Genetic Diversity and Core Collection Evaluations in Common Wheat Germplasm from the Northwestern Spring Wheat Region in China

Hao, Chenyang; Zhang, X. Y.; Wang, L. F.; Dong, Yushen; Shang, Xianwen; Jia, Jizeng

doi:10.1007/s11032-005-2453-6

Cited by 85 publications

(72 citation statements)

References 12 publications

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“…PIC value which is considered as one of the most reliable parameters to detect diversity assessed by any marker ranged from 8% to 63.2% for 49 SSR primers. Hao et al (2006) also suggested that to evaluate the genetic diversity in any germplasm the PIC value and number of alleles per locus must be considered. Huang (2002) and Hai et al (2007) proved that there is positive correlation between the number of alleles per locus and PIC value.…”

Section: Discussionmentioning

confidence: 99%

Assessment of D-genome Based Genetic Diversity in Drought Tolerant Wheat Germplasm

Faheem¹,

Mahmood²,

Shabbir³

et al. 2015

IJAB

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The complex nature of drought stress is one of the key bottlenecks in wheat improvement. Besides conventional breeding approaches for wheat improvement under water deficit conditions use of wild relatives like Aegilops tauschii (DD), as a source of rich genetic variation has significantly triggered the process. In this study, we have assessed the D-genome based genetic diversity in drought tolerant germplasm by using SSR markers. A total number of 178 alleles were detected on 49 loci with an average of 3.49 alleles per locus with the highest number being of 10 alleles for marker gwm515-2D. Genetic diversity explained by these SSR makers ranged from 4 to 67% with an average of 56.2% per marker. There were 23 SSR markers, which revealed PIC (Polymorphism Information Content) values of more than 50% while overall the PIC values for all markers ranged from 8 to 63.2% with an average of 48.1%. Clustering of genotypes D41, D43, D44, D50 and D51 within two well-known drought tolerant cultivars viz. Dharwar Dry and Sitta showed the suitability of these genotypes for water deficit areas. In conclusion, the genetic diversity present in the studied germplasm may be used in wheat breeding programs targeted for the drought tolerance especially in arid/semi-arid (rain-fed) environments.

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Section: Discussionmentioning

confidence: 99%

Assessment of D-genome Based Genetic Diversity in Drought Tolerant Wheat Germplasm

Faheem¹,

Mahmood²,

Shabbir³

et al. 2015

IJAB

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“…Salah satu penanda molekuler berbasis PCR yang sangat popular saat ini adalah Simple sequence repeat (SSR). Penanda ini telah digunakan untuk mempelajari keragaman genetik pada berbagai tanaman, seperti gandum (Liu et al, 2007;Hao et al, 2006), barley (Brantestam et al, 2007), cole (Hasan et al, 2006), jowar (Marco et al, 2007), winter triticale (Tams et al, 2004), dan kopi (Aggarwal et al, 2007). Selain relatif sederhana dan murah, teknik ini juga lebih reproduksibel, bersifat kodominan dan multi alelik, melingkupi daerah genom yang luas, serta pada umumnya bersifat polimorfik (Saha et al, 2005;Gouvêa et al, 2010).…”

Section: Pendahuluanunclassified

Evaluasi 18 primer SSR untuk pengembangan sidikjari DNA tanaman karet (Hevea brasiliensis Muell. Arg.) Evaluation of 18 SSR primers to develop DNA fingerprint of rubber tree (Hevea brasiliensis Muell. Arg.)

