Genetic diversity and drug susceptibility profile of Mycobacterium tuberculosis isolated from different regions of India

Singh, Jitendra; Sankar, Manimuthu Mani; Kumar, Parveen; Couvin, David; Rastogi, Nalin; Singh, Sarman; Katoch, Vishwa Mohan; Chauhan, Deepika; Katoch, Kiran; Rodrigues, Camila; Lakshmi, V; Taori, Girdhar M.; Daginawala, Hatim F.; Singh, Rajpal; Bhattacharya, Basudev; Choudhury, Bandana; Singh, Namit; Devi, Uma K.; Swaminathan, Soumya

doi:10.1016/j.jinf.2015.04.028

Cited by 37 publications

(45 citation statements)

References 37 publications

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“…Lineages 2 (East Asian or Beijing) and 4 (Euro-American) are most common in Europe, Africa, and many other parts of the world [4–6]. Within India, lineage 3 predominates in the North and Northwest, whereas lineage 1 is common in the South but is found at low frequency in other parts of the country [3, 7–9]. In contrast, lineage 2 has been reported at similar prevalence throughout India (17%), though it does predominate in some Northeast states [7].…”

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confidence: 99%

“…Previous work has shown that Indian M. tuberculosis strains contain unique drug resistance mutations [20, 21], and that drug resistance patterns can vary among different lineages in India. For example, lineage 2 strains are more frequently MDR than lineage 1 or 3 strains [7, 22–25], and lineage 3 strains have been reported as more resistant than lineage 1 strains [7, 8, 26]. …”

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Mycobacterium tuberculosis Whole Genome Sequences From Southern India Suggest Novel Resistance Mechanisms and the Need for Region-Specific Diagnostics

Manson

Abeel

Galagan

et al. 2017

Clinical Infectious Diseases

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Mycobacterium tuberculosis Whole Genome Sequences From Southern India Suggest Novel Resistance Mechanisms and the Need for Region-Specific Diagnostics

Manson

Abeel

Galagan

et al. 2017

Clinical Infectious Diseases

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“…In addition, there is increasing evidence that certain genotypes of M. tuberculosis are associated with drug resistance, virulence, and disease progression [6]. Hence, the knowledge regarding strain diversity within the patients has special contemplation during TB epidemics or suspected outbreaks, thereby providing new insights for establishing strategic measures for TB control and prevention [7, 8]. …”

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confidence: 99%

First Insight into the Molecular Epidemiology ofMycobacterium tuberculosisIsolates from the Minority Enclaves of Southwestern China

Chen

Yang

et al. 2017

BioMed Research International

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Yunnan is a province located in southwestern China. The aim of this study was to investigate the genetic diversity of Mycobacterium tuberculosis (MTB) strains circulating in Yunnan Province. We used spoligotyping and a 12-locus high-resolution VNTR set to identify a total of 271 MTB isolates collected from six prefectures' Yunnan. All the 271 patients were classified as 11 different ethnic groups, including 133 Han patients (49.1%) and 138 minority patients (50.9%). Spoligotyping analyses revealed that the largest two spoligotypes were SIT1 (Beijing family, n = 136) and SIT53 (T family, n = 35). Statistical analysis indicated that the proportion of Beijing genotype in Qujing was significantly higher than that in the ethnic enclaves (P ≤ 0.01). Compared with the percentage of Beijing-family isolates from patients under 25 years of age (72.7%), those from patients aged 45~64 years (44.6%, P < 0.01) and older than 64 years (48.1%) (P = 0.04) were significantly lower. Beijing genotype strains (23.8%, 36/151) showed higher clustering rate than non-Beijing genotype strains (16/120, 13.3%, P = 0.029). In conclusion, our data demonstrated that the Beijing genotype was the predominant genotype in Yunnan Province. The distribution of Beijing genotype strains showed geographic diversity. In addition, Beijing genotype was associated with recent transmission rather than drug resistance.

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“…In the current study, geospatial analysis demonstrated variable distribution of strain clusters in the different districts of the study area suggesting areas affected with possible recent transmission. Spoligotyping may correctly identify Mtb complex in to various lineages and sub-lineages, however it is known to overestimate clustering of isolates due to its inferior discriminatory power compared to other genotyping techniques (10,30,31). In general, clustering of strains is a marker for recent transmission and can also serve to evaluate the performance of the TB control program (32) and help determine where to target interventions.…”

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confidence: 99%

Molecular Epidemiology of Pulmonary Tuberculosis in Southern Ethiopia

Merid

Hailu

Habtamu

et al. 2020

Preprint

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BackgroundUnderstanding the epidemiology of tuberculosis (TB) is limited by lack of genotyping data. We sought to characterize the drug susceptibility testing (DST) patterns and genetic diversity of M. tuberculosis (Mtb) isolates in Southern Ethiopia. MethodologyA cross-sectional study was conducted among newly diagnosed sputum smear positive patients with TB visiting nine health facilities in southern Ethiopia from June 2015 to May 2016. Three consecutive sputum samples (spot-morning-spot) per patient were examined using acid-fast bacilli (AFB) smear microscopy with all smear positive specimens having AFB cultures performed. Mtb isolates had DST performed using indirect proportion method and were genotyped with RD9 deletion typing and spoligotyping. Spoligotyping International Types (SIT) and sub-lineages (clades) were assigned according to the SITVITWEB data base. ResultsAmong 250 newly diagnosed patients with TB, 154 (52%) were male and 143 (57%) from rural areas.The prevalence of HIV co-infection was 4%. Of the 250 AFB positive sputum samples, 230 (92%) were culture positive. All 230 isolates were M. tuberculosis strains belonging to three lineages: Euro-American, 187 (81%); East-African-Indian, 31 (14%); and Lineage 7 (Ethiopian lineage), 8 (4%). The 230 isolates could be categorized into 65 different spoligotype patterns, of which 84% fell into 29 clusters. The dominant spoligotypes were SIT149 (21%), SIT53 (19%) and new strains (16%). Mtb strains were clustered by districts. DST revealed that 14% of Mtb isolates were resistant to > 1 first line anti-TB drugs including 11% to isoniazid. SIT 149 was the most prevalent genotype among drug resistant isolates (20%). ConclusionThe study revealed several clusters including lineage 7 strains circulating in southern Ethiopia. SIT 149 (T3-ETH) was the most dominant circulating strain in the study area including among drugresistant cases.

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Genetic diversity and drug susceptibility profile of Mycobacterium tuberculosis isolated from different regions of India

Cited by 37 publications

References 37 publications

Mycobacterium tuberculosis Whole Genome Sequences From Southern India Suggest Novel Resistance Mechanisms and the Need for Region-Specific Diagnostics

Mycobacterium tuberculosis Whole Genome Sequences From Southern India Suggest Novel Resistance Mechanisms and the Need for Region-Specific Diagnostics

First Insight into the Molecular Epidemiology ofMycobacterium tuberculosisIsolates from the Minority Enclaves of Southwestern China

Molecular Epidemiology of Pulmonary Tuberculosis in Southern Ethiopia

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