and unrelated consorts.

show abstract

“…[30] all C. albicans isolates were tested in duplicate for verification of the enzymatic activity of Saps and phospholipases. The test medium for proteinases was BSA agar medium containing 2 g of BSA, 1.45 g of YNB (Difco Laboratories, Detroit, MI, USA), 20 g of glucose and 20 g of agar per liter of distilled water.…”

Section: Methodsmentioning

confidence: 99%

Role of Candida albicans-Secreted Aspartyl Proteinases (Saps) in Severe Early Childhood Caries

Gao

et al. 2014

IJMS

View full text Add to dashboard Cite

Candida albicans is strongly associated with severe early childhood caries (S-ECC). However, the roles of secreted aspartyl proteinases (Saps), an important virulence factor of C. albicans, in the progress of S-ECC are not clear. In our study, the Saps activities were evaluated by the yeast nitrogen base–bovine serum albumi (YNB–BSA) agar plate method and by the MTT method with bovine serum albumin (BSA) as the substrate. Genotypes of C. albicans and gene expression of Sap1–5 were evaluated. The relationships of Saps activities and genotypes with S-ECC were analyzed. The results showed that enzyme activities of Saps in the S-ECC group were significantly higher than those in the caries free (CF) group (p < 0.05). Genotypes A, B and C were detected in the S-ECC group, and genotypes A and C were detected in the CF group. In the genotype A group, Saps activity in the S-ECC group was significantly different from that in the CF group (p < 0.05). The gene expression level of Sap1 in the S-ECC group was significantly higher than that in the CF group (p = 0.001), while Sap4 expression was significantly lower than that in the CF group (p = 0.029). It can be concluded that Sap1–5 are the predominant proteinase genes expressed in C. albicans from dental biofilm and Sap1 may play an important role in the development of S-ECC.

show abstract

Genetic diversity and exoenzyme activities of Candida albicans and Candida dubliniensis isolated from the oral cavity of Brazilian periodontal patients

Cited by 55 publications

References 40 publications

Enrichment of Multilocus Sequence Typing Clade 1 with Oral Candida albicans Isolates in Patients with Untreated Periodontitis

Enrichment of Multilocus Sequence Typing Clade 1 with Oral Candida albicans Isolates in Patients with Untreated Periodontitis

Distribution and hydrolytic enzyme characteristics of Candida albicans strains isolated from diabetic patients and their non‐diabetic consorts

Role of Candida albicans-Secreted Aspartyl Proteinases (Saps) in Severe Early Childhood Caries

Contact Info

Product

Resources

About