Genetic Diversity and Dynamic Distribution of Mycobacterium tuberculosis Isolates Causing Pulmonary and Extrapulmonary Tuberculosis in Thailand

Srilohasin, Prapaporn; Chaiprasert, Angkana; Tokunaga, Katsushi; Nishida, Nao; Prammananan, Therdsak; Smittipat, Nat; Mahasirimongkol, Surakameth; Chaiyasirinroje, Boonchai; Yanai, Hideki; Palittapongarnpim, Prasit

doi:10.1128/jcm.01467-14

Cited by 22 publications

(25 citation statements)

References 38 publications

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“…This study compared 163 isolates of M. tb from both pulmonary and extrapulmonary specimens and found the genetic diversity to be similar in both kinds of specimens indicating that these are similar stains circulating in the community and causing both forms of the disease. This differs from comparisons of M. tb isolates from both pulmonary and extrapulmonary samples in the literature, which found a higher genetic diversity in pulmonary tuberculosis patients [6].…”

Section: Discussioncontrasting

confidence: 99%

“…We recovered 163 isolates of M. tb from 86 pulmonary samples such as sputum (78), broncho-alveolar lavage (6), endobronchial ultrasound guided -trans bronchial needle aspiration (EBUS-TBNA) (2), and 77 extrapulmonary samples such as pus (21), lymph nodes (21), CSF (6), bone tissue (6), urine (5), endometrial curetting (3), pleural tissue (3), omental tissue (3), ascitic fluid (2), bone marrow (2), pleural fluid (2), lung tissue (1), synovial tissue (1) and mucosal tissue (1). These isolates were spoligotyped, resulting in 61 spoligo patterns.…”

Section: Resultsmentioning

confidence: 99%

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Spoligotyping of Mycobacterium tuberculosis isolates at a tertiary care hospital in India

Suzana

Shanmugam

Devi

et al. 2017

Tropical Med Int Health

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Section: Discussioncontrasting

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Spoligotyping of Mycobacterium tuberculosis isolates at a tertiary care hospital in India

Suzana

Shanmugam

Devi

et al. 2017

Tropical Med Int Health

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“…These discrepancies may be due to differences in the proportion of ancestral and modern Beijing strains. The percentage of ancestral Beijing strains is high in Japan (Iwamoto et al, 2009), whereas modern Beijing strains are more predominant in Thailand (Faksri et al, 2011;Srilohasin et al, 2014). This hypothesis is supported by Luo et al (2014), who found lower variability in VNTRs 2074 and 2372 from the ancestral Beijing strains than in the modern Beijing strains from China.…”

Section: Discussionmentioning

confidence: 96%

“…In general, 15-locus variable-number tandem repeats of mycobacterial interspersed repetitive units (MIRU-VNTR) and 24-locus MIRU-VNTR are standard methods used in routine epidemiologic investigations and phylogenetic analysis, respectively (Supply et al, 2006). However, these techniques have the disadvantage of low discriminatory power between the Beijing family of Mycobacterium tuberculosis strains (Allix-Béguec et al, 2014;Gurjav et al, 2014), which is the most prevalent genotype in Thailand (Faksri et al, 2011;Park et al, 2000;Prodinger et al, 2001;Srilohasin et al, 2014). Hypervariable loci have been proposed to improve the efficiency of subtyping Beijing clusters.…”

Section: Introductionmentioning

confidence: 99%

Genotypic diversity of multidrug-, quinolone- and extensively drug-resistant Mycobacterium tuberculosis isolates in Thailand

