Genetic diversity and association of molecular markers with biochemical traits in Tunisian olive cultivars

Omri, Amal; Abdelhamid, Sofiane; Benincasa, Cinzia; Araouki, Amira; Ayadi, Mohamed Ali; Gharsallaoui, Mariem; Gouiaa, Mohamed

doi:10.1007/s10722-020-01058-4

Cited by 8 publications

(4 citation statements)

References 33 publications

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“…Similar results to ours were reported in a recent study by Omri et al [ 45 ], who observed a lack of similarity between the clusters generated from genetic data, compared to physicochemical data. In specific, they calculated Nei’s gene diversity h from 36 RAPD and 94 ISSR polymorphic bands, and clustered 19 Tunisian cultivars.…”

Section: Resultssupporting

confidence: 92%

A Study on the Clustering of Extra Virgin Olive Oils Extracted from Cultivars Growing in Four Ionian Islands (Greece) by Multivariate Analysis of Their Phenolic Profile, Antioxidant Activity and Genetic Markers

Kalaboki

Koulougliotis

Kleisiari

et al. 2021

Foods

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Background: The phenolic fraction of extra virgin olive oil (EVOO) has disease preventive and health-promoting properties which are supported by numerous studies. As such, EVOO is defined as a functional food. The aim of the present study was to characterize the phenolic profile of olive oil from cultivars farmed in the Ionian Islands (Zakynthos, Kefalonia, Lefkada, and Kerkyra) and to investigate the association of phenols to antioxidant activity, which is central to its functionality. Furthermore, the study investigates whether multivariate analyses on the concentration of individual biophenolic compounds and genetic population diversity could classify the olive oil samples based on their geographic origin. Methods: Phenols were determined in 103 samples from different Ionian Island tree populations by 1H nuclear magnetic resonance (NMR), and sample antioxidant activity was measured by their capacity to reduce the free radical 2,2-diphenyl-1-picrylhydrazyl) (DPPH). Genetic diversity was measured by estimating Nei’s population genetic distance using 15 reproducible bands from random amplified polymorphic DNA (RAPD) genotyping. Results: Principal component analysis (PCA) of the secoiridoid concentrations clustered samples according to cultivar. Clustering based on genetic distances is not concordant with phenolic clustering. A cultivar effect was also demonstrated in the association between the concentration of individual phenols with DPPH reducing activity. Conclusions: Taken together, the study shows that the olive oil phenolic content defines “cultivar-specific phenolic profiles” and that environmental factors other than agronomic conditions contribute more to phenotype variance than genetics.

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Section: Resultssupporting

confidence: 92%

A Study on the Clustering of Extra Virgin Olive Oils Extracted from Cultivars Growing in Four Ionian Islands (Greece) by Multivariate Analysis of Their Phenolic Profile, Antioxidant Activity and Genetic Markers

Kalaboki

Koulougliotis

Kleisiari

et al. 2021

Foods

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“…However, our study is the first one to use SMRA to identify markers associated with antioxidant properties. This method can provide easy and reliable identification of favourable genotypes or populations in a breeding program at an early stage and has been used to associate molecular markers with traits in numerous species such as mulberry [ 83 ], buckthorn [ 84 ], and Tunisian olive [ 85 ]. This approach is a convenient and quick tool for marker–trait association, without the need to map populations.…”

Section: Discussionmentioning

confidence: 99%

Genetic Diversity of Blueberry Genotypes Estimated by Antioxidant Properties and Molecular Markers

Bhatt

Debnath

2021

Antioxidants

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Blueberries (Vaccinium spp.) have gained much attention worldwide because of their potential health benefits and economic importance. Genetic diversity was estimated in blueberry hybrids, wild clones and cultivars by their antioxidant efficacy, total phenolic and flavonoid contents, and express sequence tag–simple sequence repeat (SSR) (EST–SSR), genomic (G)–SSR and express sequence tag–polymerase chain reaction (EST–PCR) markers. Wide diversity existed among the genotypes for antioxidant properties, with the highest variation for DPPH radical scavenging activity (20-fold), followed by the contents of total flavonoids (16-fold) and phenolics (3.8-fold). Although a group of 11 hybrids generated the maximum diversity for antioxidant activity (15-fold), wild clones collected from Quebec, Canada, had the maximum variation for total phenolic (2.8-fold) and flavonoid contents (6.9-fold). Extensive genetic diversity was evident from Shannon’s index (0.34 for EST–SSRs, 0.29 for G–SSR, 0.26 for EST–PCR) and expected heterozygosity (0.23 for EST–SSR, 0.19 for G–SSR, 0.16 for EST–PCR). STRUCTURE analysis separated the genotypes into three groups, which were in agreement with principal coordinate and neighbour-joining analyses. Molecular variance suggested 19% variation among groups and 81% among genotypes within the groups. Clustering based on biochemical data and molecular analysis did not coincide, indicating a random distribution of loci in the blueberry genome, conferring antioxidant properties. However, the stepwise multiple regression analysis (SMRA) revealed that 17 EST–SSR, G–SSR and EST–PCR markers were associated with antioxidant properties. The study is valuable to breeding and germplasm conservation programs.

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“…The molecular markers employed most often are SSRs (simple sequence repeats) and SNPs (single nucleotide polymorphisms) [72]. In many studies, SSR and SNP markers have been used to discriminate different olive varieties in reference to their origin as well as morphological and biochemical traits [72][73][74][75][76]. Recent studies include the development of the most complete genomic variation map and the most thorough resource of molecular variation until today, through SNP and genome-wide association studies (GWAS), for 89 olive tree genotypes derived from the Mediterranean Basin [77].…”

Section: Genomicsmentioning

confidence: 99%

Adaptation Mechanisms of Olive Tree under Drought Stress: The Potential of Modern Omics Approaches

Nteve,

Kostas,

Polidoros

et al. 2024

Agriculture

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Olive (Olea europaea L.) is a crop of enormous economic and cultural importance. Over the years, the worldwide production of olive oil has been decreasing due to various biotic and abiotic factors. The current drop in olive oil production resulting from climate change raises concerns regarding the fulfillment of our daily demand for olive oil and has led to a significant increase in market prices. In the future, there will be a higher chance that we will face a severe shortage of olive oil, which could harm both the economic sector and the food supply. As olive groves cover more than 5 million hectares in the European Union alone, the need to preserve the crop in the context of extreme climatic events is imperative. As drought is considered one of the most limiting factors in agriculture, drought-resistant varieties and sustainable irrigation strategies are being developed to mitigate the impact of drought on crop productivity and secure the future supply of olive oil. This review focuses on recently gained insights into drought stress in olive trees through omics and phenomics approaches to unravelling mechanisms that may lead to developing new varieties that are tolerant against drought elicited by changes in growing systems.

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Genetic diversity and association of molecular markers with biochemical traits in Tunisian olive cultivars

Cited by 8 publications

References 33 publications

A Study on the Clustering of Extra Virgin Olive Oils Extracted from Cultivars Growing in Four Ionian Islands (Greece) by Multivariate Analysis of Their Phenolic Profile, Antioxidant Activity and Genetic Markers

A Study on the Clustering of Extra Virgin Olive Oils Extracted from Cultivars Growing in Four Ionian Islands (Greece) by Multivariate Analysis of Their Phenolic Profile, Antioxidant Activity and Genetic Markers

Genetic Diversity of Blueberry Genotypes Estimated by Antioxidant Properties and Molecular Markers

Adaptation Mechanisms of Olive Tree under Drought Stress: The Potential of Modern Omics Approaches

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