Genetic diversity and biogeography of red turpentine beetle Dendroctonus valens in its native and invasive regions

Abstract

Genetic diversity and biogeography of RTB 291 291Genetic diversity and biogeography of red turpentine beetle Dendroctonus valens in its native and invasive regions Abstract Sequences of 479 bp region of the mitochondrial COI gene were applied to detect population genetic diversity and structure of Dendroctonus valens populations. By comparing the genetic diversity between native and invasive populations, it was shown that the genetic diversity of Chinese populations was obviously lower than that of native populations with both indices of haplotype diversity and Nei's genetic diversity, suggesting genetic bottleneck occurred in the invasive process of D. valens, and was then followed by a relatively quick population buildup. According to phylogenetic analyses of haplotypes, we suggested that the origin of the Chinese population was from California, USA. Phylogenetic and network analysis of native populations of D. valens revealed strong genetic structure at two distinct spatial and temporal scales in North America. The main cause resulting in current biogeographic pattern was supposedly due to recycled glacial events. Meanwhile, a cryptic species might exist in the Mexican and Guatemalan populations.

Large Shift in Symbiont Assemblage in the Invasive Red Turpentine Beetle

“…Furthermore, the evidence strongly supports the notion that the source would not have been from WNA where large collections of the beetle have failed to yield the fungus, and rather from an area in ENA where L. procerum is commonly associated with the beetle. This study is in contrast to the view from previous studies based on COI [51,52] that D. valens was most likely introduced into China from WNA. Clearly, further population genetics studies including larger numbers of samples of D. valens from ENA will be needed to clarify the invasion history of D. valens .…”

“…In the 1980’s, D. valens invaded China (Figure 1), where it aggressively kills pine species native to China [50]. Population genetics studies on D. valens using cytochrome oxidase I (COI) led to the suggestion that D. valens was most likely introduced from the Pacific Northwest in WNA [51,52]. However, both studies included only one population of D. valens from ENA, in the U.S. state of Michigan.…”

Genetic analyses of the Asian longhorned beetle (Coleoptera, Cerambycidae, Anoplophora glabripennis), in North America, Europe and Asia

“…Although not without limitations (e.g., see Ballard and Whitlock 2004), mitochondrial DNA data has provided important insights into the history of invasive insect populations (Grapputo et al 2005;Scheffer and Grissell 2003;Puillandre et al 2008;Cai et al 2008). We analyzed DNA samples from invasive populations of A. glabripennis in North America using mitochondrial DNA sequence data to evaluate genetic diversity and relatedness within and among these introduced populations.…”

Rapid Detection of Red Turpentine Beetle (Dendroctonus valensLeconte) Using Nested PCR

“…Lane 1-6 represents 40 ng, 4 ng, 400 pg, 40 pg, 4 pg and 400 fg plasmid DNA of D. valens, respectively; lanes 7-10 represent 40 ng plasmid DNA of S. schevyrewi, X. artecomans, X. interjectus and B. minor respectively; lane 11 represents the negative control. 2005; Cai et al, 2008;Yao, 2010), it has become a reliable gene candidate for research in species identification. Primers used in the assay were designed based on the COI gene of D. valens.…”