Disratthakit

Meada

Prammananan

et al. 2015

Infection, Genetics and Evolution

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Drug-resistant tuberculosis (TB), which includes multidrug-resistant (MDR-TB), quinolone-resistant (QR-TB) and extensively drug-resistant tuberculosis (XDR-TB), is a serious threat to TB control. We aimed to characterize the genotypic diversity of drug-resistant TB clinical isolates collected in Thailand to establish whether the emergence of drug-resistant TB is attributable to transmitted resistance or acquired resistance. We constructed the first molecular phylogeny of MDR-TB (n=95), QR-TB (n=69) and XDR-TB (n=28) in Thailand based on spoligotyping and proposed 24-locus multilocus variable-number of tandem repeat analysis (MLVA). Clustering analysis was performed using the unweighted pair group method with arithmetic mean. Spoligotyping identified the Beijing strain (SIT1) as the most predominant genotype (n=139; 72.4%). The discriminatory power of 0.9235 Hunter-Gaston Discriminatory Index (HGDI) with the 15-locus variable-number tandem repeats of mycobacterial interspersed repetitive units typing was improved to a 0.9574 HGDI with proposed 24-locus MLVA, thereby resulting in the subdivision of a large cluster of Beijing strains (SIT1) into 17 subclusters. We identified the spread of drug-resistant TB clones caused by three different MLVA types in the Beijing strain (SIT1) and a specific clone of XDR-TB caused by a rare genotype, the Manu-ancestor strain (SIT523). Overall, 49.5% of all isolates were clustered. These findings suggest that a remarkable transmission of drug-resistant TB occurred in Thailand. The remaining 50% of drug-resistant TB isolates were unique genotypes, which may have arisen from the individual acquisition of drug resistance. Our results suggest that transmitted and acquired resistance have played an equal role in the emergence of drug-resistant TB. Further characterization of whole genome sequences of clonal strains could help to elucidate the mycobacterial genetic factors relevant for drug resistance, transmissibility and virulence.

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“…The single nucleotide polymorphism (SNP)-based genotyping of 1,282 PTB and 132 EPTB patients in Thailand showed that sequence types (STs) 10 and 22 were found more frequently in EPTB patients (31.8% for ST10 and 10.6% for ST22) than in PTB patients (14.1% for ST10 and 5.5% for ST22) [15]. Nevertheless, ST19 and ST24 were found more frequently in PTB patients (13.9% for ST19 and 8.1% for ST24) than in EPTB patients (6.8% for ST19 and 4.5% for ST24) [17]. Spoligotyping of 113 bone and joint TB cases reported in China showed that 87.6% (99/113) isolates were identified as Beijing genotype [18].…”

Section: Discussionmentioning

confidence: 99%

Pulmonary and testicular tuberculosis in one patient caused by Mycobacterium tuberculosis with different genotypes

Lan

Chen

Zhang

2019

J Infect Dev Ctries

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Extrapulmonary tuberculosis (EPTB) accounted for 14% of 6.4 million cases of TB that were reported to WHO in 2017, and genitourinary TB (GUTB) is the second most common type of EPTB. The most common site of GUTB is the kidneys and testicular TB is relatively rare. The case of one patient with pulmonary and testicular TB caused separately by two different genotypes of Mycobacterium tuberculosis (Mtb) is further rare. Here, we present an unusual case of TB in which pulmonary TB (PTB) and testicular TB were caused by Mtb isolates with two different genotypes in a 91-year-old male patient from Zunyi, Guizhou Province of China. A better understanding of the mechanism by which a small number of tubercle bacilli are spread from the primary site of PTB to more distant parts/organs of the body, and what factors determine the potential EPTB site will provide us with new ways to prevent and control EPTB infections.

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Genetic Diversity and Dynamic Distribution of Mycobacterium tuberculosis Isolates Causing Pulmonary and Extrapulmonary Tuberculosis in Thailand

Cited by 22 publications

References 38 publications

Spoligotyping of Mycobacterium tuberculosis isolates at a tertiary care hospital in India

Spoligotyping of Mycobacterium tuberculosis isolates at a tertiary care hospital in India

Genotypic diversity of multidrug-, quinolone- and extensively drug-resistant Mycobacterium tuberculosis isolates in Thailand

Pulmonary and testicular tuberculosis in one patient caused by Mycobacterium tuberculosis with different genotypes

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