Genetic diversity and characterization of M. tuberculosis isolates causing extrapulmonary tuberculosis in Bangladesh

Uddin, Mohammad Khaja Mafij; Ather, Md. Fahim; Rahman, Ananna; Nasrin, Rumana; Rahman, S. M. Mazidur; Kabir, Shahjahan; Chedid, Carole; Ahmed, Shahriar

doi:10.1016/j.meegid.2021.105052

Cited by 6 publications

(5 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This finding was in agreement with the studies carried out in Gondar and Dessie [ 14 , 15 ], and could be due to underreporting, because of social and cultural factors. The finding differed from the previous study conducted in Bangladesh [18] , which could be due to a relatively lower sample size and various social factors. The presence of contact history with known TB patients increased the chance of becoming diseased to four times more than for those who had no contact history.…”

Section: Discussioncontrasting

confidence: 99%

“…Our study showed a higher culture prevalence of tuberculosis among presumptive EPTB patients than the study conducted in Dessie (8.8%) [17] , which could be due to sociodemographic differences among the study participants. The culture prevalence was also higher than in a study conducted in Bangladesh (10.5%) [18] . One possible reason for this difference was the solid culture technique used in the latter study, which has a relatively lower sensitivity compared with liquid culture.…”

Section: Discussioncontrasting

confidence: 73%

See 1 more Smart Citation

Burden of Mycobacterium tuberculosis and Associated Factors among Presumptive Extra pulmonary Tuberculosis Patients from Selected Health Facilities, Addis Ababa, Ethiopia

Alehegn¹,

Gebreyohannes²,

Berhane³

et al. 2023

IJID Regions

View full text Add to dashboard Cite

Section: Discussioncontrasting

confidence: 99%

Section: Discussioncontrasting

confidence: 73%

Burden of Mycobacterium tuberculosis and Associated Factors among Presumptive Extra pulmonary Tuberculosis Patients from Selected Health Facilities, Addis Ababa, Ethiopia

Alehegn¹,

Gebreyohannes²,

Berhane³

et al. 2023

IJID Regions

View full text Add to dashboard Cite

“…Furthermore, five of seven patients who experienced extrapulmonary TB had been infected with LAM Mtb strains. Mtb isolates belonging to the LAM family had been acquired from extrapulmonary TB patients before, however, this Mtb genotype was not predominant in studied populations ( 64 , 65 ). On the contrary, each studied TB cluster of the T genotype involved only three patients, while isolates of three clusters belonged to spoligotypes that are not as widespread in Latvia (i.e., SIT3340 (two clusters) and SIT280).…”

Section: Discussionmentioning

confidence: 82%

Unraveling tuberculosis patient cluster transmission chains: integrating WGS-based network with clinical and epidemiological insights

Sadovska,

Ozere,

Pole

et al. 2024

Front. Public Health

View full text Add to dashboard Cite

BackgroundTuberculosis remains a global health threat, and the World Health Organization reports a limited reduction in disease incidence rates, including both new and relapse cases. Therefore, studies targeting tuberculosis transmission chains and recurrent episodes are crucial for developing the most effective control measures. Herein, multiple tuberculosis clusters were retrospectively investigated by integrating patients’ epidemiological and clinical information with median-joining networks recreated based on whole genome sequencing (WGS) data of Mycobacterium tuberculosis isolates.MethodsEpidemiologically linked tuberculosis patient clusters were identified during the source case investigation for pediatric tuberculosis patients. Only M. tuberculosis isolate DNA samples with previously determined spoligotypes identical within clusters were subjected to WGS and further median-joining network recreation. Relevant clinical and epidemiological data were obtained from patient medical records.ResultsWe investigated 18 clusters comprising 100 active tuberculosis patients 29 of whom were children at the time of diagnosis; nine patients experienced recurrent episodes. M. tuberculosis isolates of studied clusters belonged to Lineages 2 (sub-lineage 2.2.1) and 4 (sub-lineages 4.3.3, 4.1.2.1, 4.8, and 4.2.1), while sub-lineage 4.3.3 (LAM) was the most abundant. Isolates of six clusters were drug-resistant. Within clusters, the maximum genetic distance between closely related isolates was only 5–11 single nucleotide variants (SNVs). Recreated median-joining networks, integrated with patients’ diagnoses, specimen collection dates, sputum smear microscopy, and epidemiological investigation results indicated transmission directions within clusters and long periods of latent infection. It also facilitated the identification of potential infection sources for pediatric patients and recurrent active tuberculosis episodes refuting the reactivation possibility despite the small genetic distance of ≤5 SNVs between isolates. However, unidentified active tuberculosis cases within the cluster, the variable mycobacterial mutation rate in dormant and active states, and low M. tuberculosis genetic variability inferred precise transmission chain delineation. In some cases, heterozygous SNVs with an allelic frequency of 10–73% proved valuable in identifying direct transmission events.ConclusionThe complex approach of integrating tuberculosis cluster WGS-data-based median-joining networks with relevant epidemiological and clinical data proved valuable in delineating epidemiologically linked patient transmission chains and deciphering causes of recurrent tuberculosis episodes within clusters.