BUDIANI¹,

WOELAN²,

MINARSIH³

et al. 2016

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PR 107, PR 261, SP 217, PB 330, PB 340, IRR 5, IRR 112, IRR 118, IRR 220, GT 1, BPM 101 and RRIM 712. Each clone can be distinguished from each other using only one primer pair. Identification of the other tree clones (PB 5/51, PB 260, and RRIC 110) AbstrakPemuliaan tanaman karet untuk menghasilkan klon-klon unggul baru menghadapi masalah lamanya siklus seleksi. Di sisi lain, upaya peningkatan produksi melalui pembukaan lahan baru, juga terkendala oleh ketersediaan bibit, yang memicu beredarnya bibit palsu, yang umumnya tidak jelas asal usulnya. Oleh karena itu, diperlukan ketersediaan marka yang dapat digunakan sebagai sidikjari DNA bagi klon-klon tanaman karet yang ada, sehingga dapat membantu mempercepat proses seleksi dan mengetahui kemurnian bibit. Penelitian ini bertujuan untuk mengevaluasi 18 primer SSR yang telah dipublikasikan untuk mengidentifikasi 17 klon karet. DNA yang murni diisolasi dari 17 klon, kemudian dilakukan optimasi suhu annealing untuk setiap jenis primer agar diperoleh hasil amplifikasi terbaik. Pada awal percobaan hasil PCR dicek pada gel agarosa dan gel poliakrilamida, namun analisis untuk seluruh hasil PCR dilakukan pada gel SDS poliakrilamid, karena gel ini secara nyata dapat memisahkan fragmen DNA yang hanya berbeda beberapa basa. Hasil percobaan menunjukkan bahwa 14 klon dapat diidentifikasi secara spesifik menggunakan 11 primer. Empat dari 18 pasang primer yang diuji dapat mengidentifikasi 12 klon yang dianalisis, yaitu PR 107, PR 261, SP 217, PB 330, PB 340,. IRR 5, IRR 112, IRR 118, IRR 220, GT 1, BPM 101 dan RRIM 712. Masing-maing klon tersebut dapat dibedakan dari klon lainnya hanya dengan menggunakan satu jenis primer. Sedangkan identifikasi tiga klon lainnya (PB 5/51, PB 260, dan RRIC110) harus dilakukan dengan menggabungkan hasil PCR menggunakan beberapa primer. Meskipun hasil percobaan ini menunjukkan bahwa marka SSR sangat berpotensi untuk digunakan sebagai sidikjari DNA klon-klon karet, namun primer yang sama perlu diuji untuk klon-klon lainnya. Demikian pula primer lain perlu diuji untuk mengidentifikasi klon-klon yang belum teridentifikasi menggunakan 18 primer dalam penelitian ini.

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“…SSR molecular markers have been widely used to distinguish crop genotypes (Sun et al, 1999;Virk et al, 1999;Eujayl et al, 2001;Eujayl et al, 2002) and study genetic diversity (Song et al, 2003;Hao et al, 2006;Liu et al, 2007;Caniato et al, 2007). In recent years, a large number of EST-and gSSR molecular markers for many crops were developed (Davierwala et al, 2001;Varshney et al, 2005;Aggarwal et al, 2007).…”

Section: The Polymorphism Of Est-and Gssrmentioning

confidence: 99%

“…Simple sequence repeat (SSR) marker has been used as an ideal molecular marker to investigate the genetic diversity because of its multi-allelic nature, reproducibility, codominant inheritance, high abundance and extensive genome coverage (Gupta & Varshney, 2000) in many crops, such as rubber tree (Lekawipat et al, 2003;Saha et al, 2005;Gouvêa et al, 2010), wheat (Liu et al, 2007;Hao et al, 2006), bean (Choi et al, 1999), barley (Brantestam et al, 2007), cole (Hasan et al, 2006), jowar (Marco et al, 2007), triticale (Tams et al, 2004), rice (Song et al, 2003) and coffee (Aggarwal et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Analysis of Genetic Diversity and SSR Allelic Variation in Rubber Tree (Hevea brasilensis)

Feng¹,

Wu²,

Li³

et al. 2012

The Molecular Basis of Plant Genetic Diversity

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Genetic Diversity and Core Collection Evaluations in Common Wheat Germplasm from the Northwestern Spring Wheat Region in China

Cited by 85 publications

References 12 publications

Assessment of D-genome Based Genetic Diversity in Drought Tolerant Wheat Germplasm

Assessment of D-genome Based Genetic Diversity in Drought Tolerant Wheat Germplasm

Evaluasi 18 primer SSR untuk pengembangan sidikjari DNA tanaman karet (Hevea brasiliensis Muell. Arg.) Evaluation of 18 SSR primers to develop DNA fingerprint of rubber tree (Hevea brasiliensis Muell. Arg.)

Analysis of Genetic Diversity and SSR Allelic Variation in Rubber Tree (Hevea brasilensis)

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