Genetic diversity and cryptolepine concentration of Cryptolepis sanguinolenta (Lindl). Schlt. from selected regions of Ghana

Amissah, Jacqueline Naalamle; Spiller, Monika; Oppong, Allen; Osei‐Safo, Dorcas; Owusu-Darko, Rodney; Debener, Thomas; Danquah, Eric Yirenkyi; Addae‐Mensah, Ivan

doi:10.1016/j.jarmap.2015.12.005

Cited by 2 publications

(4 citation statements)

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“…The study finding agrees with the report by Amissah et al (2016) that used AFLP analysis to reveal a low (25%) genetic diversity in 116 sampled plants. Additional samples and analyses need to be completed to confirm our preliminary findings using microsatellite analysis.…”

Section: Resultssupporting

confidence: 92%

“…The harvested roots are sold in local markets.Nonsustainable destructive harvesting of the plants for the roots has resulted in a substantial decrease in wild populations (Jansen and Schmelzer, 2010). Its widespread use as a medicinal plant coupled with over-harvesting, calls for the formulation of effective management plans and conservation through cultivation with the ultimate goal of selecting high active ingredient producing plants for breeding (Amissah et al, 2016). There is very limited information about the genetic diversity in populations of C. sanguinolenta.…”

Section: Introductionmentioning

confidence: 99%

“…There is very limited information about the genetic diversity in populations of C. sanguinolenta. Amplified Fragment Length Polymorphism (AFLP) revealed low genetic diversity among 116 plants sampled from three regions in Ghana, but did not characterize the population structure or gene flow within the collected sites (Amissah et al, 2016). Microsatellites or single sequence repeats (SSRs) are codominant molecular markers, as compared to dominant AFLPs markers, that typically are polymorphic (Gupta et al, 1996) and well-suited for studying population genetic diversity and dynamics.…”

Section: Introductionmentioning

confidence: 99%

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Characterization of thirteen microsatellite loci from the Ghanian antimalarial plant Cryptolepis sanguinolenta

Amissah¹,

Wadl²,

Hadziabdic³

et al. 2016

J. Med. Plants Res.

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Cryptolepis sanguinolenta (Lindl.) Schlechter (Periplocaceae) is an herbaceous plant used in traditional medicine to treat malaria and populations of the species are diminishing due to overharvesting and lack of conservation. Codominant microsatellite markers that can be used to characterize genetic diversity and population structure are currently not available. Therefore, the study isolated 75 microsatellite loci from genomic sequence data, which were then screened for the ability to reveal polymorphisms. From the 75 candidate loci, 13 polymorphic microsatellite loci were optimized for future population genetics studies. Twenty-two C. sanguinolenta samples were collected from eight different geographical locations in Ghana. Alleles per locus ranged from 3 to 7 with a mean of 4.4. Expected heterozygosity ranged from 0.24 to 0.77, and all but one locus deviated significantly from Hardy-Weinberg equilibrium. Genetic differentiation mean was 0.06 among all loci, indicating relatively low genetic diversity in these samples. These microsatellite loci should be useful to study genetic diversity, gene flow and population structure as well as in a project involving breeding and conservation of C. sanguinolenta.

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Section: Resultssupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Characterization of thirteen microsatellite loci from the Ghanian antimalarial plant Cryptolepis sanguinolenta

Amissah¹,

Wadl²,

Hadziabdic³

et al. 2016

J. Med. Plants Res.

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“…Plants used in the study were identified based on morphological characteristics of the species. Accession and voucher specimen details are as indicated in [7]. Parent plants obtained from the root cuttings were sprayed with 0.3% systemic fungicide solution (Agriette 80 WP a.i.…”

Section: Explant Preparationmentioning

confidence: 99%

Influence of BA and IBA or NAA Combinations on Micropropagation of <i>Cryptolepis sanguinolenta</i>

Monney¹,

Amissah²,

Blay³

2016

AJPS

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The study being the first of its kind established an efficient protocol for micropropagation of Cryptolepis sanguinolenta, an important endangered medicinal plant species, used in the treatment of Malaria. For shoot induction, semi hard wood nodal segments were maintained on MS (Murashige and Skoog) nutrient medium supplemented with MS vitamins, 30 g/L sucrose, 3% gelrite and various auxin and cytokinin combinations. Treatments involved 6-benzyladenine (BA) at 0.5, 1.0, 1.5, 2.0, 2.5 or 3.0 mg/L in combination with 0.1 mg/L Indole 3-butyric acid (IBA) or Naphthaleneacetic acid (NAA). Control consisted of hormone free MS medium. BA and IBA combinations were found to be more efficient in shoot regeneration than the BA and NAA combinations. Cultures maintained on MS medium supplemented with 3 mg/L BA, in combination with 0.1 mg/L IBA recorded the highest shoot induction (100%), mean shoot length (1.28 cm) and mean number of nodes per explant (2.5). This, however, did not differ significantly from cultures maintained on 2 or 2.5 mg/L BA and 0.1 mg/L IBA supplemented MS medium. Regenerated shoots were transferred onto different media for root induction. Treatments consisted of full strength MS medium augmented with either 0.5 or 0.1 mg/L IBA, hormone free half strength MS medium and half strength MS medium augmented with 0.01 mg/L IBA. After six weeks of culture, no rooting was recorded in all treatments with the exception of half strength MS medium supplemented with 0.01 mg/L IBA, which recorded 60% rooting. Regenerated plantlets were successfully weaned and established in the greenhouse. Keywords C. sanguinolenta, Micropropagation, Plant Growth Regulators M. A. D. Monney et al. 573

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Genetic diversity and cryptolepine concentration of Cryptolepis sanguinolenta (Lindl). Schlt. from selected regions of Ghana

Cited by 2 publications

References 9 publications

Characterization of thirteen microsatellite loci from the Ghanian antimalarial plant Cryptolepis sanguinolenta

Characterization of thirteen microsatellite loci from the Ghanian antimalarial plant Cryptolepis sanguinolenta

Influence of BA and IBA or NAA Combinations on Micropropagation of <i>Cryptolepis sanguinolenta</i>

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Genetic diversity and cryptolepine concentration of Cryptolepis sanguinolenta (Lindl). Schlt. from selected regions of Ghana

Cited by 2 publications

References 9 publications

Characterization of thirteen microsatellite loci from the Ghanian antimalarial plant Cryptolepis sanguinolenta

Characterization of thirteen microsatellite loci from the Ghanian antimalarial plant Cryptolepis sanguinolenta

Influence of BA and IBA or NAA Combinations on Micropropagation of &lt;i&gt;Cryptolepis sanguinolenta&lt;/i&gt;

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Influence of BA and IBA or NAA Combinations on Micropropagation of <i>Cryptolepis sanguinolenta</i>