show abstract

“…Many of the isolates were found to belong to M. tuberculosis lineages, including EAI (51.5%), CAS (16.1%), and Beijing (4.4%), which are common in the country of origin of expatriates in Oman, such as the Indian subcontinent, South East Asia, and East Africa ( 40 ). Whereas the other lineages, T, LAM, and H (27.9%) are prevalent in East Africa, which has a historical link with Oman ( 41 ).…”

Section: Discussionmentioning

confidence: 99%

Mycobacterium tuberculosis epidemiology in Oman: whole-genome sequencing uncovers transmission pathways

Babiker,

Al-Jardani,

Al-Azri

et al. 2023

Microbiol Spectr

View full text Add to dashboard Cite

Tuberculosis (TB) originating from expatriates that hail from high TB-burden countries is hypothesized to play a role in continued TB transmission in Oman. Here, we used whole-genome sequencing (WGS) to assess national TB transmission dynamics. The annual incidence per 100,000 population per year was calculated for nationals and expatriates. A convenience sample of Mycobacterium tuberculosis (MTB) isolates from 2018 to 2019 was sequenced and analyzed with publicly available TB sequences from Bangladesh, Tanzania, the Philippines, India, and Pakistan. Relatedness was assessed by generating core-genome single nucleotide polymorphism (SNP) distances. The incidence of TB was five cases per 100,000 persons in 2018 and seven cases per 100,000 persons in 2020 ( R 2 = 0.34, P = 0.60). Incidence among nationals was 3.9 per 100,000 persons in 2018 and 3.5 per 100,000 persons in 2020 ( R 2 = 0.20 , P = 0.70), and incidence among expatriates was 7.2 per 100,000 persons in 2018 and 12.7 per 100,000 persons in 2020 ( R 2 = 0.74, P = 0.34). Sixty-eight local MTB isolates were sequenced and analyzed with 393 global isolates. Isolates belonged to nine distinct spoligotypes. Two isolates, originating from an expatriate and an Omani national, were grouped into a WGS-based cluster (SNP distance < 12), which was corroborated by an epidemiological investigation. Relatedness of local and global isolates (SNP distance < 100) was also seen. The relatedness between MTB strains in Oman and those in expatriate countries of origin can aid inform TB control policy. Our results provide evidence that WGS can complement epidemiological analysis to achieve the End TB strategy goal in Oman. IMPORTANCE Tuberculosis (TB) incidence in Oman remains above national program control targets. TB transmission originating from expatriates from high TB-burden countries has been hypothesized to play a role. We used whole-genome sequencing (WGS) to assess TB transmission dynamics between expatriates and Omani nationals to inform TB control efforts. Available Mycobacterium tuberculosis isolates from 2018 to 2019 underwent WGS and analysis with publicly available TB sequences from Bangladesh, the Philippines, India, and Pakistan to assess for genetic relatedness. Our analysis revealed evidence of previously unrecognized transmission between an expatriate and an Omani national, which was corroborated by epidemiological investigation. Analysis of local and global isolates revealed evidence of distant relatedness between local and global isolates. Our results provide evidence that WGS can complement classic public health surveillance to inform targeted interventions to achieve the End TB strategy goal in Oman.

show abstract

Genetic diversity and characterization of M. tuberculosis isolates causing extrapulmonary tuberculosis in Bangladesh

Cited by 6 publications

References 35 publications

Burden of Mycobacterium tuberculosis and Associated Factors among Presumptive Extra pulmonary Tuberculosis Patients from Selected Health Facilities, Addis Ababa, Ethiopia

Burden of Mycobacterium tuberculosis and Associated Factors among Presumptive Extra pulmonary Tuberculosis Patients from Selected Health Facilities, Addis Ababa, Ethiopia

Unraveling tuberculosis patient cluster transmission chains: integrating WGS-based network with clinical and epidemiological insights

Mycobacterium tuberculosis epidemiology in Oman: whole-genome sequencing uncovers transmission pathways

Contact Info

Product

Resources

About